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Centella asiatica triterpenic acid single-glucopyranoside composition, its preparation method, its quantitative analysis method and its application

A technology for monoglucoside and triterpene acid, which is applied to the preparation of a composition of centella asiatica triterpene acid monoglucoside by biotransforming centella asiatica extract, and the field of centella asiatica triterpene acid monoglucoside composition, It can solve the problems such as the report on the quality standard research and use of the triterpenoid monoglucoside composition of Centella asiatica

Inactive Publication Date: 2012-02-15
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0025] So far, there have been no reports on the composition of Centella asiatica triterpene acid monoglucoside and its preparation process research, quality standard research and use

Method used

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  • Centella asiatica triterpenic acid single-glucopyranoside composition, its preparation method, its quantitative analysis method and its application
  • Centella asiatica triterpenic acid single-glucopyranoside composition, its preparation method, its quantitative analysis method and its application
  • Centella asiatica triterpenic acid single-glucopyranoside composition, its preparation method, its quantitative analysis method and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) Take by weighing 1kg Centella asiatica water extract, add water to 5kg;

[0059] (2) Add 1 g of Aspergillus niger β-glucosidase, hydrolyze at 40° C. for 72 hours;

[0060] (3) Filtrate and separate the enzymolysis solution, concentrate under reduced pressure to 2L, and extract once with 6L water-saturated n-butanol to obtain n-butanol extract; Centella triterpene acid monoglucoside composition.

[0061] Adopt containing 0.1mmol / Lβ-cyclodextrin, and the aqueous solution that volume percent is 15% acetonitrile is used as mobile phase to carry out HPLC measurement, in the result composition chebuside II, hydroxy asiatic acid monoglucoside, asiatic acid monoglucoside The mass percentages of the three are respectively 14.8%, 23.5%, and 26.4%, and the sum of the three mass percentages is 64.7%.

Embodiment 2

[0063] (1) Take by weighing 1kg of asiatica alcohol extract, add water to 40Lkg;

[0064] (2) Add 50 g of laetrile, and hydrolyze at 20° C. for 24 hours;

[0065] (3) centrifuge the enzymolysis solution, concentrate it under reduced pressure to 4L, and extract it three times with 4L water-saturated n-butanol to obtain the n-butanol extract; Centella triterpene acid monoglucoside composition.

[0066] Adopt containing 1.0mmol / Lβ-cyclodextrin, and the aqueous solution that volume percent is 50% acetonitrile is used as mobile phase to carry out HPLC measurement, and in the result composition The mass percentages of the three are respectively 18.2%, 28.1%, and 27.4%, and the sum of the three mass percentages is 73.7%.

Embodiment 3

[0068] (1) Take by weighing 1kg of Centella asiatica total saponins, add water to 1000kg,

[0069] (2) Add 0.5kg of Penicillium expansica β-glucosidase, hydrolyze at 60°C for 0.5 hour,

[0070] (3) Filtration and separation of the enzymolysis solution, adsorption on a 10L macroporous resin column of type D101, washing with water to remove impurities, and eluting with 40L of 60% volume concentration ethanol solution to obtain an ethanol eluent. The ethanol eluent is concentrated under reduced pressure and dried in vacuum to obtain the centella asiatica triterpene acid monoglucoside composition.

[0071] Using 0.4mmol / L beta-cyclodextrin, and 30% by volume aqueous solution of methanol as the mobile phase for HPLC determination, the results of the composition of cebuside II, hydroxy asiatic acid monoglucoside, asiatic acid monoglucoside The mass percentages of the three are respectively 22.0%, 31.5%, and 29.7%, and the sum of the three mass percentages is 83.2%.

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Abstract

The invention relates to a centella asiatica triterpenic acid single-glucopyranoside composition which is composed of ursane madecassic acid single-glucopyranoside, madecassic acid single-glucopyranoside and oleanane chebuloside II, wherein the mass ratio of ursane madecassic acid single-glucopyranoside to madecassic acid single-glucopyranoside to oleanane chebuloside II is 1:0.5-2:0.1-1, and the sum of the mass percentage content of three components is not less than 50%. The composition takes a centella asiatica extract as a substrate, the centella asiatica extract is fermented and hydrolyzed by microbes of beta-glucosidase or microbes capable of generating beta-glucosidase, and extracted by n-butanol or separated and purified by macroporous adsorption resin. The invention also provides a quantitative analysis method which is a HPLC quantitative analysis for three components by adding a proper amount of mobile phase of beta-cyclodextrin. Experimental research of pharmacodynamics proves that the composition has substantial activity for inhibiting tumor cells and fibroblast, the composition can be used for treating tumor and scar hyperplasia.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a centella asiatica triterpene acid monoglucoside composition. In addition, the present invention also provides a preparation method for preparing a centella asiatica triterpene acid monoglucoside composition by biotransformation of centella asiatica extract, and a quantitative analysis method and application of the composition. Background technique [0002] Centella asiatica (L.) Urb, also known as Luodeda, Bengdawan, and Banbianqian, is a plant of the genus Centella asiatica in the family Umbelliferae. It was first recorded in "Shen Nong's Materia Medica" and listed as a middle grade. It is cold in nature, bitter and pungent in taste, and has the effects of clearing heat and promoting dampness, detoxifying and reducing swelling. It is mainly used for damp-heat jaundice, heatstroke diarrhea, sandy and bloody stranguria, carbuncle sores, and traumatic injuries. Centella...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P17/02A61P35/00G01N30/02
Inventor 茅仁刚宋纯清袁萍叶晓平杜鹏司鹏鹏吴博
Owner SHANGHAI NORMAL UNIVERSITY
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