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Electrochemiluminescence sensor based on functionalization metal organic frame material

A metal-organic framework and electrochemistry technology, applied in the direction of material electrochemical variables, scientific instruments, analytical materials, etc., can solve the problems of expensive analysis costs, limited application and popularization, limited technical sensitivity, etc., and achieve good stability, The effect of high sensitivity

Inactive Publication Date: 2018-01-05
UNIV OF JINAN
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Problems solved by technology

Although these technologies have development prospects, due to their high analysis costs and strict requirements for professional training of operators, this analysis technology can only be realized in large-scale laboratories with relatively complete conditions.
In addition, these techniques are limited in sensitivity and time-consuming, which also limits their application and popularization to some extent.
[0003] The development of microRNA detection methods is inseparable from new materials and technologies. Without breakthroughs in materials and technologies, it is difficult to make research progress in microRNA detection methods

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  • Electrochemiluminescence sensor based on functionalization metal organic frame material
  • Electrochemiluminescence sensor based on functionalization metal organic frame material

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Embodiment Construction

[0023] An electrochemiluminescent sensor based on functionalized metal-organic frameworks for the detection of MicroRNA-155

[0024] (1) The glassy carbon electrode (Ф = 3 mm) with a diameter of 3 mm was polished on 0.3 μm and 0.05 μm aluminum powder until a smooth surface was obtained, ultrasonically treated in 1:1 nitric acid and acetone, and washed with double distilled water , put the cleaned electrode in 0.5mol / L H 2 SO 4 The solution was scanned by cyclic voltammetry in the range of -0.8~1.5 V until it was stable, then cleaned with double distilled water, dried, and immersed in 5 mL of 0.1 mol / L HAuCl using an electrochemical workstation 4 In the solution, a layer of Au nanoparticles was formed on the surface of the glassy carbon electrode at a voltage of -0.2 V and electrodeposition for 40 s;

[0025] (2) Drop 10 μL of 5 μmol / L thiol-modified hairpin DNA-H1 strand on the electrode modified with Au nanoparticles, incubate at room temperature for 16 h, wash with double ...

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Abstract

The invention discloses an electrochemiluminescence sensor based on a functionalization metal organic frame material. The sensor utilizes Hg2+ to induce a 3(2,2'-dipyridyl) ruthenium functionalizationmetal organic frame material (RuMOFs) to be decomposed to release ECL of 3(2,2'-dipyridyl) ruthenium for detecting microRNA. Novel RuMOFs is prepared through a solvothermal method, can be selectivelyand sensitively decomposed by Hg2+ to release a large number of Ru(bpy)32+ and generate powerful ECL signals. Besides, to support a large number of RuMOFs, the target recycling process does not needthe help of nuclease, and the recycle can be achieved simply through a strand displacement process. Based on a decomposition and strand displacement process of an ion response material, a hypersensitive ECL method used for detecting microRNA is built. The method proves that in the detection range of 10 fmol / L-10 Mumol / L miRNA-155, the limit of detection is 3 fmol / L, wherein R=0.9961. The biosensorhas good analysis performance of being high in sensitivity, high in specificity, good in stability and the like, and provides a prospect for sensitivity detection of microRNA in clinical application.

Description

technical field [0001] The present invention relates to the detection method of microRNA, specifically a kind of using Hg 2+ Electrochemiluminescence (ECL) detection of microRNA by inducing tris(2,2'-bipyridyl)ruthenium functionalized metal-organic frameworks (RuMOFs) to decompose and release tris(2,2'-bipyridyl)ruthenium. Background technique [0002] microRNA is a large family of endogenous and small (about 18-25 nucleotides in length) non-coding RNA, which has new potential to be a novel biomarker for disease diagnosis or to simulate new molecules. Markers have aroused great interest among researchers. Sensitive and accurate detection of microRNA helps to understand diseases, can be used in the research and development of new drugs, and has great potential for treating human diseases. Currently, sensitive and quantitative detection methods for microRNA mainly include Northern analysis, blotting and quantitative real-time polymerase chain reaction. Although these techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
Inventor 葛慎光鉴燕楠兰飞飞王贺孙晓路梁琳琳颜梅于京华
Owner UNIV OF JINAN
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