Preparation method of specific extracellular matrix ECM of periodontium and application thereof

A periodontal tissue and periodontal ligament cell technology, which is used in the preparation of extracellular matrix, can solve problems such as the influence of periodontal tissue and periodontal ligament stem cells, and achieve the purpose of promoting in vitro proliferation and directional differentiation. Effect

Active Publication Date: 2014-08-06
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

So far, there are no reports on the preparation of periodontal tissue-specific ECM and its effect on periodontal ligament stem cells

Method used

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  • Preparation method of specific extracellular matrix ECM of periodontium and application thereof
  • Preparation method of specific extracellular matrix ECM of periodontium and application thereof
  • Preparation method of specific extracellular matrix ECM of periodontium and application thereof

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Embodiment Construction

[0033] The present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings, which are explanations rather than limitations of the present invention.

[0034] The invention provides a simple and efficient preparation method of periodontal tissue-specific ECM (hPDLCs-ECM), and the prepared ECM can be used to massively expand human periodontal ligament stem cells hPDLSCs in vitro and promote their directional differentiation.

[0035] Taking the preparation method of human periodontal ligament tissue-specific ECM as an example, the specific steps include primary culture of human periodontal ligament cells, subculture, preparation and detection of ECM, and the use of the ECM to expand human periodontal ligament stem cells hPDLSCs in vitro .

[0036] 1) Isolation and culture of human periodontal ligament cells hPDLCs, the specific method is:

[0037] Collect clinically fresh permanent bicuspids and third molars aged 12...

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Abstract

The invention relates to a preparation method of a specific extracellular matrix ECM of periodontium. The preparation method comprises the following steps: 1) separating and culturing a human periodontal ligament cell; and 2) preparing the specific extracellular matrix ECM of periodontium; applying the specific extracellular matrix ECM of periodontium and applying the specific extracellular matrix ECM of periodontium as a human periodontal ligament stem cell in in-vitro amplification; and promoting directional differentiation of the ECM. The preparation method is simple and can remarkably promote in-vitro amplification and directional differentiation of the periodontal ligament stem cell.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of extracellular matrix derived from periodontal tissue and its application in aspects such as periodontal ligament stem cell culture and expansion. Background technique [0002] Periodontitis is one of the oldest and most common diseases of mankind. It is a chronic infectious disease of periodontal support tissue caused by microorganisms in dental plaque, which can lead to inflammation of periodontal support tissue, periodontal pocket formation, progressive loss of attachment and alveolar bone resorption, and eventually cause tooth loosening and was removed. According to statistics, the prevalence and severity of periodontitis increase with age, and the prevalence increases significantly after the age of 35, reaching a peak at the age of 50 to 60. At present, the prevalence rate of periodontitis in my country has exceeded 80%, and it is the first cause of tooth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/074
Inventor 武俊杰孙薇丁寅陈晓东王阿娴唐丽
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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