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37results about How to "Realize cascade amplification" patented technology
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Separation method of micro circulating tumor cells
InactiveCN103275934AEasy to separateImprove separation efficiencyTumor/cancer cellsDendrimerCell separation
The invention discloses a method for separating and enriching micro circulating tumor cells (circulating tumor cells, CTCs) in whole blood for further providing a basis for subsequent study of tumor cells, and relates to the biomedical field. The method comprises the following steps of: covalent coupling of dendrimers and matters (special matters) specifically combined with target cells; further coating long-chain biotin molecules by dendrimers modified by the specific matters; capturing CTCs in a whole blood sample by dendrimers co-modified by the special matters and the long-chain biotin; identifying and coupling long-chain biotin dendrimers in the whole blood by streptavidin modified nanomagnetic beads; and separating and resuspending the captured CTCs. The resuspension can be directly used for subsequent analysis. Compared with conventional cell separation methods, the method is more suitable for magnetic separation of CTCs in complex whole blood samples, so that the magnetic separation time is shortened, and the CTCs separation efficiency in the whole blood sample is improved.
Owner:NANCHANG UNIV
Method for directly separating CD<4+> and CD<8+> lymphocytes
ActiveCN103305464AImprove separation efficiencyThe reaction solution is stableBlood/immune system cellsBiotinylationBiotin-streptavidin complex
The invention discloses a method for directly separating CD<4+> and CD<8+> lymphocytes, lays a better foundation for the subsequent research on the CD<4+> and CD<8+> lymphocytes, and relates to the field of biomedicines. The method comprises steps of: multi-arm well and star-shaped polymer and mouse anti-human CD<4+> or CD<8+> monoclonal antibody covalent coupling, long-chain biotin molecule coating through utilizing a mouse anti-human CD<4+> or CD<8+> monoclonal antibody-modified multi-arm well and star-shaped polymer, peripheral blood sample CD<4+> and CD<8+> lymphocyte acquiring through utilizing a mouse anti-human CD<4+> or CD<8+> monoclonal antibody and long-chain biotin co-modified multi-arm well and star-shaped polymer, peripheral blood long-chain biotinylation multi-arm well and star-shaped polymer identifying and coupling through utilizing streptavidin-modified nano magnetic beads, captured CD<4+> and CD<8+> lymphocyte separating and suspending and the like. A suspension can be directly used for subsequent analysis; and compared with a conventional cell separating method, the method is suitable for magnetically separating complicated peripheral blood sample CD<4+> and CD<8+> lymphocytes, so that the peripheral blood sample CD<4+> and CD<8+> lymphocyte separation efficiency is increased.
Owner:NANCHANG UNIV
Fluorescent biological probe for detecting mucin 1, sensor, application and detection method
ActiveCN111351944AHigh detection sensitivityStrong specificityBiological material analysisBiological testingChain reactionBiological organism
The invention relates to the technical field of mucin 1 detection, in particular to a fluorescent biological probe for mucin 1 detection, a sensor, application and a detection method. In the present invention, the aptamer probe is used for an Exo I assisted target circulation (EATR) reaction. The hairpin probe comprises a hairpin probe H1 and a hairpin probe H2 which are used for GO-assisted hybridization chain reaction (HCR); the aptamer probe and the hairpin probe are combined to be used for mucin 1 detection, so organic combination of Exo I-assisted target circulation reaction and GO-assisted hybridization chain reaction can be realized; therefore, cascade amplification of fluorescence signals is realized, the detection sensitivity of mucoprotein 1 is remarkably improved, the detectionlimit is reduced, quantitative detection of low-concentration mucin 1 can be realized, and theprobe has high specificity and can be used for specifically identifying and detecting mucin 1.
