Preparation method of fluorescence probe and applications of the fluorescence probe

A fluorescent probe and probe technology, applied in biochemical equipment and methods, DNA preparation, fluorescence/phosphorescence, etc., can solve the problems of fast photobleaching, easy decomposition, short fluorescence lifetime, etc., and achieve strong fluorescent signal and selectivity Strong, water-soluble effect

Inactive Publication Date: 2014-06-04
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, all kinds of markers currently used in biology have shortcomings, such as fluorescent organic dye molecules are generally more toxic, poor biocompatibility, fast photobleaching, unstable, prone to fluorescence quenching, fluorescence Short life; semiconductor quantum dots have certain biological toxicity due to their heavy metal ions, and are easy to decompose under high concentration conditions or when exposed to ultraviolet radiation, etc.
The shortcomings of these materials limit their applications in biomolecules, cell labeling, drug tracking, disease diagnosis, and fluorescence sensing.

Method used

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  • Preparation method of fluorescence probe and applications of the fluorescence probe
  • Preparation method of fluorescence probe and applications of the fluorescence probe
  • Preparation method of fluorescence probe and applications of the fluorescence probe

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Embodiment 1

[0032] Embodiment 1: A preparation method of a fluorescent probe, the probe is obtained by connecting single-stranded DNA with reduced carbon quantum dots capable of emitting blue fluorescence, and the reduced carbon quantum dots are made of carbon quantum dots as raw materials, boron Sodium hydride is used as a reducing agent, and a chemical reduction method is used to modify the surface of the carbon quantum dots to obtain reduced carbon quantum dots capable of emitting blue fluorescence. The specific steps are:

[0033] A. Synthesis of carbon quantum dots

[0034] Add 0.3g of graphite powder into a mixed solution of 180mL of concentrated sulfuric acid and 60mL of concentrated nitric acid, ultrasonicate for 2 hours, heat, stir and reflux at 80°C for 24 hours, after the reaction is completed, cool to room temperature and dilute to 800mL with distilled water, and adjust the obtained brown-black solution with sodium carbonate The pH value is neutral, and a brown solution is ob...

Embodiment 2

[0042] Embodiment 2: A preparation method of a fluorescent probe, the probe is obtained by connecting single-stranded DNA with reduced carbon quantum dots capable of emitting blue fluorescence, and the reduced carbon quantum dots are made of carbon quantum dots as raw materials, boron Sodium hydride is used as a reducing agent, and a chemical reduction method is used to modify the surface of the carbon quantum dots to obtain reduced carbon quantum dots capable of emitting blue fluorescence. The specific steps are:

[0043] A. Synthesis of carbon quantum dots

[0044] Add 0.1g of graphite powder to 120mL of concentrated sulfuric acid and 40mL of concentrated nitric acid mixed solution, ultrasonic for 1 hour, heat and stir at 60°C and reflux for 22 hours, after the reaction is completed, cool to room temperature and dilute to 600mL with distilled water, and adjust the obtained brown-black solution with sodium carbonate The pH value is neutral, and a brown solution is obtained. ...

Embodiment 3

[0052] Embodiment 3: A preparation method of a fluorescent probe, the probe is obtained by connecting single-stranded DNA with reduced carbon quantum dots capable of emitting blue fluorescence, and the reduced carbon quantum dots are made of carbon quantum dots as raw materials, boron Sodium hydride is used as a reducing agent, and a chemical reduction method is used to modify the surface of the carbon quantum dots to obtain reduced carbon quantum dots capable of emitting blue fluorescence. The specific steps are:

[0053] A. Synthesis of carbon quantum dots

[0054] Add 0.55g of graphite powder to 240mL of concentrated sulfuric acid and 80mL of concentrated nitric acid mixed solution, ultrasonic for 3 hours, heat, stir and reflux at 100°C for 26 hours, after the reaction is completed, cool to room temperature and dilute to 1000mL with distilled water, and adjust the obtained brown-black solution with sodium carbonate The pH value is neutral, and a brown solution is obtained....

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Abstract

The invention relates to a fluorescence nano-material, and particularly relates to a preparation method of a fluorescence probe and applications of the fluorescence probe. The fluorescence probe has characteristics of strong fluorescence signals, high stability and good water solubility. The fluorescence probe is prepared by connecting reduced carbon quantum dots capable of emitting blue fluorescence to single-stranded DNA. The reduced carbon quantum dots are prepared from carbon quantum dots, and are prepare by subjecting the carbon quantum dots to surface modification by adopting sodium borohydride as a reductant and adopting a chemical reduction method so as to obtain the reduced carbon quantum dots capable of emitting the blue fluorescence. Detection of the target DNA is achieved by utilization of fluorescence quenching-recovery processes of the fluorescence probe.

Description

technical field [0001] The invention relates to a fluorescent nanometer material, in particular to a method for preparing a fluorescent probe and its application. Background technique [0002] Fluorescent labeling refers to the binding of fluorescent substances to the biomolecules to be studied, and the qualitative and quantitative determination of the target biomolecules by using the special fluorescent signals generated by the fluorescent markers. In recent years, fluorescent labeling technology has played an important role in protein, nucleic acid, cell detection and immune analysis. However, all kinds of markers currently used in biology have shortcomings, such as fluorescent organic dye molecules are generally more toxic, poor biocompatibility, fast photobleaching, unstable, prone to fluorescence quenching, fluorescence Short life; semiconductor quantum dots have certain biological toxicity due to their heavy metal ions, and are easy to decompose under high concentrati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10G01N21/64
CPCC12Q1/6818C12Q2563/107
Inventor 钱兆生单晓月丰慧柴鲁静马娟娟
Owner ZHEJIANG NORMAL UNIVERSITY
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