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Immediately crosslinking technology for preparing macroporous three-dimensional nanofiber bracket

A three-dimensional nano-fiber scaffold technology, applied in fiber processing, prosthesis, filament/wire forming, etc., can solve problems such as complex technology

Active Publication Date: 2014-06-18
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the collection device that can obtain a fluffy structure is combined with the method of reactive electrospinning, the structure collapse caused by later cross-linking can be avoided, but the technology used in this method is relatively complicated

Method used

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  • Immediately crosslinking technology for preparing macroporous three-dimensional nanofiber bracket
  • Immediately crosslinking technology for preparing macroporous three-dimensional nanofiber bracket
  • Immediately crosslinking technology for preparing macroporous three-dimensional nanofiber bracket

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Cut silkworm cocoons into 1cm 2 The small pieces were boiled with 500mL of 0.5% sodium carbonate solution for 1.5 hours, cleaned with deionized water, boiled in 500mL of deionized water for 0.5h, washed with deionized water and dried. Add about 60mL of calcium chloride / water / ethanol three-phase solution (the molar ratio of the three substances is 1 / 8 / 2) to the treated silk, stir in a water bath at 70°C for 1 hour to completely dissolve the silk, and then add the obtained The solution was dialyzed for 48 hours. After the dialysis, the solution was centrifuged at 8000r / min for 30 minutes and then filtered. The filtered solution was frozen in liquid nitrogen and then dried for 48 hours to obtain regenerated silk fibroin.

[0035] 2. Dissolve 2 g of regenerated silk fibroin in 8 g of a mixed solution of dichloromethane and trifluoroacetic acid at a mass ratio of 3:7 to prepare a solution for electrospinning with a mass fraction of regenerated silk fibroin of 20%.

[00...

Embodiment 2

[0042] 1. Cut silkworm cocoons into 1cm 2 The small pieces were boiled with 500mL of 0.5% sodium carbonate solution for 1.5 hours, cleaned with deionized water, boiled in 500mL of deionized water for 0.5h, washed with deionized water and dried. Add about 60mL of calcium chloride / water / ethanol three-phase solution (the molar ratio of the three substances is 1 / 8 / 2) to the treated silk, stir in a water bath at 70°C for 1 hour to completely dissolve the silk, and then add the obtained The solution was dialyzed for 48 hours. After the dialysis, the solution was centrifuged at 8000r / min for 30 minutes and then filtered. The filtered solution was frozen in liquid nitrogen and then dried for 48 hours to obtain regenerated silk fibroin.

[0043] 2. Mix and dissolve 0.5g of regenerated silk fibroin and 0.5g of chitosan in 9g of a mixed solution of dichloromethane and trifluoroacetic acid with a mass ratio of 3:7 to prepare a mixture for electrospinning with a mass fraction of 10%. sol...

Embodiment 3

[0050] 1. Cut silkworm cocoons into 1cm 2 The small pieces were boiled with 500mL of 0.5% sodium carbonate solution for 1.5 hours, cleaned with deionized water, boiled in 500mL of deionized water for 0.5h, washed with deionized water and dried. Add about 60mL of calcium chloride / water / ethanol three-phase solution (the molar ratio of the three substances is 1 / 8 / 2) to the treated silk, stir in a water bath at 70°C for 1 hour to completely dissolve the silk, and then add the obtained The solution was dialyzed for 48 hours. After the dialysis, the solution was centrifuged at 8000r / min for 30 minutes and then filtered. The filtered solution was frozen in liquid nitrogen and then dried for 48 hours to obtain regenerated silk fibroin.

[0051] 2. Mix and dissolve 0.75g of regenerated silk fibroin and 0.25g of chitosan in 9g of a mixed solution of dichloromethane and trifluoroacetic acid at a mass ratio of 3:7 to prepare a mixture for electrospinning with a mass fraction of 10%. sol...

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Abstract

The invention provides a method of preparing a three-dimensional nanofiber bracket by combining an immediately crosslinking technology and an electrostatic spinning technology. The bracket prepared according to the invention is of a three dimension solid structure, the thickness of the bracket is 0.05-10mm, the interfibrous gap is 0-30 microns, and the pore size is adjustable. With the adoption of the method, the operation is simple, the relevant immediately crosslinking method solves the problems that fibers are dissolved and the structure is damaged caused by crosslinking after natural biomolecules with poorer mechanical and processing performances carry out electrostatic spinning, so that the nanofiber bracket which has a stable structure, is in three dimension solid and is loose and porous can be obtained, and a platform used for carrying cells, cell factors and drugs is prod for tissue regeneration and repair.

Description

technical field [0001] The invention belongs to the field of implantable medical devices, and in particular relates to an immediate cross-linking technology used to prepare a macroporous three-dimensional nanofiber support. Background technique [0002] Diseases and traumas often cause tissue and organ damage and dysfunction, and are one of the risk factors that threaten human health and life. At present, autologous tissue or organ transplantation is the first choice for defect repair, but the donor area of ​​this method is limited. However, there is immune rejection in allogeneic tissues and organs, and certain tissues of heterogeneous tissues are also used for repair, such as bone, but there is a risk of infection. From this foundation developed tissue engineering, the discipline devoted to finding biological substitutes to repair, maintain and improve tissues. Its three elements include seed cells, tissue engineering scaffolds and growth factors. Among them, scaffold i...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22A61L27/56D04H1/728D01D5/00
Inventor 王家伟丁慧芬
Owner WUHAN UNIV
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