4results about How to "Functionality is not affected" patented technology

The application discloses a method for removing endotoxin from gelatin. The method comprises the following steps: (1) adding pyrogen-free water to gelatin raw materials, swelling, and then heating to 50-70 DEG C to prepare a gelatin solution with a mass concentration of 3-20%; (2) adding an adsorbent with a mass of 2.5%-100% of the gelatin, adsorbing at 40-70 DEG C for 30-240 min, and then separating the adsorbent from the gelatin solution by filtration or centrifugal separation to obtain a filtrate; (3) further filtering the filtrate by filter paper and thin-layer diatomite to obtain a refined filtrate, diluting the refined filtrate with 4 times the volume of water, ultrafiltering by using a membrane with a molecular weight cut-off of 5000-20000 Da, finally concentrating to a concentration of 10-25%, sterilizing, and drying to obtain gelatin solid with low endotoxin; the adsorbent used in step (2) is an adsorbent with a silica gel carrier, polyethylene imine as a ligand, and a spacer arm grafted or ungrafted, and the spacer arm is hexanediamine. The purified gelatin can be used in the preparation of blood plasma substitutes, tissue engineering scaffolds, implants and absorbable hemostatic materials.

The utility model relates to the technical field of embedded channels. The utility model discloses a multidirectional anchoring pre-buried channel which comprises a pre-buried groove body, a connecting assembly is connected to the inner side of the pre-buried groove body in a sliding mode, the connecting assembly further comprises a hollow sliding block, a threaded sleeve is fixedly connected to the inner side of the hollow sliding block, a threaded connecting rod is connected to the inner side of the threaded sleeve in a threaded mode, and the threaded connecting rod is fixedly connected to the inner side of the hollow sliding block. The inner side of the hollow sliding block is slidably connected with a locking part, the embedded channel is provided with the locking part, when the position of the connecting assembly needs to be adjusted, the pressing plate is pressed downwards, finally, the inserting block is pulled back into the hollow sliding block, at the moment, the hollow sliding block can slide in the embedded channel at will, the pressing plate is loosened, and the connecting assembly can be adjusted. And the inserting block is clamped with the inner wall of the pre-embedded groove body through the limiting groove. Thus, the connecting assembly is firmly fixed to the specific position in the embedded groove body, the anchoring function is achieved, the firmness of the connecting assembly during fixing is guaranteed, and the anchoring requirement is met.

This invention relates to the field of bridge construction technology, and discloses a support system and construction method for a mainline bridge cap beam. The system includes: a cap beam formwork support, which provides a supporting foundation for the mainline bridge cap beam formwork; the cap beam formwork support includes: a first cap beam formwork support and a second cap beam formwork support; multiple bracket structures connected to the existing mainline bridge piers; both ends of the bracket structures extend outward beyond the mainline bridge piers, forming cantilever ends; the first cap beam formwork support is erected on the cantilever ends of the bracket structures, with its top providing support for the mainline bridge cap beam formwork; the second cap beam formwork support is erected above the existing auxiliary road bridge deck, with its top providing support for the mainline bridge cap beam formwork; the first and second cap beam formwork supports are connected to form an integrated support system. Because the cap beam formwork support does not occupy the river channel area under the bridge, it ensures that the river's flood control and navigation functions are not affected; and it effectively shortens the construction period.

The utility model discloses a plasmid extraction column passing device which comprises a controller with a cooling unit, multi-section type telescopic rods on the controller are connected with a pressing plate, and a plurality of cooling grooves are arranged between the two multi-section type telescopic rods; a first layer plate and a second layer plate are stacked between the pressing plate and the controller, a plurality of first column passing channels with built-in filter columns are uniformly distributed on the first layer plate, a plurality of second column passing channels with built-in adsorption columns are uniformly distributed on the second layer plate, the first column passing channels and the second column passing channels are consistent in size, a first conical liquid outlet is formed in the lower end of each first column passing channel, and a second conical liquid outlet is formed in the lower end of each second column passing channel. A second conical liquid outlet is formed in the lower end of the second column passing channel; sample adding pipes in one-to-one correspondence with the first column passing channels are arranged on the pressing plate, a high-pressure air pump is arranged in the controller, an air blowing pipe is arranged on the high-pressure air pump, and air outlet branch pipes communicated with the sample adding pipes one by one are arranged at the air outlet end of the air blowing pipe. The utility model has the advantages that the plasmid extraction lysate can be easily filtered and adsorbed, the operation time is greatly shortened, and the plasmid structure is prevented from being damaged.