Method for preparing composite nanofiber tissue engineering scaffold based on graphene oxide

Abstract

The invention provides a method for preparing a composite nanofiber tissue engineering scaffold based on graphene oxide. The method includes the following steps: a step 1, dissolving silk fibroin and high-molecular polymer in a solvent with stirring till complete dissolution of the silk fibroin and the high-molecular polymer so as to acquiring a spinning solution; a step 2, performing electrostatic spinning on the spinning solution obtained from the step 1 so as to acquire a nanofiber membrane, performing steam fumigation treatment by using ethyl alcohol, and performing drying so as to acquire a silk fibroin / high-molecular polymer composite nanofiber scaffold material; and a step 3, dipping the silk fibroin / high-molecular polymer composite nanofiber scaffold material obtained from the step 2 in a graphene oxide dispersion liquid, taking out the silk fibroin / high-molecular polymer composite nanofiber scaffold material, and drying the silk fibroin / high-molecular polymer composite nanofiber scaffold material so as to acquire the composite nanofiber tissue engineering scaffold. The composite nanofiber tissue engineering scaffold is high in mechanical property, can provide biological signals for tissue growth, can promote adherence, propagation and differentiation of cells.

Description

technical field

[0001] The invention relates to a preparation method of a graphene oxide composite nanofiber tissue engineering scaffold. Background technique

[0002] In recent years, with the aging of the population and the increase in sports injuries, the defect or failure of organs and tissues is the most frequent, destructive and costly problem. Tissue regeneration and functional recovery have always been a clinical problem.

[0003] The development of tissue engineering and regenerative medicine provides an effective treatment for reconstructing or repairing human diseased tissues and organs. The involvement of scaffold materials is one of the key factors that determine the success or failure of tissue engineering. The design of scaffold materials should maximize the bionic structure and functional characteristics of human extracellular matrix (ECM), in order to effectively solve the problem of biocompatibility of materials. An ideal scaffold material not only requi...

Examples

Embodiment 1

[0044] A preparation method based on graphene oxide composite nanofiber tissue engineering scaffold, the specific steps are:

[0045] 1. Put the tussah silk in the environment of 95~100℃, 2 CO 3 The degumming solution was degummed 3 times, each time for 30 minutes, and the bath ratio was 1:50. After degumming, the tussah silk fibers are obtained and dried at 60°C. Put the tussah silk fibroin fiber in a saturated LiSCN solution at a bath ratio of 1:10, dissolve it for 70 minutes at 50°C±2°C, put the obtained tussah silk fibroin protein solution into a dialysis bag with a molecular weight cut-off of 8-10KDa, and use Dialyzed with deionized water for 3 days, and freeze-dried to obtain tussah silk fibroin.

[0046] 2. Weigh 0.125g of silk fibroin and 0.375g of high molecular polymer, dissolve them in 5ml of hexafluoroisopropanol, and magnetically stir at a certain rate until completely dissolved to obtain a spinning fiber with a concentration of 10% (w / v). liquid. Perform ele...

Embodiment 2

[0049] A preparation method based on graphene oxide composite nanofiber tissue engineering scaffold, the specific steps are:

[0050] 1. Place the tussah silk at 95~100℃ 2 CO 3 The degumming solution was degummed 3 times, each time for 30 minutes, and the bath ratio was 1:50. After degumming, the tussah silk fibers are obtained and dried at 60°C. Put the tussah silk fibroin fiber in a saturated LiSCN solution at a bath ratio of 1:10, dissolve it for 70 minutes at 50°C±2°C, put the obtained tussah silk fibroin protein solution into a dialysis bag with a molecular weight cut-off of 8-10KDa, and use Dialyzed with deionized water for 3 days, and freeze-dried to obtain tussah silk fibroin.

[0051] 2. Weigh 0.125g of silk fibroin and 0.375g of high molecular polymer, dissolve them in 5ml of hexafluoroisopropanol, and magnetically stir at a certain rate until completely dissolved to obtain a spinning fiber with a concentration of 10% (w / v). liquid. Perform electrospinning and w...

Embodiment 3

[0054] A preparation method based on graphene oxide composite nanofiber tissue engineering scaffold, the specific steps are:

[0055] 1. Put the tussah silk in the environment of 95~100℃, 2 CO 3 The degumming solution was degummed 3 times, each time for 30 minutes, and the bath ratio was 1:50. After degumming, the tussah silk fibers are obtained and dried at 60°C. Put the tussah silk fibroin fiber in a saturated LiSCN solution at a bath ratio of 1:10, dissolve it for 70 minutes at 50°C±2°C, put the obtained tussah silk fibroin protein solution into a dialysis bag with a molecular weight cut-off of 8-10KDa, and use Dialyzed with deionized water for 3 days, and freeze-dried to obtain tussah silk fibroin.

