Method for culturing adipocytes

A technology for adipocytes and mature adipocytes, applied in animal cells, tissue culture, vertebrate cells, etc.

Inactive Publication Date: 2013-03-20
UNIV PIERRE & MARIE CURIE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Therefore, these systems do not appear to specifically and directly represent the metabolism of mature adipocytes isolated from adipose tissue.

Method used

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  • Method for culturing adipocytes
  • Method for culturing adipocytes
  • Method for culturing adipocytes

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Embodiment Construction

[0019] As intended herein, the expression "mature adipocyte" means a differentiated adipocyte. Mature adipocytes can be readily identified by those skilled in the art. In particular, mature adipocytes according to the invention generally have a single lipid vacuole, lipolytic activity, in particular induced by beta-adrenergic agents such as epinephrine and norepinephrine, and are also insulin sensitive, Specifically defined as the insulin-induced transport of glucose and fatty acids from the extracellular medium to the intracellular medium, which is associated inter alia with the insulin-dependent translocation of the GLUT-4 and CD36 transporters, respectively, within the plasma membrane of mature adipocytes . Mature adipocytes according to the invention differ in particular from preadipocytes, especially in the expression of the preadipocyte-specific markers DelK1 / Pref1 known to those skilled in the art (which can be measured by real-time PCR), in which according to the inve...

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Abstract

The present invention relates to a method for culturing adipocytes, in which a homogeneous population of mature adipocytes isolated from adipose tissue is cultured on a three-dimensional culture substrate.

Description

technical field [0001] The present invention relates to methods for culturing adipocytes, to adipocyte cultures, and to their use for screening compounds that regulate adipocyte metabolism. Background technique [0002] The prevalence of obesity, associated with white adipose tissue hypertrophy, continues to increase in the general public. Thus, the proportion of overweight or obese individuals has increased from 36.7% to 41.6% in France between 1997 and 2003; moreover, the prevalence of obesity (defined as greater than 30 kg / m 2 The body mass index (BMI (IMC in French original))) is 14.5%. Furthermore, obesity is a risk factor for many pathological diseases, including especially cardiovascular events, which makes its therapeutic treatment even more urgent. [0003] However, improving the diet and increasing physical activity of obese individuals remains the main way of combating the epidemic, with other approaches, especially surgical and drug-based, being explored. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077G01N33/50
CPCG01N33/5044C12N5/0653C12N2501/39C12N2533/50G01N33/50
Inventor D·拉卡萨V·佩莱格里内利M·乔菲帕提K·克莱芒
Owner UNIV PIERRE & MARIE CURIE
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