37results about How to "Realize cascade amplification" patented technology

The invention discloses a method for enriching and separating human peripheral blood CD34<+> and CD91<+> stem cells, which provides basis for the subsequent researches on CD34<+> and CD91<+> stem cells better, and relates to the field of biomedicine. The method comprises the following steps of: covalently coupling a multi-arm well star-shaped polymer with a mouse anti-human CD34<+> or CD91<+> monoclonal antibody; coating long-chain biotin molecules on the multi-arm well star-shaped polymer modified by the mouse anti-human CD34<+> or CD91<+> monoclonal antibody; capturing the CD34<+> and CD91<+> stem cells in a peripheral blood sample by the multi-arm well star-shaped polymer modified by the mouse anti-human CD34<+> or CD91<+> monoclonal antibody; identifying and coupling the long-chain biotinylated well star-shaped polymer in the peripheral blood by nanometre magnetic beads modified by streptavidin; separating and re-suspending the captured CD34<+> and CD91<+> stem cells, and the like, wherein the re-suspension can be directly used for subsequent analysis. Compared with the traditional cell separation method, the method is more suitable for performing magnetic separation on the CD34<+> and CD91<+> stem cells in the complex peripheral blood sample, and capable of increasing the separation efficiency for the CD34<+> and CD91<+> stem cells in the peripheral blood sample.

The invention discloses a method for separating and enriching natural killer cells (natural killer cells, NK) in peripheral blood for further providing a basis for subsequent study of natural killer cells, and relates to the biomedical field. The method comprises the following steps of: covalent coupling of dendritic polymers and mouse anti-human NKp46 antibodies; further coating long-chain biotin molecules by dendritic polymers modified by mouse anti-human NKp46 antibodies; capturing NK in a peripheral blood sample by dendritic polymers co-modified by mouse anti-human NKp46 antibodies and long-chain biotin; identifying and coupling long-chain dendritic polymers in the peripheral blood by streptavidin modified nanomagnetic beads; and separating and resuspending the captured NK. The resuspension can be directly used for subsequent analysis. Compared with conventional cell separation methods, the method is more suitable for magnetic separation of NK in complex peripheral blood samples, so that the magnetic separation time is shortened, and the NK separation efficiency in the peripheral blood sample is improved.

The invention discloses a method for separating and enriching dendritic cells (Dendritice cells, DC) in whole blood for further providing a basis for subsequent study of dendritic cells, and relates to the biomedical field. The method comprises the following steps of: covalent coupling of hyperbranched polymers and mouse anti-human dendritic cell monoclonal antibodies; further coating long-chain biotin molecules by hyperbranched polymers modified by mouse anti-human dendritic cell monoclonal antibodies; capturing DC in a whole blood sample by hyperbranched polymers co-modified by mouse anti-human dendritic cell monoclonal antibodies and long-chain biotin; identifying and coupling long-chain biotin hyperbranched polymers in the whole blood by streptavidin modified nanomagnetic beads; and separating and resuspending the captured DC. The resuspension can be directly used for subsequent analysis. Compared with conventional cell separation methods, the method is more suitable for magnetic separation of DC in complex whole blood samples, so that the magnetic separation time is shortened, and the DC separation efficiency in the whole blood sample is improved.

The invention discloses a method of enrichment separation of meticillin-resistant Sta-phylococcusaureus (MRSA), provides a better basic for follow-up study of target bacteria, and relates to the field of biology technology. The method comprises steps of: covalently coupling broom molecules and antibodies, coating antibody-modified broom molecules with long-chain biotin molecules, capturing target bacteria in a sample solution via broom molecules that are modified by the antibodies and the long-chain biotin molecules, identifying streptavidin-modified nano-magnetic-beads and coupling with long-chain biotinylated broom molecules in the sample solution, separating and re-suspending the captured bacteria. Because the re-suspending solution can be used for follow-up analyses directly, the method provide by the invention is even more suitable for magnetic separation of bacteria in complex matrixes compared with conventional magnetic separation of bacteria, and rises the separating efficiency of target bacteria in samples.

The invention discloses a method for enrichment separation of mycobacterium tuberculosis (MT), and better provides a basis to the follow-up study of target bacteria, relating to the field of biotechnology. The method comprises the following steps of: performing covalent coupling of a hyperbranched polymer and an antibody; coating a long-chain biotin molecule with the antibody-modified hyperbranched polymer; capturing target bacteria in sample liquid by use of the hyperbranched polymer co-modified by the antibody and the long-chain biotin; identifying streptavidin-modified nano magnetic beads, and coupling with the long-chain biotinylation hyperbranched polymer in the sample liquid; separating and re-suspending the captured bacteria, and the like, wherein the re-suspension liquid can be directly subjected to follow-up analysis. Compared with a traditional magnetic bacterium separation method, the method disclosed by the invention is more suitable for the magnetic separation of bacteria in a complicate matrix, and improves the separation efficiency of target bacteria in a sample.

The invention discloses a method for enriching and separating staphylococcus aureus (SA), provides a good basis to the subsequent research on a target bacterium, and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a dendrimer and an antibody; coating the antibody modified dendrimer with a long-chain biotin molecule; capturing a target bacterium in a sample liquid by the antibody and long-chain biotin comodified dendrimer; identifying and coupling the long-chain biotin dendrimer in the sample liquid by using streptavidin modified nano-magnetic beads; and carrying out separation and weight suspending on the captured bacterium, wherein the weight suspending solution can be directly used for subsequent analysis. Compared with a traditional bacterium magnetic separation method, the method is more suitable for magnetically separating bacterium in a complex matrix, so that the target bacterium separation efficiency in a sample can be improved.