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151results about How to "Functionality is not affected" patented technology

Serum-free fibroblast cell culture medium and preparation method thereof

The invention relates to a serum-free fibroblast cell culture medium and a preparation method thereof, wherein the base culture medium is a DMEM culture medium or a mixture of the DMEM culture medium and a F12 culture medium, and the exogenous additives comprise hormones, a metal transporter, glutamine, a lipid concentration agent, an antioxidant, purine, saccharide and a cell growth factor. Compared with the culture medium in the prior art, the serum-free fibroblast cell culture medium of the present invention has the following advantages that: animal origin or recombinant albumin is not added so as to avoid the possibly-carried safe hidden danger and reduce the culture medium cost; the culture medium is suitable for primary culture and subculture of fibroblast cells, the good cell morphology is maintained, and the status and the function of the cultured cells are similar to the status and the function of the serum group cells; adherence factor coating is not required, the primary 24 h adherence rate is more than 60%, the passage 24 h adherence rate is more than 80%, no difference exists between the cells cultured with the culture medium of the present invention and the serum group cells; requirements of multiple passages can met, and the growth conditions of the cells after 15 passages are similar to the growth conditions of the serum group cells; and after the fibroblast cells are directly transferred into the culture of the present invention from the serum-containing culture, the cells can successfully adhere and extend without the gradual adaptation process.
Owner:西安博鸿生物技术有限公司

Antistatic environment-friendly communication equipment electrified cleaning agent

The invention relates to the field of the communication equipment electrified cleaning agent, in particular to an antistatic environment-friendly communication equipment electrified cleaning agent. The electrified cleaning agent is prepared by mixing the following components by weight: 50-65 parts of dipropylene glycol, 10-20 parts of hexamethylsiloxane or prestoxmethylsiloxane, 2-10 parts of methylsilicone oil, 10-20 parts of SMD-40 solvent oil and 0.01-1 part of an antistatic agent. The communication equipment electrified cleaning agent disclosed by the invention takes the dipropylene glycol as main raw material, other chemical reagents with strong cleaning capability are reasonably cooperated with the main raw material, so that the antistatic environment-friendly communication equipment electrified cleaning agent has good cleaning effect on pollutants on the surface of communication equipment, has the advantages of strong compatibility, no corrosion on materials, such as plastics, paints, metals and the like, high network voltage and volume resistivity, and the function of the communication equipment is free from influence. The antistatic agent used in the antistatic environmental-friendly communication equipment electrified cleaning agent is characterized in that one layer of antistatic membrane is formed on the surface of cleaned equipment to effectively enhance the anti-fouling capability of the equipment. The antistatic environment-friendly communication equipment electrified cleaning agent does not contain substance capable of damaging the ozone layer, is free from halogen and does not have bad influence on the environment.
Owner:安徽朗清科技发展有限公司

Internet of visions multi-conference test system and method

ActiveCN109462753ASolve the problem of multi-conference control performanceEasy to handleTelevision conference systemsTwo-way working systemsVirtual terminalVirtual user
The embodiment of the application provides an internet of visions multi-conference test system and a method for performing conference test based on the system. The system comprises an internet of visions server, and a web multi-conference management system; multiple virtual user terminals with virtual terminal identifiers are registered in the internet of visions server; multiple local conferencelists with list identifiers are preset in the web multi-conference management system; the local conference lists further comprise multiple virtual terminal identifiers; the internet of visions serverfurther comprises a conference room creating module, an instruction receiving module and a conference control module. In the system constructed by the embodiment of the invention, the web multi-conference management system can generate thousands of local conference lists, thousands of conference rooms can be created in the internet of visions server according to thousands of local conference lists, and the conference control can be performed on thousands of conference rooms according to a conference control instruction sent by the web multi-conference management system, and the problem that the conference test system is inexistent in the existing internet of visions is solved.
Owner:VISIONVERA INFORMATION TECH CO LTD

Block-to-stop transmission device

The invention provides a block-to-stop transmission device; the block-to-stop transmission device comprises a drive chain wheel and a transmission chain wheel; the drive chain wheel and the transmission chain wheel are concurrently circled by an enclosed chain, so as to enable the drive chain wheel and the transmission chain wheel to be driven by the chain; a control device of the drive chain wheel is arranged in a never changed position with respect to the rotation axis of the drive chain wheel; a switch of the control device always directly or indirectly pushes against the chain via an elastic device, thus the position of the switch with respect to the control device can be changed when the chain is not wrapped on the drive chain wheel, or the portion of chain on the transmission chain wheel is loosened or tightened, thereby changing the work state of the switch. The control device of the block-to-stop transmission device is matched with the chain by using the elastic device and a micro switch, so as to conveniently and effectively identify whether the output end of the transmission mechanism is blocked, and timely control the drive chain wheel to stop the power output by controlling the triggering of the switch, thereby achieving the block-to-stop function; additionally, a certain motion process exists when the chain changes from the tightened position to the loosened limit position, thus the whole mechanism has a margin for sufficient buffering, so as to effectively prevent the mechanical parts from irreversible deformation caused by emergent forward braking of the mechanical parts.
Owner:ZHANGZHOU CHANGTAI NEW QILIN MACHINE