Owner:JIANGSU INST OF NUCLEAR MEDICINE
Immunoassay method based on fluorescein and luciferase bioluminescent reaction and application thereof
InactiveCN105467113ARealize cascade amplificationLuminous reaction is fastMaterial analysisFluoresceinLuciferase Gene
The invention provides an immunoassay method based on fluorescein and luciferase bioluminescent reaction and application thereof. The method comprises the steps of: (a) coating a coated material on a solid carrier, incubating and washing with a buffer; (2) adding a determinand standard solution, incubating and washing with a buffer; (3) adding ALP labeled secondary antibody which is an anti-determinand antibody, incubating and washing with a buffer; (4) adding an ATP solution, reacting, adding a bio-luminescent substrate, reacting, and finally testing a luminescence value to obtained a result I; (5) replacing the determinand standard solution in the step (2) with a determinand sample solution, repeating the operations in the step (1) to (4), detecting a luminescence value to obtain a result II, comparing the result II with the result I, and conversing to obtain the concentration of the determinand in the sample solution. The immunoassay method has the advantages of high sensitivity, simple signal readout mode, wide linear range, and fast analysis.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Method for detecting pesticide residues by using magnetic relaxation time sensor based on quantity and state change of nano magnetic particles
ActiveCN111007252ARealize cascade amplificationHigh sensitivityMagnetic immunoreagent carriersPesticide residueNanoparticle
The invention discloses a method for changing the number and state of nano magnetic particles based on a biological orthogonal reaction for detecting pesticide residues. According to the method, the number and the aggregation state of the nano magnetic particles are changed simultaneously on the basis of the cascade biological orthogonal reaction of diphenyl cyclooctyne and azide, controllable adjustment of the number and the state of the magnetic nano particles is realized, and magnetic signal cascade amplification is performed by organic combination of diphenyl cyclooctyne and azide, so thatthe accuracy and the sensitivity of pesticide residue detection are improved.
Owner:WUHAN ACADEMY OF AGRI SCI +1
Novel quick separation method of bacilluscereus
InactiveCN103289929AAchieve separationEasy to separateBacteriaMicroorganism based processesBiotin-streptavidin complexBiotechnology
The invention discloses a novel quick separation method of bacilluscereus, which provides the basis for the subsequent research for a target bacterium and relates to the technical field of biology. The novel quick separation method comprises the steps of: carrying out covalent coupling on a multi-arm star-shaped polymer and an antibody, coating the antibody-modified multi-arc star-shaped polymer on a long-chain biotin molecule, capturing the target bacterium in a sample solution by the antibody and long-chain biotin co-modified multi-arc star-shaped polymer, recognizing and coupling the long-chain biotin multi-arm star-shaped polymer in the sample solution by a streptavidin-modified nano magnetic bead, separating and heavily-suspending the captured bacterium; a heavily-suspended liquid can be directly analyzed in subsequent. Compared with a conventional bacterium magnetic separation method, the novel quick separation method is more suitable for carrying out magnetic separation on the bacterium in a complex matrix; the separation efficiency of the target bacterium in a sample is increased.
Owner:NANCHANG UNIV
Efficient method for separating Campylobacter jejuni
InactiveCN103320422AEasy to separateImprove separation efficiencyBacteriaMicroorganism based processesBiotechnologyMagnetic bead
The invention discloses a method of enrichment separation of Campylobacter jejuni, provides a better basic for follow-up study of target bacteria, and relates to the field of biology technology. The method comprises steps of: covalently coupling broom molecules and antibodies, coating antibody-modified broom molecules with long-chain biotin molecules, capturing target bacteria in a sample solution via broom molecules that are modified by the antibodies and the long-chain biotin molecules, identifying streptavidin-modified nano-magnetic-beads and coupling with long-chain biotinylated broom molecules in the sample solution, separating and re-suspending the captured bacteria. Because the re-suspending solution can be used for follow-up analyses directly, the method provide by the invention is even more suitable for magnetic separation of bacteria in complex matrixes compared with conventional magnetic separation of bacteria, and rises the separating efficiency of target bacteria in samples.
