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Nerve regeneration gel as well as preparation method and application thereof

A nerve regeneration and gel technology, applied in biochemical equipment and methods, nervous system diseases, pharmaceutical formulations, etc., can solve the problems of difficult to form gel and easy to lose, so as to improve the antioxidant capacity, reduce the level, and facilitate the operation The effect used in

Active Publication Date: 2018-05-01
镇江市中西医结合医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fibronectin and laminin are difficult to form a gel, and they are easily lost when injected into the injury site

Method used

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  • Nerve regeneration gel as well as preparation method and application thereof
  • Nerve regeneration gel as well as preparation method and application thereof
  • Nerve regeneration gel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of nerve regeneration gel

[0027] Phosphate buffered saline (PBS) with a pH of 7.4 was prepared with deionized water and sterilized at high temperature for later use. Prepare 250U / ml thrombin solution (component B) with PBS; prepare 25mg / ml sterile fibrinogen solution with PBS, then add fibronectin and laminin in turn to the fibrinogen solution (final concentration is 0.125mg / ml), proanthocyanidins (concentration: 12.5mg / ml), sonic hedgehog factor, epidermal growth factor, neurotrophin-3 and vascular endothelial growth factor (concentration: 125ng / ml), this is component A. Mix 4 times the volume of component A with 1 part of component B to obtain a transparent gel, which is the nerve regeneration gel of the present invention.

[0028] 2. In vitro cell test

[0029] 1. Nerve regeneration gel was prepared as described in Example 1

[0030] 2. Experimental grouping

[0031] According to the culture conditions, it is divided into four groups:

[0032] ...

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PUM

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Abstract

The invention discloses a nerve regeneration gel as well as a preparation method and application thereof. The matrix of the nerve regeneration gel is formed by fibrinogen, thrombin, laminin and fibronectin; the nerve regeneration gel contains procyanidine, sonic hedgehog, an epidermal growth factor, neurenergen-3 and a vascular endothelial growth factor. In-vitro test and animal test show that thegel is capable of accelerating neuron regeneration and neurite extension and repairing the injured nerve tissue. The matrix of the gel comprises fibrin, laminin and fibronectin and is capable of reducing formation of cavities and accelerating adhesion and proliferation of endogenous neural stem cells; the procyanidine in the gel is capable of resisting oxidation and inhibiting inflammation; the sonic hedgehog, the epidermal growth factor and the neurenergen-3 are capable of accelerating proliferation of the stem cells and differentiation to neurons of the stem cells; the vascular endothelialgrowth factor is capable of accelerating generation of blood vessels. The gel can be formed in situ on injured parts and can be used for treating nervous system injury.

Description

technical field [0001] The invention relates to a regeneration gel, in particular to a nerve regeneration gel and its preparation method and application, and belongs to the field of biological materials. Background technique [0002] Injuries to the central nervous system, especially spinal cord injuries, are often caused by trauma. A large number of cells will die at the moment of the blow, and at the same time, the myelin sheath will begin to disintegrate, and spinal cord softening will appear at the injured site. A large number of cells and myelin sheath fragments are attacked by high oxygen partial pressure under hemorrhage, and lipid peroxidation occurs. In the acute phase, a large number of free radicals are generated, causing severe oxidative damage to the injured site. Therefore, some studies have proposed to supplement antioxidants to patients with spinal cord injury. On the other hand, there is a blood-spinal cord barrier in the spinal cord, which is destroyed aft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61K9/06A61P25/00A61P25/28A61P29/00A61P39/06A61K38/39A61K31/352A61K38/18A61K38/36
CPCA61K9/0024A61K9/06A61K31/352A61K38/18A61K38/1808A61K38/185A61K38/1866A61K38/363A61K38/39A61K38/4833C12Y304/21005A61K2300/00
Inventor 胡萍萍陈谦宗斌张志坚
Owner 镇江市中西医结合医院
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