Method for adjusting and controlling in vitro three-dimensional directed differentiation of stem cells

The invention relates to the technical field of regenerative medicine and discloses a method for adjusting and controlling in vitro three-dimensional directed differentiation of stem cells. The method includes following steps: embedding Stem cells into calcium alginate micro gel beads containing chondroitin sulfate, adding an inductive differentiation culture liquid, adjusting and controlling the in vitro directed differentiation of the stem cells by changing a mass ratio between the sodium alginate and the chondroitin sulfate during the preparation of the micro gel beads, dissolving the calcium alginate micro gel beads in a sodium citrate solution after the differentiation process being finished, and then obtaining pure differentiation cells. The method is simple in operation and low in cost, the calcium alginate micro gel beads containing chondroitin sulfate can simulate an in vivo three-dimensional extracellular matrix so that a three-dimensional directed differentiating microenvironment which approximates an in vivo microenvironment is supplied for the stem cells, thereby improving directed differentiation efficiency and a biological function of the stem cells. The method plays an important role in applications of regenerative medicine.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Brush-type online cleaning system device

The invention discloses a brush-type online cleaning system device. The brush-type online cleaning system device comprises a cooling tower, a cooling water pump, a central air-conditioning main unit condenser, a four-way reversing valve and a four-way reversing valve controller. A water inlet of the cooling tower is connected with the four-way reversing valve through a water return pipe, a water outlet of the cooling tower is connected with the cooling water pump, an outlet of the cooling water pump is connected with the four-way reversing valve through a water inlet pipe, and the other two connectors of the cooling water pump are connected with a water inlet and a water outlet of the central air-conditioning main unit condenser respectively. The brush-type online cleaning system device has the advantages that a snap joint design is adopted for brush baskets, thus a brush is convenient to replace, and the brush-type online cleaning system device is applicable to light pipes and efficient threaded pipes; the service life is long since the pipe brush is made from a special material, on the condition that the pipe brush is continuously operated for 24 hours under a standard refrigerator working condition, the service life of the pipe brush is 5 years, and there is no need to replace the pipe brush within 5 years.
Owner:威吽(深圳)科技有限公司

Targeting vector, method for constructing F4/80-DTR transgenic mouse capable of regulating, controlling and removing macrophages through diphtheria toxin, and application for targeting vector

The invention relates to the field of animal model construction, and specifically discloses a targeting vector, a method for constructing an F4/80-DTR transgenic mouse capable of regulating, controlling and removing macrophages through diphtheria toxin, and an application for the targeting vector. The targeting vector has a sequence as shown in SEQ ID NO. 8, and is named as an F4/80 5HA L-IRES-DTR-PGK-EM7-NEO-F4/80 3HA L-MCL-HSV TK vector. The invention also provides a method and application examples for cell production and cultivation of transgenic mice. According to the invention, the constructed F4/80-DTR transgenic mouse animal model for regulating, controlling and removing tissue intrinsic macrophages through the diphtheria toxin can reach the tissue intrinsic macrophage removing efficiency of 70%-90%, can well remove the tissue intrinsic macrophages, is not affected in a macrophage function when the diphtheria toxin is not used for induction, is free of side reaction when the diphtheria toxin induces to remove the macrophages, provides a brand novel principle and a method for removing the tissue intrinsic macrophages, and provides a brand novel animal model capable of inducing to remove the tissue intrinsic macrophages.
Owner:THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE

Micro-vibration suppression platform based on intelligent piezoelectric array and control method thereof

The invention discloses a micro-vibration suppression platform based on an intelligent piezoelectric array. The micro-vibration suppression platform comprises an upper platform, a lower platform, main dampers and secondary dampers, wherein the main dampers and the secondary dampers are uniformly distributed between the upper platform and the lower platform; the main dampers and the secondary dampers are inclined; extension lines of the axes of the main dampers are intersected at a point above the upper platform; each damper comprises an outer sleeve, main shaft rods located on the central axis of the damper and a piezoelectric stack array arranged in the middle of the outer sleeve; each piezoelectric stack array at least comprises two piezoelectric stacks; the piezoelectric stacks are symmetrically distributed relative to the main shaft rods; and the centers of the arrays are hollow. Through the micro-vibration suppression platform, control and suppression of the micro-vibration signals in six degrees of freedom are realized, so that comprehensive suppression of the micro-vibration signals from all directions and motion dimensions is realized; the reliability of the vibration suppression platform is greatly improved; and the micro-vibration signals with low frequency and high anisotropy and randomness can be accurately suppressed in all directions and dimensions.
Owner:YANCHENG INST OF TECH