Owner:鹰潭市金掌柜食品有限公司
Method for enriching and separating helicobacter pylori
InactiveCN103275902AAchieve separationEasy to separateBacteriaMicroorganism based processesBacteroidesBiotin-streptavidin complex
The invention discloses a method for enriching and separating helicobacter pylori (Helicobacter pylori, Hp), provides a basis for subsequent research on target bacteria and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a hyperbranched polymer and an antibody, coating a long-chain biotin molecule on the antibody-modified hyperbranched polymer, capturing the target bacteria in a sample solution through the dendriform hyperbranched polymer modified by the antibody and the long-chain biotin, identifying streptavidin-modified nano magnetic beads and coupling the long-chain biotin dendriform hyperbranched polymer in the sample solution, separating and suspending the captured bacteria, wherein suspension can be directly analyzed later. Compared with the traditional bacteria magnetic separation method, the method is suitable for performing magnetic separation on the bacteria in a complex substrate, and the separation efficiency of target bacteria in the sample is improved.
Owner:NANCHANG UNIV
Method for enriching and separating listeria monocytogenes
InactiveCN103275903AAchieve separationEasy to separateBacteriaMicroorganism based processesDendrimerBacteroides
The invention discloses a method for enriching and separating listeria monocytogenes (Listeria monocytogenes, Lm), provides a basis for subsequent research on target bacteria and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a dendrimer and an antibody, coating a long-chain biotin molecule on the antibody-modified dendrimer, capturing the target bacteria in a sample solution through the dendrimer modified by the antibody and the long-chain biotin, identifying streptavidin-modified nano magnetic beads and coupling the long-chain biotin dendrimer in the sample solution, separating and suspending the captured bacteria, wherein suspension can be directly analyzed later. Compared with the traditional bacteria magnetic separation method, the method is suitable for performing magnetic separation on the bacteria in a complex substrate, and the separation efficiency of target bacteria in the sample is improved.
Owner:NANCHANG UNIV
Signal-amplification fluorescence detection system, fluorescence biosensor and application thereof
PendingCN111521592AQuick checkImprove accuracyFluorescence/phosphorescenceBiologic markerFluorescence biosensor
The invention discloses a signal-amplification fluorescence detection system which comprises a recognition unit, which is marked with fluorescent molecules, is used for recognizing biomarker moleculesof Alzheimer's disease, and is combined with the biomarker molecules to form a biological complex; a fluorescence quenching unit which is used for adsorbing the recognition unit to quench fluorescence of the fluorescent molecules, wherein the fluorescence quenching unit and the biological complex are desorbed, so that fluorescence of fluorescence molecules is recovered; and a signal amplificationunit, which is used for degrading the recognition unit in the biological complex. The fluorescence detection system is used for detecting the biomarker molecules of the Alzheimer's disease, the fluorescence detection system has the advantages of rapidness, simplicity, sensitivity and high efficiency; and meanwhile, the system can amplify a fluorescent molecular signal and reduce the detection limit of the system, and has high sensitivity. The invention further discloses a fluorescence biosensor which comprises the fluorescence detection system and can provide effective information for diagnosis and screening of the Alzheimer's disease.
Owner:SHENZHEN UNIV
Method for enriching and separating human peripheral blood CD34+ and CD91+ lymphocytes
InactiveCN103305462AAchieve separationSeparate applicationBlood/immune system cellsMagnetic beadCell separation
The invention discloses a method for enriching and separating human peripheral blood CD34<+> and CD91<+> stem cells, which provides basis for the subsequent researches on CD34<+> and CD91<+> stem cells better, and relates to the field of biomedicine. The method comprises the following steps of: covalently coupling a multi-arm well star-shaped polymer with a mouse anti-human CD34<+> or CD91<+> monoclonal antibody; coating long-chain biotin molecules on the multi-arm well star-shaped polymer modified by the mouse anti-human CD34<+> or CD91<+> monoclonal antibody; capturing the CD34<+> and CD91<+> stem cells in a peripheral blood sample by the multi-arm well star-shaped polymer modified by the mouse anti-human CD34<+> or CD91<+> monoclonal antibody; identifying and coupling the long-chain biotinylated well star-shaped polymer in the peripheral blood by nanometre magnetic beads modified by streptavidin; separating and re-suspending the captured CD34<+> and CD91<+> stem cells, and the like, wherein the re-suspension can be directly used for subsequent analysis. Compared with the traditional cell separation method, the method is more suitable for performing magnetic separation on the CD34<+> and CD91<+> stem cells in the complex peripheral blood sample, and capable of increasing the separation efficiency for the CD34<+> and CD91<+> stem cells in the peripheral blood sample.
