Method for adjusting and controlling in vitro three-dimensional directed differentiation of stem cells

A technology of directional differentiation and stem cells, which is applied in the field of regenerative medicine, can solve the problems of unfavorable cell culture and tissue repair of degradation products, lack of special extracellular matrix components that promote differentiation, and cells do not have normal physiological functions in vivo, so as to achieve induction efficiency and Improved cell function, low cost, improved differentiation efficiency and functional effects
CN104046587AInactive Publication Date: 2014-09-17DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2014-09-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of regenerative medicine and discloses a method for adjusting and controlling in vitro three-dimensional directed differentiation of stem cells. The method includes following steps: embedding Stem cells into calcium alginate micro gel beads containing chondroitin sulfate, adding an inductive differentiation culture liquid, adjusting and controlling the in vitro directed differentiation of the stem cells by changing a mass ratio between the sodium alginate and the chondroitin sulfate during the preparation of the micro gel beads, dissolving the calcium alginate micro gel beads in a sodium citrate solution after the differentiation process being finished, and then obtaining pure differentiation cells. The method is simple in operation and low in cost, the calcium alginate micro gel beads containing chondroitin sulfate can simulate an in vivo three-dimensional extracellular matrix so that a three-dimensional directed differentiating microenvironment which approximates an in vivo microenvironment is supplied for the stem cells, thereby improving directed differentiation efficiency and a biological function of the stem cells. The method plays an important role in applications of regenerative medicine.
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Description

technical field

[0001] The invention relates to the field of regenerative medicine, and is a method for regulating three-dimensional directional differentiation of stem cells in vitro. Background technique

[0002] Stem cells are ideal seed cells for repairing damaged tissues due to their ability to self-renew and differentiate. However, when stem cells are implanted in the damaged part of the body, in addition to differentiating into the required tissue cells and participating in tissue repair, the stem cells also differentiate into other types of cells, and even have high tumorigenicity. Pre-differentiation of stem cells in vitro, so that they can be pre-differentiated into mature tissue cells or precursor cells before transplantation, can improve the efficiency of directed differentiation, reduce the ability of stem cells to differentiate into other types of cells in vivo, thereby improving the effect of tissue repair and reducing Tumor risk.

[0003] In vivo cells grow...

Claims

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