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A kind of preparation method of tissue engineering scaffold with biological activity

A tissue engineering scaffold and biological activity technology, applied in the field of tissue engineering scaffold preparation, can solve the problems of scaffold biocompatibility, lack of biological activity of scaffold, inability to regulate cell behavior, etc., and achieve safe use, uniform protein properties and distribution. uniform effect

Active Publication Date: 2017-01-11
广东省医疗器械质量监督检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage is that the carbodiimide hydrochloride (EDC) and N-succinimide (NHS) used to activate the carboxyl group have certain cytotoxicity, and if they cannot be completely removed, it will affect the biocompatibility of the scaffold. Influence; the prepared scaffold lacks biological activity and cannot regulate cell behavior. In addition, this method does not describe the specific performance of the scaffold in detail

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of a bioactive tissue engineering scaffold based on soybean protein, comprising the following preparation steps:

[0032] S1. Heat treatment: disperse soybean protein in water to form a suspension with a mass concentration of 1%, stir at a constant temperature of 80°C for 0.5h, and remove insoluble components by suction filtration, and freeze the filtrate at -20°C for 12h, Freeze-drying in a dryer for 12 hours to obtain heat-treated soybean protein;

[0033] S2. Cross-linking of oxidized sodium alginate: dissolve the heat-treated soybean protein in a calcium chloride solution with a concentration of 0.2% to prepare a solution with a concentration of 8%, and then stir the prepared solution at a stirring rate of 1000rpm to make It is fully foamed, and while maintaining the same stirring rate, dropwise add a solution of 0.5% oxidized sodium alginate and continue to react for 1 hour to form a porous gel through crosslinking. The oxidation degree of the ...

Embodiment 2

[0036] A preparation method of a bioactive tissue engineering scaffold based on soybean protein, comprising the following preparation steps:

[0037] S1. Heat treatment: disperse soybean protein in water to form a suspension with a mass concentration of 5%, stir at a constant temperature of 120°C for 2 hours, remove insoluble components by suction filtration, freeze the filtrate at -4°C for 24 hours, and then freeze-dry Freeze-drying in the machine for 24 hours to obtain heat-treated soybean protein;

[0038]S2. Oxidized sodium alginate cross-linking: dissolve the heat-treated soybean protein in a calcium chloride solution with a concentration of 1% to prepare a solution with a concentration of 15%, and then stir the prepared solution at a stirring rate of 3000rpm to make It is fully foamed, and while maintaining the same stirring rate, dropwise add a 2% oxidized sodium alginate solution and continue to react for 2 hours, forming a porous gel through crosslinking, and the oxid...

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PUM

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Abstract

The invention discloses a preparation method of a tissue engineering scaffold with bioactivity. The preparation method comprises the following steps: crosslinking thermally treated soybean proteins by using oxidized sodium alginate under the condition of high speed stirring; and freezing and drying to obtain the soybean protein-based tissue engineering scaffold with bioactivity. The porous bracket prepared by the method has a relatively great bore diameter and porosity and good biocompatibility, has certain bioactivity, can be used for promoting secretion of cellular matrixes and directional differentiation of stem cells and can be used as an ideal carrier of the cell in the tissue engineering field.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a preparation method of a biologically active tissue engineering scaffold with soybean protein as the main raw material. Background technique [0002] Tissue engineering scaffolds are materials that can be combined with tissue living cells and implanted into organisms. To a certain extent, they are temporary substitutes for extracellular matrix and the most basic structure of tissue engineered tissues. Animal-derived proteins represented by collagen, silk fibroin, and keratin have been widely used in tissue engineering due to their excellent biocompatibility and biodegradability. However, animal proteins also have obvious defects as scaffold materials, including large differences in the properties of proteins from different sources, poor wet strength, and certain immunogenicity, especially the risk of carrying animal viruses. For example, collagen extracted from bovin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/56A61L27/58
Inventor 徐红蕾田冶何晓帆田小俊
Owner 广东省医疗器械质量监督检验所
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