[0056] 2. Weigh 0.125g of silk fibroin and 0.375g of high molecular polymer, dissolve them in 5ml of hexafluoroisopropanol, and magnetically stir at a certain rate until completely dissolved to obtain a spinning fiber with a concentration of 10% (w / v). liquid. Perform ele...

technical field

[0001] The invention relates to a preparation method of a graphene oxide composite nanofiber tissue engineering scaffold. Background technique

[0002] In recent years, with the aging of the population and the increase in sports injuries, the defect or failure of organs and tissues is the most frequent, destructive and costly problem. Tissue regeneration and functional recovery have always been a clinical problem.

[0003] The development of tissue engineering and regenerative medicine provides an effective treatment for reconstructing or repairing human diseased tissues and organs. The involvement of scaffold materials is one of the key factors that determine the success or failure of tissue engineering. The design of scaffold materials should maximize the bionic structure and functional characteristics of human extracellular matrix (ECM), in order to effectively solve the problem of biocompatibility of materials. An ideal scaffold material not only requi...

Examples

Embodiment 1

[0044] A preparation method based on graphene oxide composite nanofiber tissue engineering scaffold, the specific steps are:

[0045] 1. Put the tussah silk in the environment of 95~100℃, 2 CO 3 The degumming solution was degummed 3 times, each time for 30 minutes, and the bath ratio was 1:50. After degumming, the tussah silk fibers are obtained and dried at 60°C. Put the tussah silk fibroin fiber in a saturated LiSCN solution at a bath ratio of 1:10, dissolve it for 70 minutes at 50°C±2°C, put the obtained tussah silk fibroin protein solution into a dialysis bag with a molecular weight cut-off of 8-10KDa, and use Dialyzed with deionized water for 3 days, and freeze-dried to obtain tussah silk fibroin.

[0046] 2. Weigh 0.125g of silk fibroin and 0.375g of high molecular polymer, dissolve them in 5ml of hexafluoroisopropanol, and magnetically stir at a certain rate until completely dissolved to obtain a spinning fiber with a concentration of 10% (w / v). liquid. Perform ele...

Embodiment 2

[0049] A preparation method based on graphene oxide composite nanofiber tissue engineering scaffold, the specific steps are:

[0050] 1. Place the tussah silk at 95~100℃ 2 CO 3 The degumming solution was degummed 3 times, each time for 30 minutes, and the bath ratio was 1:50. After degumming, the tussah silk fibers are obtained and dried at 60°C. Put the tussah silk fibroin fiber in a saturated LiSCN solution at a bath ratio of 1:10, dissolve it for 70 minutes at 50°C±2°C, put the obtained tussah silk fibroin protein solution into a dialysis bag with a molecular weight cut-off of 8-10KDa, and use Dialyzed with deionized water for 3 days, and freeze-dried to obtain tussah silk fibroin.

[0051] 2. Weigh 0.125g of silk fibroin and 0.375g of high molecular polymer, dissolve them in 5ml of hexafluoroisopropanol, and magnetically stir at a certain rate until completely dissolved to obtain a spinning fiber with a concentration of 10% (w / v). liquid. Perform electrospinning and w...

Embodiment 3

[0054] A preparation method based on graphene oxide composite nanofiber tissue engineering scaffold, the specific steps are:

[0055] 1. Put the tussah silk in the environment of 95~100℃, 2 CO 3 The degumming solution was degummed 3 times, each time for 30 minutes, and the bath ratio was 1:50. After degumming, the tussah silk fibers are obtained and dried at 60°C. Put the tussah silk fibroin fiber in a saturated LiSCN solution at a bath ratio of 1:10, dissolve it for 70 minutes at 50°C±2°C, put the obtained tussah silk fibroin protein solution into a dialysis bag with a molecular weight cut-off of 8-10KDa, and use Dialyzed with deionized water for 3 days, and freeze-dried to obtain tussah silk fibroin.

[0056] 2. Weigh 0.125g of silk fibroin and 0.375g of high molecular polymer, dissolve them in 5ml of hexafluoroisopropanol, and magnetically stir at a certain rate until completely dissolved to obtain a spinning fiber with a concentration of 10% (w / v). liquid. Perform ele...

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