Owner:北京佑仁生物科技集团有限公司 +1
Method for separating hematopoietic stem cells from human peripheral blood
ActiveCN103289954AAchieve separationSeparate applicationBlood/immune system cellsBiotin-streptavidin complexPolymer modified
The invention discloses a method for separating hematopoietic stem cells (HSC) enriched in peripheral blood, aims to better supply subsequent research on the HSC, and relates to the field of biomedicine. The method comprises the following steps of: performing covalent coupling on dendritic hyperbranched polymers and anti-rat human HSC monoclonal antibodies, wrapping the dendritic hyperbranched polymers modified by the anti-rat human HSC monoclonal antibodies by long-chain biotin molecules, capturing the HSC in a peripheral blood sample by the dendritic hyperbranched polymers co-modified by the anti-rat human HSC monoclonal antibodies and the long-chain biotins, identifying nano magnetic beads modified by streptavidin, making the nano magnetic beads be coupled with the long-chain biotin dendritic hyperbranched polymers in the peripheral blood, separating the captured HSC, performing heavy suspension on the captured HSC and the like, wherein heavy suspension liquid can be directly subjected to subsequent analysis. Compared with the conventional cell separation method, the method disclosed by the invention is more suitable for performing magnetic separation on the HSC in the complicated peripheral blood sample and the efficiency of separating the HSC from the peripheral blood sample is improved.
Owner:深圳市旷逸生物科技有限公司
Method for separating hematopoietic stem cells from human peripheral blood
ActiveCN103289954BAchieve separationSeparate applicationBlood/immune system cellsBiotin-streptavidin complexPolymer modified
The invention discloses a method for separating hematopoietic stem cells (HSC) enriched in peripheral blood, aims to better supply subsequent research on the HSC, and relates to the field of biomedicine. The method comprises the following steps of: performing covalent coupling on dendritic hyperbranched polymers and anti-rat human HSC monoclonal antibodies, wrapping the dendritic hyperbranched polymers modified by the anti-rat human HSC monoclonal antibodies by long-chain biotin molecules, capturing the HSC in a peripheral blood sample by the dendritic hyperbranched polymers co-modified by the anti-rat human HSC monoclonal antibodies and the long-chain biotins, identifying nano magnetic beads modified by streptavidin, making the nano magnetic beads be coupled with the long-chain biotin dendritic hyperbranched polymers in the peripheral blood, separating the captured HSC, performing heavy suspension on the captured HSC and the like, wherein heavy suspension liquid can be directly subjected to subsequent analysis. Compared with the conventional cell separation method, the method disclosed by the invention is more suitable for performing magnetic separation on the HSC in the complicated peripheral blood sample and the efficiency of separating the HSC from the peripheral blood sample is improved.
Owner:深圳市旷逸生物科技有限公司
Method for enriching and separating human peripheral blood CD34+ and CD91+ lymphocytes
InactiveCN103305462BAchieve separationSeparate applicationBlood/immune system cellsMagnetic beadCell separation
Owner:北京佑仁生物科技集团有限公司 +1
Probe set for detecting SARS-CoV-2 RNA, ECL biosensor and preparation method and application thereof
PendingCN113403369AQuick checkActivate trans-cleavage propertiesMicrobiological testing/measurementChemiluminescene/bioluminescenceElectron transferDNA
The invention provides a system for detecting SARS-CoV-2RNA. The system comprises a probe group, a CRISPR / Cas12a system and an ECL biosensor. A self-enhanced ruthenium compound is linked to the surface of ZIF-8, and high intramolecular electron transfer efficiency is obtained under the condition that a co-reactant is not added, so that a strong and stable ECL signal is obtained. The DSN participates in target circulation and CHA signal amplification, so that cascade amplification of signals is realized, and unstable target RNA is converted into stable dsDNA to be output. The dsDNA in the strategy can activate the trans-cleavage characteristic of Cas12a, and the Fc-labeled DNA probe which shows affinity with C3N4 is cleaved. Therefore, the concentration of the target RNA can determine the number of the Fc-labeled DNA probes adsorbed on the surface of C3N4, so that the ECL intensity is directly influenced.
