Group B streptococcus fluorescent quantitative PCR detection kit

Abstract

The invention belongs to the technical field of bacteria detection, and more specifically discloses a group B streptococcus fluorescent quantitative PCR detection kit. The group B streptococcus fluorescent quantitative PCR detection kit comprises a group of group B streptococcus fluorescent quantitative PCR detection primers and probes; the primers and probes comprise GBS amplification primer pairGBS-F and GBS-R, GBS detection probe GBS-P, internal reference primer pair beta-actin-F and beta-actin-R, and internal reference probe beta-actin-P; the nucleotide sequences of GBS-F, GBS-R, GBS-P, beta-actin-F, beta-actin-R, and beta-actin-P are represented by SEQ ID NO:1-6 respectively. The group B streptococcus fluorescent quantitative PCR detection kit can be used for detecting GBS in samplewith complex composition, and detecting single copy templates, is wider in linearity range, is capable of avoiding false positive results caused by amplification products pollution, and is high in sensitivity and specificity.

Description

technical field [0001] The invention relates to the technical field of bacterial detection, in particular to a fluorescent quantitative PCR detection kit for group B streptococci. Background technique [0002] Group B streptococcus (group B streptococcus, GBS) is an aerobic Gram-positive streptococcus, which normally resides in the vagina and rectum, and is an opportunistic pathogen. Since the polysaccharides in the cell wall belong to group B in the classification of antigen structure, group B streptococcus is generally used to replace the original name of Streptococcus agalactiae. In the 1970s, GBS has been proved to be one of the main pathogenic bacteria of perinatal mother-infant infection, and it occupies a non-negligible position in perinatal medicine. At the same time, it is also the most common cause of infant sepsis and meningitis. According to statistics, the carrier rate of pregnant women is about 5% to 30%, of which 40% to 70% will be passed to the newborn durin...

AI Technical Summary

Problems solved by technology

(1) Bacterial culture method: Due to the need for bacterial culture, it takes at least 2 days to identify positive, and at least 3 days to identify negative, and the immunological sensitivity of bacterial culture will be affected by the amount of bacteria. If the amount of bacteria is insufficient, it will be difficult GBS was detected positive, so the phenomenon of missed diagnosis in clinical testing occurs from time to time

(2) Immunological method: Although this method has high specificity, its sensitivity is not enough, the detection rate is low, and it cannot meet the requirements of rapid and early diagnosis

However, the existing fluorescent quantitative PCR technology still has shortcomings such as false positives, low accuracy and stability, and low sensitivity, which limits its clinical application.

In addition, when the sample to be tested contains complex components (such as whole blood, mucin, urine, feces, human genomic DNA, clotrimazole suppository, miconazole suppository, nystatin ointment, imodium or compound carrageenan Ester suppositories, etc.), the accuracy and sensitivity of existing kits for GBS detection are often not ideal

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