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Method for enriching and separating helicobacter pylori

A Helicobacter pylori enrichment technology, applied in the biological field, can solve the problems of separation failure, high concentration of miscellaneous bacteria, poor monodispersity of micron magnetic beads, etc., and achieve the effect of increasing contact opportunities, improving separation efficiency, and shortening separation time

Inactive Publication Date: 2015-03-11
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current separation technology based on micron-scale immunomagnetic beads has many limitations: 1) The specific surface area of ​​micron-sized magnetic beads is relatively small, which reduces the capture efficiency of magnetic beads; Bacterial cells are combined through a multiphase reaction (multiphase reaction), and it usually takes longer to specifically capture bacterial cells in the food matrix; 3) Micron magnetic beads have poor monodispersity and are prone to self-disruption in the food matrix solution. Aggregation or formation of precipitates; 4) Traditional immunomagnetic separation techniques often directly couple antibody molecules to immunomagnetic beads, which often leads to greatly reduced antibody activity and changes in the spatial direction of antibodies. The steric hindrance effect between antibodies reduces the capture efficiency of antibodies. 5) The nature of the food matrix is ​​complex and the concentration of non-target pathogenic bacteria is large. Micron magnetic beads are prone to non-specific adsorption, and it is difficult to realize the detection of food samples. Specific isolation of target bacteria; 6) Too high concentration of micron magnetic beads will cause damage to bacterial cells (the magnetic field causes the magnetic beads on the cell surface to attract each other, causing the cells to be squeezed or even ruptured, resulting in failure of separation

Method used

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  • Method for enriching and separating helicobacter pylori
  • Method for enriching and separating helicobacter pylori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. The dendritic hyperbranched polymer-antibody complex is prepared according to the following steps:

[0029] (1) Weigh 4.5 mg of dendritic polyamide-amine aminated by dendritic hyperbranched polymer, dissolve it in 4 mL of phosphate buffer (PBS, 0.01 mol / L, pH 8.0), stir and add 25% ammonium Dialdehyde aqueous solution 545 μL, so that the final concentration of glutaraldehyde is 3%. React at room temperature for 3.5 h at a rotating speed of 150 r / min on a shaker;

[0030] (2) Add Helicobacter pylori dropwise to the above solution HP Specific antibody 12 mg, so that the final concentration reached about 3 mg / mL. React at room temperature for 24 h at the speed of the shaker at 150 r / min;

[0031] (3) The above solution was spin-dried under reduced pressure, dissolved in deionized water, and dialyzed in PBS and deionized water for 1 day; after the dialysis, the obtained solution was freeze-dried.

[0032] 2. The long-chain biotin-dendritic hyperbranched ...

Embodiment 2

[0038] Example 2 Enrichment effect experiment

[0039] (1) Take 1 mL of concentration as 10 4 cfu / mL Helicobacter pylori in a 1.5 mL sterile centrifuge tube, centrifuge at 12000 rpm for 5 min, discard the supernatant, and resuspend with an equal volume of sterile PBS solution.

[0040] (2) Enrichment and capture: Set up the technical scheme group of the present invention (dendritic hyperbranched polymer group co-modified with Helicobacter pylori antibody and long-chain biotin), nano magnetic beads group modified with Helicobacter pylori specific antibody, and Helicobacter pylori Micron magnetic beads modified with bacteria-specific antibodies were used to enrich the target bacteria.

[0041] (3) After magnetic separation, pour the supernatant into a sterile centrifuge tube, and wash the isolated immunomagnetic beads with Helicobacter pylori captured twice with PBST, mix well, and resuspend with 1 mL sterile PBS solution Immunomagnetic bead complexes.

[0042] (4) Capture r...

Embodiment 3

[0055] Example 3 Enrichment capture experiment

[0056] Conventional magnetic stand separation time is 30min, and all the other are with embodiment 2.

[0057] The catch rate of each group is as follows:

[0058] Capture rate of Helicobacter pylori-specific antibody modified micron magnetic bead set Capture efficiency of magnetic nanobeads modified with specific antibodies against Helicobacter pylori Capture efficiency of dendritic hyperbranched polymer groups co-modified with Helicobacter pylori antibody and long-chain biotin 53.1% 34.8% 91.0%

[0059] The experimental results show that compared with the separation of 3 minutes in Example 2, when the separation time reaches 30 minutes, the capture efficiency of the three groups has been improved, especially the capture efficiency of the Helicobacter pylori-specific antibody-modified nano magnetic bead group is the most obvious. This shows that the capture efficiency of the nano-magnetic bead group...

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Abstract

The invention discloses a method for enriching and separating helicobacter pylori (Helicobacter pylori, Hp), provides a basis for subsequent research on target bacteria and relates to the technical field of biology. The method comprises the following steps: performing covalent coupling on a hyperbranched polymer and an antibody, coating a long-chain biotin molecule on the antibody-modified hyperbranched polymer, capturing the target bacteria in a sample solution through the dendriform hyperbranched polymer modified by the antibody and the long-chain biotin, identifying streptavidin-modified nano magnetic beads and coupling the long-chain biotin dendriform hyperbranched polymer in the sample solution, separating and suspending the captured bacteria, wherein suspension can be directly analyzed later. Compared with the traditional bacteria magnetic separation method, the method is suitable for performing magnetic separation on the bacteria in a complex substrate, and the separation efficiency of target bacteria in the sample is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for isolating food-borne pathogenic bacteria based on nano magnetic beads. Background technique [0002] Foodborne pathogen contamination is one of the major problems of food safety in my country. According to WHO statistics, about one-third of people in developed countries are infected with food-borne diseases every year, and 2.2 million people in the world die every year due to food-borne diseases. In my country, the number of cases of food poisoning is between 200,000 and 400,000 per year, most of which are caused by food-borne pathogens except for accidents. Helicobacter pylori (Helicobacter Pylor, HP) It is a microaerophilic bacterium, the ambient oxygen requirement is 5-8%, and it cannot survive in the atmosphere or an absolutely anaerobic environment, but it can be transmitted through hands, unclean food, unclean tableware, feces, etc. It is a chronic active gastrit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20G01N33/569C12R1/01
Inventor 魏华
Owner NANCHANG UNIV
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