The invention relates to an identification kit for circulating tumor cells of breast cancer. The identification kit comprises capturing probes, amplification probes and labeling probes aiming at marker gene mRNAs, wherein the marker gene mRNAs are selected from breast cancer cell marker gene mRNAs selected from at least two of HER2, SCGB2, MUC1 and MGB1, epithelial cell marker gene mRNAs selected from at least one of EpCAM, CDH1, KRT7, KRT8, KRT18 and KRT19, and mesenchymal cell marker gene mRNAs selected from at least one of FN1, CDH2, VIMNETIN, FOXC1 and TWIST1. With the identification kit, the false positive result caused by the factors including non-tumor epithelial cells in the blood and normal epithelial cells introduced in a sampling process is avoided, it is ensured that the cells in which the epithelial cell marker genes and/or mesenchymal cell marker genes are detected are circulating tumor cells of breast cancer, and thus the accuracy and reliability of the detection result are further improved.