Automation augmentation and culture system of induced pluripotent stem cells

A technology of pluripotent stem cells and culture system, which is applied in the field of automatic expansion and culture system of induced pluripotent stem cells, and can solve problems such as inability to meet medical needs

Active Publication Date: 2011-09-07
广东朗源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] With the massive increase of chronic and degenerative diseases, tradi...

Method used

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  • Automation augmentation and culture system of induced pluripotent stem cells
  • Automation augmentation and culture system of induced pluripotent stem cells
  • Automation augmentation and culture system of induced pluripotent stem cells

Examples

Experimental program
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Embodiment 1

[0066] Example 1: Preparation of an automated expansion and culture system for induced pluripotent stem cells of the present invention

[0067] The induced pluripotent stem cell automatic expansion and culture system of the present invention is composed of an automatic cell expansion and culture system, an operation room, a liquid transmission system and a control system.

[0068] 1. Automatic cell expansion system: including air purification and introduction system, circular rotary incubator, incubator digital power system, digital temperature control system, sterilization system, automatic opening and closing system of the petri dish cover, automatic ejection and closing of the petri dish system, automatic door control system and sensors.

[0069] The automatic cell expansion system is divided into two layers in structure, the upper layer is the culture device, and the lower layer is the power system and control system and other devices, such as figure 1 and figure 2 As s...

Embodiment 2

[0077] Embodiment 2: Application of the present invention to carry out automatic amplification and cultivation of 293T

[0078] Placement of the culture plate and information input: Use the external barcode scanning head of the USB interface to input the barcode information on the 12-well culture dish, and then place it in the corresponding fixed block 2.3 of the culture plate rack 2, and the system will automatically determine the value of the culture plate. Put address information. Set the temperature on the human dialogue operation panel 19 of the incubator to be 37°C, humidity 100% and CO 2 Concentration 5%.

[0079] Automated induction of stem cells: 1. Firstly, the 293T packaging cells were artificially cultured in separate trays. After the 293T packaging cells were recovered and passaged for more than 3 times, the medium in the culture dish was washed, washed once with 3-5ml PBS, and digested with 0.25% trypsin in 600??l for 30s. Then add 293T medium to stop the dige...

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Abstract

The invention relates to the technical field of cell automation augmentation and culture instrument, in particular relates to an automation augmentation and culture system of induced pluripotent stem cells, which comprises a cell automation augmentation and culture system, an operation room and a control system which are mutually connected, wherein, the cell automation augmentation and culture system comprises a culture box and a culture box control room connected with the culture box; the culture box comprises at least one culture device for fixing a culture container fixed block of a culture container, a culture container cover automatic opening and closing system, a culture container automatic popping and closing system, a digital temperature system and at least one sensor; the culture box control room comprises an air flow purification and induction system and a culture box digital power system; and the operation room comprises a bar code automatic entry system, an liquid automatic replace system and a cell on-line observing system. By utilizing the system provided by the invention, a somatic cell can be automatically induced into a pluripotent stem cell, and the system can be used for stem cell research and clinical stem cell treatment.

Description

technical field [0001] The patent of the present invention relates to the related technical field of automatic cell expansion and culture equipment, in particular, an induced pluripotent stem cell automatic expansion and culture system. Background technique [0002] With the massive increase of chronic and degenerative diseases, traditional medicine and surgical treatment can no longer meet the future medical needs. The rise of regenerative medicine based on stem cell technology is expected to bring about the third medical revolution and provide new solutions for human health needs. The rapid development of stem cells and related reprogramming technology has promoted the development of stem cell technology. The new somatic cell reprogramming technology is of great significance to the clinical application of stem cells and the future development of related technologies, and has become a hot spot in the field of stem cell regenerative medicine research. The optimization of r...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/34
CPCC12M23/50C12M23/34C12M41/48C12M23/48C12M29/06C12M23/38C12M41/14C12M29/26C12M41/36C12M23/10
Inventor 裴端卿曾令文熊业华方志远列浦昌
Owner 广东朗源生物科技有限公司
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