53results about How to "Increase fluorescence signal intensity" patented technology

The invention relates to a nicking endonuclease-based netted rolling cycle amplification system and a use thereof. Based on the existing second-generation hyperbranched rolling cycle amplification technology, the amplification system utilizes a special endonuclease which is nicking endonuclease Nb.BsrDI so that a third-generation rolling cycle amplification technology which is a netted rolling cycle amplification (NGCA) technology is produced. Intensity of fluorescence signals produced by the netted rolling cycle amplification is increased with increasing of a target DNA concentration, is obviously stronger than that of fluorescence signals produced by linear rolling cycle amplification and hyperbranched rolling cycle amplification and has a detection limit of 0.1fM. Compared with the first-generation linear rolling cycle amplification and the second-generation hyperbranched rolling cycle amplification, the nicking endonuclease-based netted rolling cycle amplification system retains the prior art advantages in operationality, use cost and amplification time, further realizes signal amplification based on the prior art thereby providing good technical conditions for ultralow-abundance nucleic acid sample analysis and detection, and has a wide application prospect.

The invention relates to a composite micro-nano structure array on a high light-transmission substrate and a method and application of the same. The invention is characterized in that micro-nano structure arrays of light guide materials on the high light-transmission substrate are taken as supporting structures of fluorescent conjugated polymers for detection. The method comprises: firstly, preparing the micro-nano structure arrays and secondary structure arrays of the light guide materials on the high light-transmission material substrate such as quartz and the like; and secondly, coating the fluorescent conjugated polymers which are sensitive to specified analyte on the surface of the nano structure substrate to form the composite micro-nano structure array which is used for detecting an object. The invention takes the micro-nano structure arrays of different light guide materials as adhesive substrates of the fluorescent conjugated polymers and utilizes the advantages of micro-nanostructures such as large specific surface area, evanescent wave effect, micro resonant cavity action and the like to improve the sensitivity of detection and the signal intensity, prolong the servicelife and improve the reutilization property. The composite micro-nano structure array on the high light-transmission substrate can be applied to the detection of the specificity and high sensitivity of solid, liquid and gas molecules.

The invention discloses a circulating tumor cell identification kit and a circulating tumor cell identification method. The circulating tumor cell identification kit comprises a detection probe for detecting mRNA of an epithelial cell marking gene and / or mRNA of a mesenchymal cell marking gene, wherein the epithelial cell marking gene is selected from one or more of CDH2, VIMENTIN, FN1 and AKT2. Multiple RNA probes are adopted in the identification method, specific genes of a plurality of circulating tumor cells (CTCs) can be marked at the same time and are divided into genes I (epithelial genes), genes II (epithelial-mesenchymal genes) and genes III (mesenchymal genes), and false positive results caused by lack of part of the specific genes of the CTCs in a process that the CTCs enter peripheral blood for circulation are reduced; the method can be finished within eight hours, and a single copied mRNA hybridization probe is combined with a corresponding fluorescent probe by virtue of a signal amplification system, so that the detection sensitivity of in-situ RNA (Ribonucleic Acid) hybridization is remarkably improved.

The invention relates to a lung cancer related mciroRNA detection kit. The lung cancer related mciroRNA detection kit comprises: at least one first-grade signal amplification probe aiming at each type of lung cancer related mciroRNA to be detected, at least one second-grade signal amplification probe, at least one third-grade signal amplification probe and a capturing probe; and a P1 sequence of the capturing probe of the lung cancer related mciroRNA is selected from SEQ ID NO.1 to SEQ ID NO.21. The capturing probe and the signal amplification probes, selected by the invention, can be subjected to hybridization reaction under uniform reaction conditions, and all the probes are not in non-specifical combination; and the designed probes have good specificity and high signal-to-noise ratio. Meanwhile, a plurality of types of probes are combined so that a system with a complete detection effect is formed by utilizing an identification kit and a detection method.