Owner:JIANGSU INST OF NUCLEAR MEDICINE
Quick separation method of dendritic cells
InactiveCN103275932AAchieve separationEasy to separateBlood/immune system cellsBiotin-streptavidin complexPolymer modified
The invention discloses a method for separating and enriching dendritic cells (Dendritice cells, DC) in whole blood for further providing a basis for subsequent study of dendritic cells, and relates to the biomedical field. The method comprises the following steps of: covalent coupling of hyperbranched polymers and mouse anti-human dendritic cell monoclonal antibodies; further coating long-chain biotin molecules by hyperbranched polymers modified by mouse anti-human dendritic cell monoclonal antibodies; capturing DC in a whole blood sample by hyperbranched polymers co-modified by mouse anti-human dendritic cell monoclonal antibodies and long-chain biotin; identifying and coupling long-chain biotin hyperbranched polymers in the whole blood by streptavidin modified nanomagnetic beads; and separating and resuspending the captured DC. The resuspension can be directly used for subsequent analysis. Compared with conventional cell separation methods, the method is more suitable for magnetic separation of DC in complex whole blood samples, so that the magnetic separation time is shortened, and the DC separation efficiency in the whole blood sample is improved.
Owner:NANCHANG UNIV
Method for magnetically separating yersinia enterocolitica (YE)
InactiveCN103333817BEasy to separateImprove separation efficiencyBacteriaMicroorganism based processesBiotin-streptavidin complexDendrimer
The invention relates to the technical field of biology and discloses a method for enriching and separating yersinia enterocolitica (YE). The method comprises the following steps: performing covalent coupling on a dendrimer and an antibody; coating the antibody modified dendrimer with a long-chain biotin molecule; capturing a target bacterium in a sample liquid by the antibody and long-chain biotin comodified dendrimer; identifying and coupling the long-chain biotin dendrimer in the sample liquid by using streptavidin modified nano-magnetic beads; and carrying out separation and weight suspending on the captured bacterium, wherein the weight suspending solution can be directly used for subsequent analysis. Compared with a traditional bacterium magnetic separation method, the method is more suitable for magnetically separating bacteria in a complex matrix, so that the target bacterium separation efficiency in a sample can be improved.
Owner:鹰潭市金掌柜食品有限公司
High-efficiency rapid vibrio parahemolyticus enrichment and separation method
InactiveCN103305441BAchieve separationEasy to separateBacteriaMicroorganism based processesBiotin-streptavidin complexMagnetic bead
The invention discloses a high-efficiency rapid enrichment and separation method, mainly relates to a method for enriching and separating vibrio parahemolyticus from a matrix, aims at providing basis for follow-up study on target bacteria, and relates to the field of biotechnology. The method comprises the following steps of: carrying out covalent coupling on an arborescence hyper-branched polymer and an antibody, enveloping the antibody-modified arborescence hyper-branched polymer with long-chain biotin molecules, capturing the target bacteria in a sample liquid by using the arborescence hyper-branched polymer which is co-modified by the antibody and the long-chain biotin, recognizing and coupling the long-chain biotin arborescence hyper-branched polymer in the sample liquid by using streptavidin-modified nano magnetic beads, separating and heavy-suspending the captured bacteria, and the like. The captured bacteria can be subjected to follow-up analysis directly; and compared with the conventional bacterium separation method, the method disclosed by the invention is more applicable to magnetic separation of bacteria in a complex matrix, and the target bacterium separation efficiency in the sample is improved.
