Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for culturing adipocytes

A technology for adipocytes and mature adipocytes, applied in animal cells, tissue culture, vertebrate cells, etc.

Inactive Publication Date: 2013-03-20
UNIV PIERRE & MARIE CURIE +1
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Therefore, these systems do not appear to specifically and directly represent the metabolism of mature adipocytes isolated from adipose tissue.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for culturing adipocytes
  • Method for culturing adipocytes
  • Method for culturing adipocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] As intended herein, the expression "mature adipocyte" means a differentiated adipocyte. Mature adipocytes can be readily identified by those skilled in the art. In particular, mature adipocytes according to the invention generally have a single lipid vacuole, lipolytic activity, in particular induced by beta-adrenergic agents such as epinephrine and norepinephrine, and are also insulin sensitive, Specifically defined as the insulin-induced transport of glucose and fatty acids from the extracellular medium to the intracellular medium, which is associated inter alia with the insulin-dependent translocation of the GLUT-4 and CD36 transporters, respectively, within the plasma membrane of mature adipocytes . Mature adipocytes according to the invention differ in particular from preadipocytes, especially in the expression of the preadipocyte-specific markers DelK1 / Pref1 known to those skilled in the art (which can be measured by real-time PCR), in which according to the inve...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for culturing adipocytes, in which a homogeneous population of mature adipocytes isolated from adipose tissue is cultured on a three-dimensional culture substrate.

Description

technical field [0001] The present invention relates to methods for culturing adipocytes, to adipocyte cultures, and to their use for screening compounds that regulate adipocyte metabolism. Background technique [0002] The prevalence of obesity, associated with white adipose tissue hypertrophy, continues to increase in the general public. Thus, the proportion of overweight or obese individuals has increased from 36.7% to 41.6% in France between 1997 and 2003; moreover, the prevalence of obesity (defined as greater than 30 kg / m 2 The body mass index (BMI (IMC in French original))) is 14.5%. Furthermore, obesity is a risk factor for many pathological diseases, including especially cardiovascular events, which makes its therapeutic treatment even more urgent. [0003] However, improving the diet and increasing physical activity of obese individuals remains the main way of combating the epidemic, with other approaches, especially surgical and drug-based, being explored. [0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077G01N33/50
CPCG01N33/5044C12N5/0653C12N2501/39C12N2533/50G01N33/50
Inventor D·拉卡萨V·佩莱格里内利M·乔菲帕提K·克莱芒
Owner UNIV PIERRE & MARIE CURIE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com