Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

BFGF-loaded guided tissue regeneration membrane with core-shell structure and preparation method of bFGF-loaded guided tissue regeneration membrane

A technology that guides tissue regeneration and core-shell structure. It is applied in the field of biomedicine and can solve the problems of blended fibers that do not have drug control release, "burst release, and protein molecular structure destruction, etc., and achieve good cell compatibility and mild film-forming conditions. , the effect of simple preparation method

Inactive Publication Date: 2020-04-10
NINGXIA MEDICAL UNIV
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional electrospinning technology physically blends drugs and organic polymers to prepare composite nanofibers as a drug delivery system. However, when the drug to be transported is an active macromolecule, this blending method will not only affect the molecular structure of the protein. damage, and the blended fiber does not have the ability to control drug release, and it is prone to "burst release" phenomenon in the early stage of drug release

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • BFGF-loaded guided tissue regeneration membrane with core-shell structure and preparation method of bFGF-loaded guided tissue regeneration membrane
  • BFGF-loaded guided tissue regeneration membrane with core-shell structure and preparation method of bFGF-loaded guided tissue regeneration membrane
  • BFGF-loaded guided tissue regeneration membrane with core-shell structure and preparation method of bFGF-loaded guided tissue regeneration membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] 1) Configuration of aqueous solution: Dissolve basic fibroblast growth factor (bFGF) freeze-dried powder in phosphate buffered saline (PBS) containing bovine serum albumin (BSA) to form basic fibroblast growth factor / Bovine serum albumin solution, wherein the content of basic fibroblast growth factor (bFGF) is 0.5g / L, and the content of bovine serum albumin is 0.5% (w / v);

[0079] Dissolve dextran (DEX) in phosphate buffered saline (PBS), stir well to form a dextran solution with a concentration of 0.13g / mL;

[0080] Mix the basic fibroblast growth factor / bovine serum albumin solution with the dextran solution at a ratio of 1:15 to obtain a mixed solution of dextran / basic fibroblast growth factor / bovine serum albumin, which is used as the aqueous phase solution for subsequent use, and the final water The concentration of bFGF in the phase solution is 0.03g / L;

[0081] 2) Configuration of oil phase solution: Dissolve polylactic acid-glycolic acid copolymer (PLGA) in a ...

Embodiment 2

[0084] 1) Configuration of aqueous solution: Dissolve basic fibroblast growth factor (bFGF) freeze-dried powder in phosphate buffered saline (PBS) containing bovine serum albumin (BSA) to form basic fibroblast growth factor / Bovine serum albumin solution, wherein the content of basic fibroblast growth factor (bFGF) is 2.0g / L, and the content of bovine serum albumin is 0.5% (w / v);

[0085] Dissolve dextran (DEX) in phosphate buffered saline (PBS), stir well to form a dextran solution with a concentration of 0.12g / mL;

[0086] Mix the basic fibroblast growth factor / bovine serum albumin solution with the dextran solution at a ratio of 1:13 to obtain a mixed solution of dextran / basic fibroblast growth factor / bovine serum albumin, which is used as the aqueous phase solution for subsequent use, and the final water The concentration of bFGF in the phase solution is 0.15g / L;

[0087] 2) Configuration of oil phase solution: Dissolve polylactic acid-glycolic acid copolymer (PLGA) in a ...

Embodiment 3

[0090] 1) Configuration of aqueous solution: Dissolve basic fibroblast growth factor (bFGF) freeze-dried powder in phosphate buffered saline (PBS) containing bovine serum albumin (BSA) to form basic fibroblast growth factor / Bovine serum albumin solution, wherein the content of basic fibroblast growth factor (bFGF) is 4.0g / L, and the content of bovine serum albumin is 0.1% (w / v);

[0091] Dissolve dextran (DEX) in phosphate buffered saline (PBS), stir well to form a dextran solution with a concentration of 0.12g / mL;

[0092] Mix the basic fibroblast growth factor / bovine serum albumin solution with the dextran solution at a ratio of 1:14 to obtain a mixed solution of dextran / basic fibroblast growth factor / bovine serum albumin, which is used as the aqueous phase solution for subsequent use, and the final water The concentration of bFGF in the phase solution is 0.27g / L;

[0093] 2) Configuration of oil phase solution: Dissolve polylactic acid-glycolic acid copolymer (PLGA) in a ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a bFGF-loaded guided tissue regeneration membrane with a core-shell structure and a preparation method of the bFGF-loaded guided tissue regeneration membrane. The guided tissue regeneration membrane is constructed from basic fibroblast growth factors, synthetic polymer compound polylactic acid-glycolic acid copolymers and natural component wool keratin. The composite membrane provided by the invention can prevent fibroblasts from surrounding tissues from migrating to a defect area, so that periodontal ligament cells preferentially migrate, attach, proliferate and differentiate on the tooth root surface; the sustained release of the growth factor can actively regulate and control the cell function and accelerate the induction of the growth, proliferation and differentiation of cells, so as to obtain more periodontal tissue regeneration. The guided tissue regeneration membrane is simple in preparation method and mild in membrane forming condition, has good cytocompatibility and performance of actively inducing regeneration of periodontal tissues, meets the requirements of in-vivo application, and has good application prospects as a biological membrane with atissue regeneration inducing effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a bFGF-loaded guided tissue regeneration membrane with a core-shell structure and a preparation method thereof. Background technique [0002] Periodontal disease is a chronic, nonspecific, infectious disease that can cause damage to tooth supporting tissues (gingiva, periodontal ligament, alveolar bone, and cementum), eventually leading to tooth loosening and loss. It is one of the most common oral diseases in humans and the leading cause of tooth loss in adults. Periodontal disease not only affects oral health, but also has a certain impact on overall health. [0003] The goal of periodontal disease treatment is to prevent the progression of the disease and to regenerate the lost periodontal tissue. Among them, the regeneration of periodontal tissue is the key to successful treatment. Traditional treatment methods such as scaling, root planing, and bone grafting can only ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/22A61L27/54
CPCA61L27/227A61L27/18A61L27/54A61L2300/414C08L89/00C08L67/04
Inventor 张华林王凯戎
Owner NINGXIA MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com