Owner:NANCHANG UNIV
Quick enriching and separating method of natural killer cells in peripheral blood of human
ActiveCN103275931BAchieve separationEasy to separateBlood/immune system cellsBiotin-streptavidin complexCoupling
Owner:深圳市旷逸生物科技有限公司
Separation method of Streptococcus pneumoniae in complex matrix
InactiveCN103275904BAchieve separationEasy to separateBacteriaMicroorganism based processesBiotechnologyMagnetic bead
The invention discloses a method for enriching and separating streptococcus pneumoniae in order to provide a basis for the follow-up study of target bacteria, relating to the field of biotechnology. The method comprises the steps of: covalent coupling between a dendritic polymer and an antibody, exposed amino of long-chain biotin blocking antibody-dendritic polymer, capturing of target bacteria in sample liquid by the antibody coupled with the dendritic polymer, identification of streptavidin coupled with nano magnetic beads and coupling with the long-chain biotinylated dendritic polymer in the sample liquid, magnetic separation and re-suspension of bacteria and the like. The re-suspension liquid can be directly subjected to follow-up analysis; and compared with the traditional magnetic bacterial separation method, the method disclosed by the invention is more suitable for the magnetic separation of bacteria in a complicated substrate, and improves the separation efficiency of the target bacteria in a sample.
Owner:NANCHANG UNIV
Method for directly separating CD<4+> and CD<8+> lymphocytes
InactiveCN103305464BAchieve separationSeparate applicationBlood/immune system cellsBiotin-streptavidin complexMagnetic bead
The invention discloses a method for directly separating CD<4+> and CD<8+> lymphocytes, lays a better foundation for the subsequent research on the CD<4+> and CD<8+> lymphocytes, and relates to the field of biomedicines. The method comprises steps of: multi-arm well and star-shaped polymer and mouse anti-human CD<4+> or CD<8+> monoclonal antibody covalent coupling, long-chain biotin molecule coating through utilizing a mouse anti-human CD<4+> or CD<8+> monoclonal antibody-modified multi-arm well and star-shaped polymer, peripheral blood sample CD<4+> and CD<8+> lymphocyte acquiring through utilizing a mouse anti-human CD<4+> or CD<8+> monoclonal antibody and long-chain biotin co-modified multi-arm well and star-shaped polymer, peripheral blood long-chain biotinylation multi-arm well and star-shaped polymer identifying and coupling through utilizing streptavidin-modified nano magnetic beads, captured CD<4+> and CD<8+> lymphocyte separating and suspending and the like. A suspension can be directly used for subsequent analysis; and compared with a conventional cell separating method, the method is suitable for magnetically separating complicated peripheral blood sample CD<4+> and CD<8+> lymphocytes, so that the peripheral blood sample CD<4+> and CD<8+> lymphocyte separation efficiency is increased.
Owner:NANCHANG UNIV
Magnetic enrichment and separation method of Shigellaspp in complex matrix
The invention discloses a magnetic enrichment and separation method of Shigellaspp in a complex matrix. The method provides the basis for the follow-up study on target bacteria and relates to the technical field of biology. The method comprises the following steps of: covalently coupling hyperbranched polymers with antibodies, enveloping long-chain biotin molecules by the hyperbranched polymers modified with the antibodies, capturing target bacteria in a sample solution by the hyperbranched polymers modified with the antibodies and long-chain biotin, identifying and coupling long-chain biotinylated hyperbranched polymers in the sample solution by nano magnetic beads modified with streptavidin, and carrying out separation and heavy suspension on the captured bacteria. The captured target bacteria can be directly used for the follow-up analysis. Compared with the traditional magnetic separation method of bacteria, the method is suitable for magnetic separation of the bacteria in the complex matrix, and the separation efficiency of the target bacteria in a sample is improved.
