A modified bone repair scaffold material with amino-icariin-carboxymethyl chitosan

Abstract

A novel PHBV modified induced type bone repair scaffold material is prepared by the steps: dissolving amino acetic acid in a sodium bicarbonate solution, to obtain a first solution; dissolving di-tert-butyl dicarbonate in dioxane, to obtain a second solution; dropwise adding the second solution into the first solution; adjusting the pH value to neutral, dissolving the solids, dropwise adding the filtrate into cold ethyl ether, washing and drying an insoluble substance, and thus obtaining dicarbonate t-butyloxycarboryl-glycine; carrying out condensation on icariin and dicarbonate t-butyloxycarboryl-glycine, adding a mixed liquid of trifluoroacetic acid and dichloromethane into the product, and after the reaction is stopped, evaporating; dissolving the remainder with ethyl acetate; removing the solvent, to obtain aminated icariin; carrying out a condensation reaction; pouring aminated icariin-PHBV into a chitosan solution, injecting into an annular mold, freezing with liquid nitrogen, demoulding, drying, and thus obtaining the novel PHBV modified induced type bone repair scaffold material. The bone repair scaffold material can promote directional differentiation of stem cells into osteoblasts, accelerates bone growth and functional recovery, and can effectively repair bone defects.

Description

technical field [0001] The invention belongs to the field of medical technology, and relates to a series of polyhydroxybutyrate cohydroxyvalerate (PHBV) modified inducible bone repair scaffold materials. Sex and other methods are made. Background technique [0002] Bone injury is a common clinical disease, and bone is a natural composite material with complex hierarchical structure of inorganic and organic matter. The number of fractures, bone defects and bone loss caused by wars, traffic accidents, industrial injuries, sports trauma, diseases and natural disasters reaches millions every year in the world. The damaged bones can be repaired with endogenous or exogenous bone. Endogenous bone is easy to be accepted by patients, and the repair effect is better, but its quantity is very limited, and a second operation is required, which may cause other damage; while the use of exogenous bone is easy to obtain, but the repair effect is far inferior to that of endogenous bone. At...

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Problems solved by technology

Endogenous bone is easy to be accepted by patients, and the repair effect is better, but its quantity is very limited, and a second operation is required, which may cause other damage; while the use of exogenous bone is easy to obtain, but the repair effect is far inferior to that of endogenous bone. , the current exogenous bone repair methods have immune rejection, are difficult to degrade, and may contain potential pathogens and other side effects, etc.

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