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Tissue engineering composite skin material, and its preparing method

A tissue engineering and skin technology, applied in the field of tissue engineering composite skin materials and its preparation, can solve the problems of missed transplantation period and long time of culturing cells, and achieve the effect of fast transplantation, good wound protection and more normal appearance

Inactive Publication Date: 2007-07-25
北京赛尔泰和生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The autologous composite skin developed by American companies and Han Bin in my country has a good effect on wound healing. However, due to the long time of culturing cells, the transplantation can only be started about 2 weeks after skin injury or burn, which is often missed. best transplant period

Method used

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  • Tissue engineering composite skin material, and its preparing method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, preparation of tissue engineering composite skin material

[0033] 1. Extraction of patient's own healthy cells and cell culture of healthy neonatal foreskin

[0034] (1) Extraction of cells

[0035] 1. Materials obtained from the operating room

[0036] 1) Use tincture of medical iodine to clean the patient's skin surface;

[0037] 2) Rinse the leather surface with physiological saline to remove residual iodine tincture;

[0038] 3) Take a narrow and long skin of 1cm×2.5cm;

[0039] 4) Put the removed skin into the storage solution and send it to the cell culture room.

[0040] 2. Preservation of skin tissue after collection: use 5 ml of DMEM (purchased from Hyclone, USA) culture solution containing 100 units / ml penicillin and 100 mg / ml streptomycin to preserve the tissue in a preservation tube (4° C.).

[0041] 3. Sterilize and clean in an ultra-clean workbench, and separate large pieces of tissue: wash the tissue with 0.1M PBS containing 100 units / ...

Embodiment 2

[0080] Embodiment 2, clinical report

[0081] 1. Inclusion criteria

[0082] 1. The wounds of patients with deep burns after incision and scab removal.

[0083] 2. Long-term non-healing ulcer wounds and granulation wounds caused by various reasons (late granulation wounds after third-degree burns, refractory ulcer wounds such as diabetic feet, etc.).

[0084] 3. Extensive scar hyperplasia, contracture deformity, or keloids, hypertrophic scars, hard, itchy, painful scars all over the body, scar removal and skin grafting are required.

[0085] Patients who belong to the above situation and are under the age of 60 are eligible for inclusion.

[0086] 2. Criteria for Excluding Cases

[0087] 1. Compound injuries such as shock period and chemical poisoning.

[0088] 2. Combined with serious primary diseases such as cardiovascular, cerebrovascular, liver, kidney, and hematopoietic system.

[0089] 3. People with allergies or autoimmune diseases.

[0090] 3. Materials

[0091]...

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Abstract

A composite skin material prepared by tissue engineering for skin implantation is composed of the hypoderminal cell layer and epithelial cell layer attached on fibrin scaffold. Its preparing process includes such steps as taking fibroblasts and epithelial stem cells from patient itself and foreign person, dispersing them in fibrinogen liquid, preparing serozyme solution, mixing them together, and coating the mixture on the surface of wound.

Description

technical field [0001] The invention relates to a tissue engineering composite skin material and a preparation method thereof, belonging to the field of tissue engineering. Background technique [0002] Tissue engineering technology is a high-tech technology established in the world after 1980 for the preparation of transplantable tissues and organs with morphological characteristics and functions. The products currently being developed internationally include: skin, dermis , mucous membranes, tendons, bones, cartilage, blood vessels, nerves, cornea, heart valves, kidneys, glands and digestive organs, etc., and the skin tissue, dermis tissue and cartilage tissue were the first to succeed. Skin and dermal tissue were the first products to receive FDA approval in 1999, including Apligraf, Integraf, and Dermagraf. These products all use allogeneic skin sources to culture epithelial and dermal cells in vitro. Since allogeneic skin tissue is derived from the foreskin of newborn...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/60
Inventor 韩斌
Owner 北京赛尔泰和生物医药科技有限公司
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