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Preparation method and application of tissue engineering material for promoting directional differentiation of mesenchymal stem cells

A bone marrow mesenchymal and tissue engineering technology, applied in the field of biomedical engineering, can solve the problems of inability to produce insulin-secreting cells, low differentiation efficiency, and short lifespan of insulin-secreting cells.

Inactive Publication Date: 2016-06-15
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still several major problems in the current directed differentiation of stem cells: first, the differentiation efficiency is low, and a sufficient number of insulin-secreting cells cannot be produced; second, the lifespan of insulin-secreting cells induced to differentiate is short; Hyperglucose responsiveness, that is, when blood glucose rises, insulin-secreting cells cannot respond to increase insulin secretion
[0005] At present, there are few domestic and foreign literature reports on the combination of stem cells and biomaterials to induce differentiation into insulin-secreting cells

Method used

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  • Preparation method and application of tissue engineering material for promoting directional differentiation of mesenchymal stem cells
  • Preparation method and application of tissue engineering material for promoting directional differentiation of mesenchymal stem cells
  • Preparation method and application of tissue engineering material for promoting directional differentiation of mesenchymal stem cells

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1: the preparation of porous PLGA scaffold

[0040] Dissolve 0.5g of PLGA (molecular weight 15KD) (PLA:PGA is 85:15, purchased from Shandong Provincial Institute of Medical Devices) raw materials in 1,4-dioxane solution, and prepare a PLGA concentration of 10% (w / v) solution. Add NaCl particles with a particle size of 150-200 μm and a ratio of 50% (w / w) to the above-mentioned polylactic-glycolic acid solution with a concentration of 10% w / v, and stir to obtain a uniform suspension. ×8cm petri dish. Drying at room temperature for 48h, freeze-drying for 48h. After demolding, use distilled water to filter out the NaCl in the porous film, and dry it at room temperature for 48 hours. Stir the deionized water in the beaker to accelerate the dissolution of NaCl particles. After the NaCl particles are completely dissolved, dry the film and seal it for storage.

Embodiment 2

[0041] Embodiment 2: the preparation of porous PLGA scaffold

[0042] Dissolve 0.5g of PLGA (molecular weight 15KD) (PLA:PGA is 85:15, purchased from Shandong Provincial Institute of Medical Devices) raw materials in 1,4-dioxane solution, and prepare a PLGA concentration of 10% (w / v) solution. Add NaCl particles with a particle size of 150-200 μm and a ratio of 25% (w / w) to the above-mentioned polylactic-glycolic acid solution with a concentration of 10% w / v, and stir to obtain a uniform suspension. ×8cm petri dish. Drying at room temperature for 48h, freeze-drying for 48h. After demolding, use distilled water to filter out the NaCl in the porous film, and dry it at room temperature for 48 hours. Stir the deionized water in the beaker to accelerate the dissolution of NaCl particles. After the NaCl particles are completely dissolved, dry the film and seal it for storage. The difference between this example and Example 1 is that the ratio of NaCl particles added during the pr...

Embodiment 3

[0043] Embodiment 3: the preparation of porous PLGA scaffold

[0044] Dissolve 0.5g of PLGA (molecular weight 15KD) (PLA:PGA is 85:15, purchased from Shandong Provincial Institute of Medical Devices) raw materials in 1,4-dioxane solution, and prepare a PLGA concentration of 10% (w / v) solution. Add NaCl particles with a particle size of 150-200 μm and a ratio of 75% (w / w) to the above-mentioned polylactic-glycolic acid solution with a concentration of 10% w / v, and stir to obtain a uniform suspension. ×8cm petri dish. Drying at room temperature for 48h, freeze-drying for 48h. After demolding, use distilled water to filter out the NaCl in the porous film, and dry it at room temperature for 48 hours. Stir the deionized water in the beaker to accelerate the dissolution of NaCl particles. After the NaCl particles are completely dissolved, dry the film and seal it for storage. The difference between this example and Example 1 is that the ratio of NaCl particles added during the pr...

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Abstract

The invention relates to the technical field of biomedical engineering, in particular to a preparation method and application of a tissue engineering material for promoting directional differentiation of mesenchymal stem cells. The preparation method provided by the invention comprises the following steps: transplanting the mesenchymal stem cells to a PLGA (poly(lactic-co-glycolic acid)) stent, by controlling the time, concentration and mode of planting the mesenchymal stem cells as well as the size, the shape and the like of a stent material, searching out optimum conditions for the directional differentiation of the mesenchymal stem cells, and observing biocompatibility of the mesenchymal stem cells and the PLGA stent, change in cellular morphology during inducing, influence of a pancreatic beta cell inducing liquid on cell proliferation as well as acquisition of specific genes, proteins and functions inducing cell differentiation. According to the preparation method disclosed by the invention, the mesenchymal stem cells are planted on the PLGA stent under the optimum conditions, and a pancreatic beta cell, which has high glucose reactivity and is applicable to therapy transplantation, is obtained from culture and differentiation by virtue of the pancreatic beta cell inducing liquid; therefore, the preparation method and application bring hope to the treatment of diabetes.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a method for preparing a tissue engineering material for promoting directional differentiation of bone marrow mesenchymal stem cells, and an application thereof. Background technique [0002] Diabetes is one of the main chronic diseases that endanger human physical and mental health. Reconstructing the function of functional islet cells in diabetic patients is an ideal goal for the treatment of diabetes. There are three main ways to restore islet function in diabetic patients: pancreas transplantation, islet transplantation and stem cell transplantation. Due to lack of donor sources and post-transplantation rejection, the clinical application of pancreas transplantation and islet transplantation is limited, making stem cell transplantation the focus of research on alternative therapies. Bone marrow mesenchymal stem cells have become an ideal cell source for stem c...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/071
Inventor 石勇铨刘浩琪汤玮
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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