The invention discloses a method for separating hematopoietic stem cells (HSC) enriched in
peripheral blood, aims to better supply subsequent research on the HSC, and relates to the field of
biomedicine. The method comprises the following steps of: performing covalent
coupling on dendritic
hyperbranched polymers and anti-rat human HSC
monoclonal antibodies, wrapping the dendritic
hyperbranched polymers modified by the anti-rat human HSC
monoclonal antibodies by long-chain
biotin molecules, capturing the HSC in a
peripheral blood sample by the dendritic
hyperbranched polymers co-modified by the anti-rat human HSC
monoclonal antibodies and the long-chain biotins, identifying nano magnetic beads modified by
streptavidin, making the nano magnetic beads be coupled with the long-chain
biotin dendritic hyperbranched polymers in the
peripheral blood, separating the captured HSC, performing heavy suspension on the captured HSC and the like, wherein heavy suspension liquid can be directly subjected to subsequent analysis. Compared with the conventional
cell separation method, the method disclosed by the invention is more suitable for performing
magnetic separation on the HSC in the complicated
peripheral blood sample and the efficiency of separating the HSC from the
peripheral blood sample is improved.