Anti-freezing method suitable for polar sailing ship

The invention discloses an anti-freezing method suitable for a polar sailing ship. The anti-freezing method is characterized in that the anti-freezing manner for pipelines is a mixed composition manner formed by combining adjoining-pipe anti-freezing structure heating, heat preservation materials, a residue discharging method and a gas drying method; the anti-freezing manner for mechanical compartments and liquid tanks is a mixed composition manner formed by combining steam heating, electric heating, electric heaters and the heat preservation materials; the anti-freezing manner for equipment is a mixed composition manner formed by combining heating and enclosed protection; the anti-freezing manner for channels is formed by the electric heating and the enclosed protection or a mixed combination of the electric heating and the enclosed protection. The method disclosed by the invention has the advantages that on the consideration of pipeline systems, the mechanical compartments, the liquid tanks, the equipment and the channels and the like, various important systems and equipment of the polar sailing ship are effectively guaranteed to normally operate in severe environments, such as low air temperature, low water temperature and the like, and functions of various compartments and channels are not influenced, therefore the polar sailing ship is guaranteed to safely sail in cold sea areas.
Owner:NANTONG COSCO KHI SHIP ENG

Method for promoting directional differentiation and proliferation of mesenchymal stem cells towards neural precursor cells

The invention provides a method for promoting directional differentiation and proliferation of mesenchymal stem cells towards neural precursor cells. The method comprises the following steps: firstly, embedding the mesenchymal stem cells in calcium alginate microbeads which are prepared with the presence of a calcium chloride solution and contains gelatin microspheres and chondroitin sulfate; then, adding a neural induction differentiation medium, and cultivating in a rotary reactor, so as to promote the in vitro directional differentiation and proliferation of the mesenchymal stem cells towards the neural precursor cells; and after differentiation, dissolving the calcium alginate microbeads by virtue of a sodium citrate solution, so that differentiated cells are obtained. The method disclosed by the invention is simple to operate and low in cost; by virtue of the gelatin microspheres, a necessary growth space is provided for internal cells of a stent; by virtue of the chondroitin, the biochemical functions of a neural tissue extracellular matrix can be simulated; by virtue of the calcium alginate microbeads, three-dimensional support, which is similar to the extracellular matrix, is provided; and by virtue of the rotary reactor, a necessary micro-gravity environment is provided, so that mass transfer is further enhanced and proliferation of the cells is achieved.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Annular header pipe type bilge system for ocean nuclear power platform

The invention discloses an annular header pipe type bilge system for an ocean nuclear power platform, relates to the technical field of bilge systems, and aims to solve the problem of influence on running of the entire system since the header pipe type bilge system tends to leak when a certain segment of pipeline fails in the prior art. In the bilge system, each bilge water suction port is connected with an annular header pipe through a fluid-controlled cut-off check valve; the annular header pipe is partitioned into a plurality of segments through isolating valves; bilge pumps are connected to the header pipe through cut-off valves. The isolating valves are in open states normally; when water is accumulated in a certain cabin and needs draining, the fluid-controlled cut-off check valve and the cut-off valves corresponding to the cabin are opened remotely, and the bilge pump is started, so that bilge water can be drained. The annular header pipe and the isolating valves are provided, so that the redundancy degree of the system functions is increased: when a certain segment of pipeline fails, the failed segment of pipeline can be isolated through the isolating valves, and the header pipe consisting of the rest of the segments of the pipelines can still run normally, the system functions are not influenced, normal running of the system is ensured, and the system reliability is improved.
Owner:NO 719 RES INST CHINA SHIPBUILDING IND +1

Antibacterial and anti-mite organic cotton composite fiber fabric and processing process thereof

The invention discloses antibacterial and anti-mite organic cotton composite fiber fabric and a processing process thereof. The antibacterial and anti-mite organic cotton composite fiber fabric is formed by mutually interweaving warp yarns and weft yarns; the warp yarns are made of organic cotton fiber and the weft yarns are made of polyester fiber; the proportion of the organic cotton fiber is 24percent and the proportion of the polyester fiber is 76 percent; antibacterial and anti-mite aids are arranged in the organic cotton fiber and the polyester fiber; the antibacterial and anti-mite aids are fixed in the organic cotton fiber and the polyester fiber and are protected by the organic cotton fiber and the polyester fiber; the antibacterial and anti-mite aid comprises quaternary ammoniumsalt and organic silicon; 10 percent of bamboo fiber is added in the organic cotton fiber; the organic cotton fiber and the bamboo fiber are blended; and the antibacterial and anti-mite cloth can damage the cell walls of bacteria and prevent the metabolic process of microorganisms, so that the microorganisms cannot grow and reproduce. Reproduction of mites is inhibited and peculiar smell caused by the microorganisms is prevented. The antibacterial, mildew-proof and anti-mite agent can effectively resist bacteria and prevent mites and has good anti-mite effect.
Owner:SIMMONS BEDDING&FURNITURE (SUZHOU) LTD
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