Owner:NANCHANG UNIV
Novel method for enriching and separating Candida albicans
InactiveCN103275879BAchieve separationEasy to separateFungiMicroorganism based processesMicrobiologyBiochemistry
The invention discloses a method for enrichment and separation, and mainly discloses a method for enriching and separating Candida albicans. The method provides a basis for better follow-up research on a target fungus, and relates to the technical field of biology. The method comprises the following steps that: a multi-arm star polymer is covalently coupled with an antibody, an antibody-modified multi-arm star polymer further coats long-chain biotin molecules, the multi-arm star polymer jointly modified by the antibody and the long-chain biotin molecules captures target bacteria in sample liquid, streptavidin-modified nano magnetic beads identify and couple with a long-chain biotinylated multi-arm star polymer in the sample liquid, the captured bacteria is separated and suspended again, and the like. The separated target bacteria can be directly subjected to follow-up analysis, and in comparison with a conventional bacteria separation method, the method disclosed by the invention is more suitable for performing magnetic separation on the bacteria in a complex ground substance, and the separation efficiency of the target bacteria in the sample is improved.
Owner:NANCHANG UNIV
Rapid Isolation Method of Dendritic Cells
InactiveCN103275932BAchieve separationEasy to separateBlood/immune system cellsBiotin-streptavidin complexPolymer modified
The invention discloses a method for separating and enriching dendritic cells (Dendritice cells, DC) in whole blood for further providing a basis for subsequent study of dendritic cells, and relates to the biomedical field. The method comprises the following steps of: covalent coupling of hyperbranched polymers and mouse anti-human dendritic cell monoclonal antibodies; further coating long-chain biotin molecules by hyperbranched polymers modified by mouse anti-human dendritic cell monoclonal antibodies; capturing DC in a whole blood sample by hyperbranched polymers co-modified by mouse anti-human dendritic cell monoclonal antibodies and long-chain biotin; identifying and coupling long-chain biotin hyperbranched polymers in the whole blood by streptavidin modified nanomagnetic beads; and separating and resuspending the captured DC. The resuspension can be directly used for subsequent analysis. Compared with conventional cell separation methods, the method is more suitable for magnetic separation of DC in complex whole blood samples, so that the magnetic separation time is shortened, and the DC separation efficiency in the whole blood sample is improved.
Owner:NANCHANG UNIV
Efficient method for separating meticillin-resistant Sta-phylococcusaureus
InactiveCN103320421BEasy to separateImprove separation efficiencyBacteriaMicroorganism based processesBiotin-streptavidin complexMagnetic bead
The invention discloses a method of enrichment separation of meticillin-resistant Sta-phylococcusaureus (MRSA), provides a better basic for follow-up study of target bacteria, and relates to the field of biology technology. The method comprises steps of: covalently coupling broom molecules and antibodies, coating antibody-modified broom molecules with long-chain biotin molecules, capturing target bacteria in a sample solution via broom molecules that are modified by the antibodies and the long-chain biotin molecules, identifying streptavidin-modified nano-magnetic-beads and coupling with long-chain biotinylated broom molecules in the sample solution, separating and re-suspending the captured bacteria. Because the re-suspending solution can be used for follow-up analyses directly, the method provide by the invention is even more suitable for magnetic separation of bacteria in complex matrixes compared with conventional magnetic separation of bacteria, and rises the separating efficiency of target bacteria in samples.
Owner:NANCHANG UNIV
Fast magnetic separation method of mycobacterium tuberculosis (MT)
InactiveCN103305440BAchieve separationEasy to separateBacteriaMicroorganism based processesBiotin-streptavidin complexMagnetic bead
The invention discloses a method for enrichment separation of mycobacterium tuberculosis (MT), and better provides a basis to the follow-up study of target bacteria, relating to the field of biotechnology. The method comprises the following steps of: performing covalent coupling of a hyperbranched polymer and an antibody; coating a long-chain biotin molecule with the antibody-modified hyperbranched polymer; capturing target bacteria in sample liquid by use of the hyperbranched polymer co-modified by the antibody and the long-chain biotin; identifying streptavidin-modified nano magnetic beads, and coupling with the long-chain biotinylation hyperbranched polymer in the sample liquid; separating and re-suspending the captured bacteria, and the like, wherein the re-suspension liquid can be directly subjected to follow-up analysis. Compared with a traditional magnetic bacterium separation method, the method disclosed by the invention is more suitable for the magnetic separation of bacteria in a complicate matrix, and improves the separation efficiency of target bacteria in a sample.
