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A pf‑127‑lv‑nfcocktail complex loaded with transplanted neural stem cells and its preparation method and application

A technology of pf-127-lv-nfcocktail and neural stem cells, which is applied in the biological field to achieve the effects of increasing proliferation, promoting nerve growth, and increasing the ratio of differentiation

Active Publication Date: 2017-09-15
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Another obstacle to neural stem cell transplantation is that after the transplanted neural stem cells treated with trypsin are directly transplanted into the spinal cord transection injury in the form of cell suspension, most of them can only survive on the edge of the transection lesion and cannot evenly fill the gap of the transection injury, resulting in post-differentiation. Neurons' axon extensions only partially bridge the damaged area

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  • A pf‑127‑lv‑nfcocktail complex loaded with transplanted neural stem cells and its preparation method and application

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Embodiment 1

[0046] A PF-127-LV-NFcocktail complex loaded with transplanted neural stem cells, which includes the following components: Pluronic F-127 hydrogel, complete neural stem cell medium, Lingo-1 shRNA lentiviral expression vector and nerve growth factor mixture;

[0047] Among them, the nerve growth factor mixture is composed of the following components: brain-derived neurotrophic factor, neurotrophic factor-3, platelet-derived growth factor, insulin-like growth factor 1, epidermal growth factor, basic fibroblast growth factor and glue cytoplasmic cell-derived neurotrophic factor.

[0048] In this embodiment, the mixing ratio of Pluronic F-127 hydrogel and complete neural stem cell culture medium is to add 20 g of Pluronic F-127 hydrogel to every 100 mL of complete neural stem cell culture medium;

[0049] In this embodiment, the mass ratio of Pluronic F-127 hydrogel to nerve growth factor mixture is 20:15;

[0050] In this example, the titer of the Lingo-1 shRNA lentiviral expre...

Embodiment 2

[0065] A PF-127-LV-NFcocktail complex loaded with transplanted neural stem cells, which includes the following components: Pluronic F-127 hydrogel, complete neural stem cell medium, Lingo-1 shRNA lentiviral expression vector and nerve growth factor mixture;

[0066]Among them, the nerve growth factor mixture is composed of the following components: brain-derived neurotrophic factor, neurotrophic factor-3, platelet-derived growth factor, insulin-like growth factor 1, epidermal growth factor, basic fibroblast growth factor and glue cytoplasmic cell-derived neurotrophic factor.

[0067] In this embodiment, the mixing ratio of Pluronic F-127 hydrogel and complete neural stem cell medium is to add 15 g of Pluronic F-127 hydrogel per 100 mL of complete neural stem cell medium;

[0068] In this embodiment, the mass ratio of Pluronic F-127 hydrogel to nerve growth factor mixture is 15:10;

[0069] In this example, the titer of the Lingo-1 shRNA lentiviral expression vector in the mi...

Embodiment 3

[0084] A PF-127-LV-NFcocktail complex loaded with transplanted neural stem cells, which includes the following components: Pluronic F-127 hydrogel, complete neural stem cell medium, Lingo-1 shRNA lentiviral expression vector and nerve growth factor mixture;

[0085] Among them, the nerve growth factor mixture is composed of the following components: brain-derived neurotrophic factor, neurotrophic factor-3, platelet-derived growth factor, insulin-like growth factor 1, epidermal growth factor, basic fibroblast growth factor and glue cytoplasmic cell-derived neurotrophic factor.

[0086] In this embodiment, the mixing ratio of Pluronic F-127 hydrogel and complete neural stem cell medium is to add 25 g of Pluronic F-127 hydrogel to every 100 mL of complete neural stem cell medium;

[0087] In this embodiment, the mass ratio of Pluronic F-127 hydrogel to nerve growth factor mixture is 25:20;

[0088] In this example, the titer of the Lingo-1 shRNA lentiviral expression vector in ...

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Abstract

The invention relates to the field of biotechnology and in particular relates to a PF-127-LV-NFcocktail compound loaded with portable neural stem cells as well as a preparation method and application of the PF-127-LV-NFcocktail compound. The compound comprises Pluronic F-127 hydrogel, a complete neural stem cell culture medium, a Lingo-1 shRNA slow virus expression vector and a nerve growth factor mixture. The preparation method of the PF-127-LV-NFcocktail compound comprises the following steps: 1, dissolving the Pluronic F-127 hydrogel in the complete neural stem cell culture medium; 2, adding the Lingo-1 shRNA slow virus expression vector; 3, adding the nerve growth factor mixture; and 4, performing filtration and sterilization. The compound can load the portable neural stem cells and can promote directional differentiation and nerve regeneration of the neural stem cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a PF-127-LV-NFcocktail complex loaded with transplanted neural stem cells and its preparation method and application. Background technique [0002] The treatment for the acute stage of spinal cord injury is mainly hormone and traditional surgical treatment, but these treatments have no obvious effect on various neurological dysfunctions secondary to spinal cord injury. The occurrence and development of spinal cord injury are often related to the death of nerve cells after injury and the progressive atrophy of the associated projection neurons. The symptoms are mainly due to the loss of a large number of nerve cells due to injury, and the disruption of neural network connections resulting in interruption of neurotransmission. the result of. Even if there is a certain degree of axon regeneration after spinal cord injury, its regeneration ability is extremely limited. More and more evi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A61L27/36A61L27/22A61L27/26A61L27/18A61L27/52A61L27/54
Inventor 吴洪福吴武田邱文锋熊兴东周光纪崔新月陈立毅张文辉罗传铭廖小龙
Owner GUANGDONG MEDICAL UNIV
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