Method for building three-dimensional skin model applied to anti-inflammatory and anti-allergy effect evaluation

A technology for skin model and method establishment, applied in the direction of epidermal cells/skin cells, biochemical equipment and methods, preparations for in vivo tests, etc., can solve the inconvenience, high cost, and difficulty in fully reflecting the sensitization and inflammation process and other problems, to achieve the effect of excellent application prospect and simple preparation method

Active Publication Date: 2017-10-20
WEST CHINA HOSPITAL SICHUAN UNIV
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Problems solved by technology

At present, it is very inconvenient and costly to screen anti-inflammatory and anti-allergic drugs or cosmetic raw materials for skin, ear swelling of mice induced by xylene, swelling of paws of rats induced by egg white, chronic inflammation model of cotton ball granuloma in rats, small Rat local lymph node sensitization experiment is a commonly used animal experimental model
However, with European cosmetics regulations promulgating

Method used

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  • Method for building three-dimensional skin model applied to anti-inflammatory and anti-allergy effect evaluation
  • Method for building three-dimensional skin model applied to anti-inflammatory and anti-allergy effect evaluation
  • Method for building three-dimensional skin model applied to anti-inflammatory and anti-allergy effect evaluation

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Embodiment 1

[0046] Embodiment 1 Preparation of three-dimensional skin model of the present invention

[0047] In vitro, the whole layer of skin including all types of cells of immune cells (lymphocytes, dendritic cells, granulocytes, monocytes, macrophages, etc.) and biological scaffold materials are combined to construct a skin with a structure similar to that of normal skin. method.

[0048] 1 The construction method of the three-dimensional skin model of the present invention

[0049] 1.1 Source and preparation of various cells

[0050] 1) Obtaining of fibroblasts and keratinocytes:

[0051] The skin tissue of a normal person was taken, the tissue was sterilized, and small pieces (3mm×3mm) of dermis tissue and epidermis tissue were separated. The tissue pieces were placed in a sterile petri dish, and culture medium (DMEM medium containing 10% FBS) was added. Small pieces of tissue adhere to the surface of the culture dish, and some cells will grow on the surface of the culture dish...

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Abstract

The invention provides a method for building a three-dimensional skin model. The method comprises the following steps: 1) preparing an extracellular matrix polymeric hydrogel solution; 2) mixing fibroblast and the extracellular matrix polymeric hydrogel solution, and culturing through a small culture chamber; and 3) inoculating cutin to form cells; performing air-liquid interface culture to obtain the three-dimensional skin model containing a corium layer, an epidermal layer and a cutin layer. The method is capable of effectively building the three-dimensional skin model; in addition, the preparation method is simple and convenient, and can be applied to the sieving of anti-inflammatory and anti-allergy skin drugs; and the method has a good application prospect.

Description

technical field [0001] The present invention relates to a method for establishing a skin model, in particular to a method for establishing a novel three-dimensional skin model for evaluating anti-inflammatory and anti-allergic effects. Background technique [0002] As an important tissue and organ that covers and protects the body surface, the skin is the first barrier to block the interference of external substances. It is easily damaged by factors such as trauma, burns, inflammation, ulcers, postoperative tumors, and congenital diseases. At the same time, it will pass some special reactions to defend. The incidence of allergic skin diseases such as dermatitis and eczema in the whole world exceeds 10%. At present, it is very inconvenient and costly to screen anti-inflammatory and anti-allergic drugs or cosmetic raw materials for skin, ear swelling of mice induced by xylene, swelling of paws of rats induced by egg white, chronic inflammation model of cotton ball granuloma i...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/077C12N5/0783C12N5/0784C12N5/0786C12N5/0787A61K49/00
CPCA61K49/0008C12N5/0629C12N5/0636C12N5/0639C12N5/0642C12N5/0645C12N5/0656C12N2513/00C12N2533/30C12N2533/54C12N2533/80
Inventor 熊丽丹唐洁华薇周蓉颖李利
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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