The invention provides a heterogeneous core-shell double-
enzyme cascade reactor as well as a preparation method and application thereof, and belongs to the technical field of sensing or
photocatalysis. The preparation method of the heterogeneous core-shell double-
enzyme cascade reactor comprises the following steps: mixing and incubating a first
enzyme and a carboxylated
metal organic framework in a room-temperature water phase, and washing to obtain a
metal organic framework heterogeneous
seed crystal loaded with the first enzyme; the heterogeneous
seed crystal is dispersed in an
aqueous solution of an amine
monomer for pre-
assembly, an
aldehyde monomer, a second enzyme and an
ionic liquid catalyst are added into the obtained
system, a reaction is performed at
room temperature, after the reaction is finished, a
solid is separated and washed, and the heterogeneous core-shell double-enzyme
cascade reactor is obtained. The invention aims to realize
high activity maintenance of enzyme in a heterogeneous framework through a mild and biocompatible interface-oriented
assembly strategy, solve the problems of a single porous material in
mass transfer regulation and
structural stability, and significantly improve
cascade reaction efficiency and operation stability.