Owner:NANCHANG UNIV
Method for separating staphylococcus aureus (SA)
InactiveCN103333818BEasy to separateImprove separation efficiencyBacteriaMicroorganism based processesBiotin-streptavidin complexDendrimer
The invention discloses a method for enriching and separating staphylococcus aureus (SA), provides a good basis to the subsequent research on a target bacterium, and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a dendrimer and an antibody; coating the antibody modified dendrimer with a long-chain biotin molecule; capturing a target bacterium in a sample liquid by the antibody and long-chain biotin comodified dendrimer; identifying and coupling the long-chain biotin dendrimer in the sample liquid by using streptavidin modified nano-magnetic beads; and carrying out separation and weight suspending on the captured bacterium, wherein the weight suspending solution can be directly used for subsequent analysis. Compared with a traditional bacterium magnetic separation method, the method is more suitable for magnetically separating bacterium in a complex matrix, so that the target bacterium separation efficiency in a sample can be improved.
Owner:NANCHANG UNIV
Method for enriching and separating listeria monocytogenes
InactiveCN103275903BAchieve separationEasy to separateBacteriaMicroorganism based processesBacteroidesDendrimer
The invention discloses a method for enriching and separating listeria monocytogenes (Listeria monocytogenes, Lm), provides a basis for subsequent research on target bacteria and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a dendrimer and an antibody, coating a long-chain biotin molecule on the antibody-modified dendrimer, capturing the target bacteria in a sample solution through the dendrimer modified by the antibody and the long-chain biotin, identifying streptavidin-modified nano magnetic beads and coupling the long-chain biotin dendrimer in the sample solution, separating and suspending the captured bacteria, wherein suspension can be directly analyzed later. Compared with the traditional bacteria magnetic separation method, the method is suitable for performing magnetic separation on the bacteria in a complex substrate, and the separation efficiency of target bacteria in the sample is improved.
Owner:NANCHANG UNIV
Method for enriching and separating helicobacter pylori
InactiveCN103275902BAchieve separationEasy to separateBacteriaMicroorganism based processesBiotin-streptavidin complexBacteroides
The invention discloses a method for enriching and separating helicobacter pylori (Helicobacter pylori, Hp), provides a basis for subsequent research on target bacteria and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a hyperbranched polymer and an antibody, coating a long-chain biotin molecule on the antibody-modified hyperbranched polymer, capturing the target bacteria in a sample solution through the dendriform hyperbranched polymer modified by the antibody and the long-chain biotin, identifying streptavidin-modified nano magnetic beads and coupling the long-chain biotin dendriform hyperbranched polymer in the sample solution, separating and suspending the captured bacteria, wherein suspension can be directly analyzed later. Compared with the traditional bacteria magnetic separation method, the method is suitable for performing magnetic separation on the bacteria in a complex substrate, and the separation efficiency of target bacteria in the sample is improved.
Owner:NANCHANG UNIV
Method for separating streptococcus pneumoniae in complicated substrate
InactiveCN103275904AImprove separation efficiencyThe reaction solution is stableBacteriaMicroorganism based processesMolecular biologyBlocking antibody
The invention discloses a method for enriching and separating streptococcus pneumoniae in order to provide a basis for the follow-up study of target bacteria, relating to the field of biotechnology. The method comprises the steps of: covalent coupling between a dendritic polymer and an antibody, exposed amino of long-chain biotin blocking antibody-dendritic polymer, capturing of target bacteria in sample liquid by the antibody coupled with the dendritic polymer, identification of streptavidin coupled with nano magnetic beads and coupling with the long-chain biotinylated dendritic polymer in the sample liquid, magnetic separation and re-suspension of bacteria and the like. The re-suspension liquid can be directly subjected to follow-up analysis; and compared with the traditional magnetic bacterial separation method, the method disclosed by the invention is more suitable for the magnetic separation of bacteria in a complicated substrate, and improves the separation efficiency of the target bacteria in a sample.
Owner:NANCHANG UNIV
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