Near-infrared second region fluorescent nanoprobe based on black phosphorus as well as preparation and application of near-infrared second region fluorescent nanoprobe

A fluorescent nanoprobe and near-infrared technology, applied in the field of biomedicine, can solve the problems of less research on fluorescent properties, and achieve the effects of easy promotion, high yield, and broad application prospects

Active Publication Date: 2019-06-21
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because its luminescent properties are affected by the surrounding environment (such as water molecules, oxygen), there are relatively few studies on the fluorescence properties of black phosphorus nanoparticles solution, especially there is no report on the fluorescence properties of its near-infrared second region in the field of biomedicine

Method used

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  • Near-infrared second region fluorescent nanoprobe based on black phosphorus as well as preparation and application of near-infrared second region fluorescent nanoprobe
  • Near-infrared second region fluorescent nanoprobe based on black phosphorus as well as preparation and application of near-infrared second region fluorescent nanoprobe
  • Near-infrared second region fluorescent nanoprobe based on black phosphorus as well as preparation and application of near-infrared second region fluorescent nanoprobe

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Weigh 1.00g of red phosphorus into a 100mL stainless steel ball mill jar, add stainless steel beads (the mass ratio of red phosphorus to ball mill beads is 1:150) to seal, fix the ball mill jar in the ball mill, and then ball mill at a speed of 500r / min for 96h , to obtain black phosphorus nanoparticles powder. The X-ray diffraction patterns (XRD) of raw material red phosphorus (RP) and product black phosphorus (BP) are shown in figure 1 .

Embodiment 2

[0049] 4 g of oleylamine (molecular weight = 267 Da) was added to the black phosphorus nanoparticle powder prepared in Example 1, and ball milling was continued for 72 h. The obtained product was dispersed in tetrahydrofuran and centrifuged at a speed of 1000 r / min to remove the precipitate. An appropriate amount of ethanol was added as a precipitating agent to aggregate black phosphorus-oleylamine (BP-OM) nanoparticles to form insoluble matter, and then centrifuged at a speed of 5000r / min for 5min, and the precipitate was collected and redissolved in tetrahydrofuran. The obtained solution was centrifuged again at a speed of 1000 r / min, and the supernatant, namely the purified BP-OM nanoparticles, was taken. figure 2 It is a particle size distribution diagram of BP-OM nanoparticles in tetrahydrofuran, and its average particle size is 21nm. image 3 It is the transmission electron microscope picture of BP-OM, and the statistical average particle size is 14nm.

[0050] Take t...

Embodiment 3

[0052] Disperse 50mg of phospholipid-polyethylene glycol (lipid-PEG) molecules (the molecular weight of the PEG segment is 2000Da) in the tetrahydrofuran solution of the BP-OM nanoparticles prepared in Example 2. After the tetrahydrofuran is completely volatilized at room temperature, add ultrapure water Stir vigorously for 1 min. Then centrifuge at a speed of 3000r / min for 10min to remove insoluble matter, and dialyze the obtained supernatant to remove excess lipid-PEG. The molecular weight cut-off of the dialysis bag is 8000-100,000Da. After freeze-drying, the OM and lipid-PEG double-modified black phosphorus nanoparticles powder that can be stored for a long time was obtained, which was named BP-OM@lipid-PEG nanoparticles. Figure 5 It is the hydrated particle size distribution diagram of BP-OM@lipid-PEG nanoparticle aqueous solution, and its average hydrated particle size is 120nm. Figure 6 It is a transmission electron microscope picture of BP-OM@lipid-PEG nanoparticles...

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Abstract

The invention relates to a near-infrared second region fluorescent nanoprobe based on black phosphorus as well as a preparation and application of the near-infrared second region fluorescent nanoprobe. The preparation method of the near-infrared second region fluorescent nanoprobe comprises the following steps: uniformly mixing red phosphorus or black phosphorus with a ball-milling body and then carrying out ball milling for 1 to 200 hours; then adding a hydrophobic ligand into a mixture and continuously carrying out ball milling for 1 to 200 hours to obtain black phosphorus nanoparticles, thesurfaces of which are modified with the hydrophobic ligand; dissolving the black phosphorus nanoparticles modified with the hydrophobic ligand on the surface and the amphipathic molecules into a volatile organic solvent according to the mass ratio of 1 to (100 to 200); then volatilizing to remove the organic solvent, uniformly mixing the obtained substance with water and vigorously stirring to obtain water-soluble near-infrared second region fluorescent nanoprobe based on the black phosphorus. The near-infrared second region fluorescent nanoprobe obtained by the preparation method disclosed by the invention has the advantages of stronger and wider fluorescent signal, capability of achieving multi-wavelength excitation and multi-wavelength emission and broad application prospect in biological imaging.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a black phosphorus-based fluorescent nanoprobe in the second near-infrared region and its preparation and application. Background technique [0002] As an emerging two-dimensional semiconductor material, black phosphorus has unique photoelectric properties (high carrier mobility, tunable bandgap, high switching rate) and photothermal effect with its single-layer or several-layer nanostructure. Reserves and biomedicine have attracted a lot of attention. In biomedicine, black phosphorus and its degradation products are considered to have excellent biocompatibility because phosphorus is an essential element for biological organisms and functions. However, black phosphorus nanostructures are easily degraded by the interaction with water and oxygen, which affects its optical and electrical properties and application performance. Therefore, how to improve its optical and electrical propert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/70B82Y20/00B82Y30/00G01N21/64
Inventor 李桢徐一帆赵崇军
Owner SUZHOU UNIV
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