Preparation of quality control reference strain for myeloid leukemia fusion gene detection
A fusion gene and leukemia technology, applied in the medical field, can solve the problems of affecting the detection method, unable to repeat, unable to transform bone marrow cells, etc.
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[0013] figure 1 It is the clone diagram of the hybrid cell prepared by the present invention.
[0014] in figure 1 In, granulocytes of various stages differentiated by myelogenous leukemia patients induced by granulocyte colony stimulating factor, fused with murine myeloma cells under the action of PEG, were screened by HAT for 1 to 2 weeks, and under an inverted microscope Take a picture (40X) to obtain a hybrid cell clone map.
[0015] Combine below figure 1 , A detailed description of the embodiments of the present invention.
[0016] 1. Cells to be fused
[0017] (1) Bone marrow cells to be fused: the bone marrow cells of patients with granulocytic leukemia remaining after the diagnosis and bone marrow cytology are collected, containing BCR-ABL, PML-RARAα, AML1 / ETO and / or PML-RARa fusion genes.
[0018] (2) Myeloma cells: mouse SP2 / 0 tumor cells, purchased from Sigma.
[0019] 2. Experimental reagents
[0020] (1) Granulocyte colony stimulating factor
[0021] (2) DMEM medium, HAT ...
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