Ru(bpy)3-doped Ag@SiO2 fluorescent nano particles and preparation method thereof

A ruthenium terpyridine, fluorescent nanotechnology, applied in chemical instruments and methods, luminescent materials, color/spectral property measurement, etc., to achieve the effects of good photostability, good water solubility, and good biocompatibility

Inactive Publication Date: 2010-10-06
SHANGHAI UNIV
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Problems solved by technology

[0002] Fluorescence detection using organic fluorescent dyes as fluorescent probes is a commonly used analysis and testing technology in the fields of biological science, biotechnology, and diagnostics. There are still some limitations due to such reasons, so people have been working on exploring various physical and chemical ways to enhance the fluorescence intensity of fluorescent probes for many years.

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  • Ru(bpy)3-doped Ag@SiO2 fluorescent nano particles and preparation method thereof
  • Ru(bpy)3-doped Ag@SiO2 fluorescent nano particles and preparation method thereof
  • Ru(bpy)3-doped Ag@SiO2 fluorescent nano particles and preparation method thereof

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Embodiment Construction

[0021] 1. Reagents and instruments

[0022] Ruthenium terpyridine (Ru(bpy) 3 ), TritonX-100, NaBH 4 , 3-aminopropyltrimethoxysilane (APTMS) was purchased from SIGMA company; AgNO 3 , hydrazine hydrate, and sodium citric acid were purchased from China Pharmaceutical Group Shanghai Chemical Reagent Co., Ltd.; n-hexanol, tetraethyl orthosilicate (TEOS), and ammonia water were purchased from Shanghai Haoshen Chemical Reagent Co., Ltd.; the above reagents were analytical reagents. The experimental water was ultrapure water (18MΩ).

[0023] CR21GⅡ high-speed refrigerated centrifuge (Hitachi, Japan); F-7000 fluorescence spectrophotometer (Hitachi, Japan); U-3010 ultraviolet-visible spectrophotometer (Hitachi, Japan); JEOL 200CX transmission electron microscope (Japan Electronics Co., Ltd. company)

[0024] 2. Experimental method

[0025] 1.Ru(bpy) 3 Doped Ag@SiO 2 Preparation of core-shell fluorescent nanoparticles

[0026] Add 1.77mL cyclohexane, 7.5mL n-hexanol, and 1.8mL T...

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Abstract

The invention relates to Ru(bpy)3-doped Ag@SiO2 fluorescent nano particles and a preparation method thereof. The fluorescent nano particles take Ru(bpy)3-doped silver as an inner core, silica with a net structure is coated on the surface of Ru(bpy)3, and an active amino group is positioned on the surface of the silica, wherein the mass ratio of the Ru(bpy)3 to silver is 1:1-1:10; and the mass ratio of the inner core to the silica is 1:5-1:12, and per milligram of nano particles contains 70-80nmol of amino. In the invention, a simple reverse microemulsion method is adopted to prepare the novelRu(bpy)3-doped core-shell type Ag@SiO2 nano particles. The nano particles have strong fluorescent signal, good light stability, good water-solubility and good biocompatibility; and as the active amino groups are positioned on the surface of the nano particles, the nano particles can directly react with biomolecules without surface modification.

Description

technical field [0001] The present invention relates to a kind of Ag@SiO 2 Fluorescent nanoparticles and methods for their preparation. Especially a ruthenium terpyridine Ru(bpy) 3 Doped Ag@SiO 2 Fluorescent nanoparticles and methods for their preparation. Background technique [0002] Fluorescence detection using organic fluorescent dyes as fluorescent probes is a commonly used analysis and testing technology in the fields of biological science, biotechnology, and diagnostics. There are still some limitations due to such reasons, so people have been working on exploring various physical and chemical ways to enhance the fluorescence intensity of fluorescent probes for many years. With the development of nanotechnology, it is found that core-shell silicon fluorescent nanoparticles are prepared by wrapping many fluorescent dye molecules in the same silicon sphere. Since a large number of fluorescent molecules can be wrapped in one silicon shell at the same time, the fluore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/02G01N21/33
Inventor 尹东光刘斌虎张礼谢春娟张乐
Owner SHANGHAI UNIV
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