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Culture dish for mature adipocyte ceiling culture

A technology for mature fat cells and ceiling culture, which is applied in tissue cell/virus culture devices, special-purpose bioreactors/fermenters, biochemical instruments, etc. It can solve the problem that culture bottles are difficult to meet operational requirements, and culture dishes have not yet been seen. problems, to achieve the effect of ensuring oxygen supply and CO2 discharge, reasonable structural design, and promoting gas exchange

Inactive Publication Date: 2016-08-10
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the existing utensils for ceiling culture are mainly commercialized culture bottles, and no culture dishes for ceiling culture have been seen yet.
In some experiments, the culture bottle is difficult to meet the operational requirements of the experiment, and the culture dish has irreplaceable advantages

Method used

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  • Culture dish for mature adipocyte ceiling culture

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Effect test

Embodiment 1

[0023] see figure 1 , a petri dish used for the ceiling culture of mature fat cells, comprising a large petri dish 1 and one or several small petri dishes 2 placed upside down in the large petri dish 1, and one or several U-shaped tubes 3, U-shaped One end of the tube 3 extends into the inverted small petri dish 2, and the other end stretches out from the surface of the culture solution in the large petri dish. The lower edge of the small petri dish 2 is placed on the U-shaped tube 3, the diameter of the U-shaped tube 3 is 1-3 mm, and one end of the U-shaped tube 3 protruding from the surface of the culture solution is connected with the sample adding device. The top of the large petri dish 1 is also provided with a petri dish top cover 5 .

[0024] A clamping portion 4 is also provided on the side wall of the small petri dish 2 . Alternatively, an opening with a width of 2 to 4 mm is provided at the mouth of the small petri dish 2 .

[0025] Preferably, the number of U-sha...

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Abstract

The invention discloses a culture dish for mature adipocyte ceiling culture, and belongs to the technical field of biological medicine experimental devices. One or more inverted small culture dishes are arranged in an upright large culture dish; a U-shaped tube is arranged in the large culture dish; one end of each U-shaped tube is exposed outside the culture solution of the large culture dish; one end of each U-shaped tube is positioned inside each inverted small culture dish. No air is retained at the bottoms inside the inverted small culture dishes; meanwhile, exchange of liquids inside the inverted small culture dishes with external air can be promoted; meanwhile, the U-shaped tubes can be connected with a sampling device, and then mature adipocyte can be injected into the inverted small culture dishes for culture. The culture dish is reasonable in structural design, simple to operate, and capable of effectively culturing mature adipocyte.

Description

technical field [0001] The invention belongs to the technical field of biomedical experimental devices, and relates to a culture dish, in particular to a culture dish used for ceiling culture of mature fat cells. Background technique [0002] Adipocytes play an important role in the regulation of body metabolism. They not only serve as storage units for energy substances, but also actively regulate body metabolism under different nutritional states by releasing active factors. Abnormal function of adipocytes, such as obesity caused by excessive accumulation of lipids, seriously affects human health. It is an important content in the field of endocrinology and metabolism to cultivate adipocytes in vitro and further study their functions. Because mature adipocytes contain triglycerides, their density is lower than that of aqueous solution, and they are suspended on the surface of the culture medium during culture. Therefore, the ceiling method is often used for the culture of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/22
CPCC12M23/10
Inventor 赵玉峰苏兴利徐曦闫爱丽陈镝谢荣刘英光
Owner XIAN MEDICAL UNIV
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