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Nerve growth factor loaded silk fibroin nanofiber scaffold and preparation method

A nerve growth factor and nanofiber technology, applied in the field of composite materials, can solve the problems of limiting the application of electrospinning tubular nanofiber materials, and achieve the effect of good molding effect, high efficiency and low cost

Inactive Publication Date: 2015-06-03
YANCHENG INST OF IND TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditionally, electrospinning usually uses a roller device to collect three-dimensional tubular nanofiber materials. The prepared three-dimensional pipes have great limitations in terms of macroscopic structure and microscopic shape control of fiber orientation, which greatly limits electrospinning. Applications of Tubular Nanofibrous Materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Put 80g of silk into 1000ml of 0.05% sodium carbonate aqueous solution, raise the temperature to 100°C, boil for 30min, repeat the boiling for 3 times to remove the sericin on the surface of the silk, take out the silk fibroin, rinse it with distilled water, and wash it Spread it on a stainless steel plate to form a thin layer, and place it at room temperature for more than 12 hours to obtain dry silk fibroin.

[0020] 2. Add the dried silk fibroin in batches to a lithium bromide (LiBr) solution with a concentration of 45-60% at a bath ratio of 1:10, place it in a water bath at 76±2°C and stir for more than 6 hours until it is completely dissolved.

[0021] 3. Pour the silk fibroin solution prepared in step 2 into a dialysis bag with an NWCO of 3.5KDA, dialyze with deionized water for 72 hours, change the water 7-8 times in the first 24 hours, and change the water 7-8 times in the middle 24 hours. In the last 24 hours, the water was changed 5 to 6 times to remove sma...

Embodiment 2

[0028] 1. Put 100g of silk raw silk into 1250ml of 0.05% sodium carbonate aqueous solution, raise the temperature to 100°C, boil for 0.5h, repeat the cooking 3 times, remove the sericin on the surface of the raw silk, remove the silk fibroin and use deionized water Rinse well, spread it on a plastic plate to form a thin layer, and place it at room temperature for more than 12 hours to obtain dry silk fibroin.

[0029] 2. Prepare the molar ratio as calcium chloride (CaCl 2 ): water (H 2 O): ethanol (C 2 h 5 OH) = 1:8:2 ternary mixed solvent, add the dry silk fibroin prepared in step 1 to the ternary mixed solvent in batches with a liquor ratio of 1:10, and place it in a water bath at 76±2°C for constant temperature stirring , until completely dissolved to obtain silk fibroin-calcium chloride solution.

[0030] 3. Pour the silk fibroin solution prepared in step 2 into a dialysis bag with an NWCO of 3.5KDA, dialyze with deionized water for 72 hours, change the water 7-8 times...

Embodiment 3

[0037] 1. Put 150g of silk scraps into 1875ml of 0.05% sodium carbonate (Na 2 CO 3 ) aqueous solution, heat up to 100°C, boil for 30 minutes, repeat the treatment 3 times to get rid of sericin on the surface of silk to obtain silk fibroin, remove the silk fibroin and rinse it with distilled water, spread it on a glass plate to form a thin layer , placed at room temperature for more than 12 hours to obtain dry silk fibroin.

[0038] 2. Prepare the molar ratio as calcium chloride (CaCl 2 ): water (H 2 O): ethanol (C 2 h 5 OH) = 1:8:2 ternary mixed solvent, add the dry silk fibroin prepared in step 1 to the ternary mixed solvent in batches with a bath ratio of 1:10, and then heat and stir in a water bath at 76±2°C Dissolved into silk fibroin solution.

[0039] 3. Pour the silk fibroin solution prepared in step 2 into a dialysis bag with an NWCO of 3.5KDA, dialyze with deionized water for 72 hours, change the water 7-8 times in the first 24 hours, and change the water 7-8 ti...

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Abstract

The invention provides highly-oriented silk fibroin based nanofiber which adopts silk fibroin as a base material, adopts a nerve growth factor as a functional material for inducing neuronal cell growth and is obtained by an in-phase spinning controller with special design through an electrospinning fiber technique. Compared with traditional nano preparation technologies such as freeze drying, gas foaming, self-assembling and the like, the nerve growth factor loaded highly-oriented silk fibroin nanofiber scaffold as an artificial nerve graft has the advantages of high efficiency, simple process, low cost and the like, has structural and functional controllability which is not likely to be realized with the traditional nanotechnology, and is better in forming effect. Meanwhile, a composite of a biological material and cells is directly spun into a composite of a bracket material and the cells once with a blended spinning technology, and compared with a tradition process of firstly bracket material preparation and later cell culture on the bracket material, the process is simple.

Description

technical field [0001] The present invention relates to a highly oriented nanofiber scaffold and a preparation method thereof, in particular to a kind of silk protein loaded with nerve growth factor as a raw material, which has high orientation, can induce the growth of neuron cells, and has excellent mechanical properties. A nanofiber support and a preparation method thereof belong to the field of composite materials. Background technique [0002] Nerve regeneration is a major problem that is very confusing in the theoretical research and clinical practice of the neuroscience and medical circles and has not yet found an effective solution. At present, using the basic principles and methods of tissue engineering and according to the biological characteristics of nerve regeneration, using a carrier substance with good biocompatibility as a nerve conduit to repair peripheral nerve injuries is the best way to replace the traditional autologous sural nerve, epidermal nerve and i...

Claims

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Application Information

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IPC IPC(8): A61L27/44A61L27/38A61L27/54D04H1/42D04H1/728
Inventor 王可王曙东马倩
Owner YANCHENG INST OF IND TECH
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