Application of caffeic acid derivatives to promotion of in-vitro proliferation of umbilical cord mesenchymal stem cells and preparation of proliferation promoting culture medium

A technology of stem cells and in vitro proliferation, applied in the field of stem cells, can solve problems such as tissue cell damage and differences in therapeutic effects

Inactive Publication Date: 2020-09-29
南京温博生物科技有限公司
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Problems solved by technology

In clinical practice, the end result of the continuous development of most diseases is tissue cell damage, which can be treated with umbilical cord mesenchymal stem cell transplantation and may produce certain curative effect, but because the degree of cell damage is different in different stages of disease, the curative effect may there will be a big difference

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  • Application of caffeic acid derivatives to promotion of in-vitro proliferation of umbilical cord mesenchymal stem cells and preparation of proliferation promoting culture medium
  • Application of caffeic acid derivatives to promotion of in-vitro proliferation of umbilical cord mesenchymal stem cells and preparation of proliferation promoting culture medium
  • Application of caffeic acid derivatives to promotion of in-vitro proliferation of umbilical cord mesenchymal stem cells and preparation of proliferation promoting culture medium

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Embodiment Construction

[0015] The substantive content of the present invention will be described below in conjunction with specific embodiments, but the protection scope of the present invention cannot be limited thereto.

[0016] 1. Experimental materials

[0017] The cell incubator is a product of Thermo Fisher Scientific.

[0018] The flow cytometer is a product of BD Biosciences, USA.

[0019] Fetal bovine serum was the product of Gibco in the United States, α-MEM medium was the product of Hyclone, and trypsin was purchased from Beyond.

[0020] The chemical structure of caffeic acid-4-2-hydroxyethylmorpholine ester is as follows figure 1 As shown, the purity is 99%.

[0021] Human umbilical cord mesenchymal stem cell osteogenic differentiation medium was purchased from Saiye Biotechnology, Cat. No. HUXUC-90021.

[0022] Human umbilical cord mesenchymal stem cell adipogenic differentiation medium was purchased from Saiye Biotechnology, Cat. No. HUXUC-90031.

[0023] 2. Experimental method ...

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Abstract

The invention discloses application of caffeic acid derivatives to promotion of in-vitro proliferation of umbilical cord mesenchymal stem cells and preparation of a proliferation promoting culture medium. By experiments in the invention, the following result can be found that caffeic acid-4-2-hydroxyethyl morpholine ester can obviously promote in-vitro proliferation of hUCMSCs (human umbilical cord mesenchymal stem cells), has a dose-dependent effect and a time-dependent effect, and the hUCMSCs proliferated by the caffeic acid-4-2-hydroxyethyl morpholine ester are still kept having very high multi-directional differentiation capacity and cna be used as seed cells. Therefore, the caffeic acid-4-2-hydroxyethyl morpholine ester can be used for promoting in-vitro proliferation of the hUCMSCs and used for preparing a culture medium for promoting in-vitro proliferation of the hUCMSCs.

Description

technical field [0001] The invention belongs to the field of stem cells, and relates to the in vitro proliferation of umbilical cord mesenchymal stem cells, in particular to the use of a caffeic acid derivative to promote the in vitro proliferation of umbilical cord mesenchymal stem cells and to prepare a proliferation-promoting medium. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cells with self-renewal ability and multi-lineage differentiation potential, which widely exist in tissues such as bone marrow, umbilical cord, placenta and adipose tissue. In addition to supporting hematopoiesis and preventing graft-versus-host disease, MSCs can also repair tissue damage such as bone and cartilage, tendon, myocardium, and skin, and are considered ideal seed cells for tissue engineering and cell therapy. Among them, umbilical cord mesenchymal stem cells (hUCMSCs) have high differentiation potential and can differentiate in multiple dire...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2500/30
Inventor 姜东花谷超
Owner 南京温博生物科技有限公司
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