Titanium net with fimbrin and its prepn and application
A technology of silk fibroin and titanium mesh, applied in biochemical equipment and methods, medical science, microbiology, etc., to reduce complications, promote tracheal mucosal repair and clinical reconstruction of tracheal defects
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Embodiment 1
[0069] Embodiment 1 prepares silk fibroin
[0070] Use waste mulberry silkworm raw silk to degumming in 100°C NaHCO3 (0.5wt%) aqueous solution for 45 minutes, and dissolve the degummed silk in 9.3mol / L LiBr aqueous solution, and keep stirring at 45°C for about 2 hours until the silk is completely dissolved . Filter with eight layers of medical gauze to remove undissolved silk and impurities. The obtained silk fibroin protein salt solution is dialyzed with deionized water for four days to remove LiBr in the solution. The cut-off molecular weight of the dialysis bag is 12,000-14,000Da. The prepared silk fibroin has a concentration of about 4%, further concentrating it, continuing to dialyze with 10% PEG for one day, centrifuging the dialyzed silk fibroin solution at 6000 rpm for 15 minutes to remove impurities, and leaving the supernatant. The obtained silk fibroin has a concentration of 20%, and it is stored in a refrigerator at 4° C. as a storage solution for future use. The...
Embodiment 2
[0071] Embodiment 2 prepares silk fibroin coated titanium mesh
[0072] Use medical titanium mesh (commercially available) to make the shape of a trachea-like ring-like structure with the same curvature and thickness as the experimental animal rabbit trachea. After ethylene oxide fumigation and sterilization, the titanium mesh is repeatedly dipped in The above-mentioned silk fibroin solution with a dilution concentration of 6% stored at low temperature is taken out and placed in an oven to dry at 60 degrees. Repeat this 6-10 times to make the surface evenly coated with silk fibroin material and keep the pores of the titanium mesh unsealed. All of the above processes were performed aseptically.
Embodiment 3
[0074] Use medical titanium mesh (commercially available) to make the shape of a trachea-like ring-like structure with the same curvature and thickness as the experimental animal rabbit trachea. After ethylene oxide fumigation and sterilization, the titanium mesh is repeatedly dipped in The above-mentioned silk fibroin solution with a dilution concentration of 12% stored at low temperature is taken out and placed in an oven to dry at 60 degrees. Repeat this 6-8 times to make the surface evenly coated with silk fibroin material and keep the pores of the titanium mesh unsealed. All of the above processes were performed aseptically.
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