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Digital signal processing method for detecting microfluidic chip and applied detection device

A digital signal processing and microfluidic chip technology, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve problems such as large step distortion, and achieve the effects of small distortion, reduced background noise, and good filter smoothness

Active Publication Date: 2011-11-23
BOAO BIOLOGICAL CO LTD +1
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Problems solved by technology

However, the step signal is composed of multiple harmonics in the frequency domain, and the narrower the passband means more higher harmonics are filtered out, the greater the step distortion

Method used

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  • Digital signal processing method for detecting microfluidic chip and applied detection device
  • Digital signal processing method for detecting microfluidic chip and applied detection device
  • Digital signal processing method for detecting microfluidic chip and applied detection device

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Embodiment Construction

[0058] The structure and effect of the present invention will be described in detail by citing the following embodiments in conjunction with the accompanying drawings.

[0059] The invention provides a portable microfluidic chip detection device, such as figure 1 As shown, it includes a microfluidic chip 10 , a bifocal plane imaging fluorescence detection system 20 , a signal processor 30 , a display screen 40 and a power supply 50 . in:

[0060] The microfluidic chip 10 includes one or more nanoliter-microliter volume microfluidic reaction chambers and connecting pipes, and the samples to be measured are placed in the reaction chambers with very little volume consumption. The fluorescence signal of the microfluidic chip 10 is input into the bifocal plane imaging fluorescence detection system 20 .

[0061] Dual-focal plane imaging fluorescence detection system 20 includes a light source 21, a beam splitter 22, an objective lens 23, an optical filter 24, an imaging lens 25, a...

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Abstract

The invention relates to a digital signal processing method for detecting a microfluidic chip and an applied detection device. The microfluidic chip detection device comprises the microfluidic chip, a bifocal surface imaging fluorescence detection system, a signal processor, a display screen and a power supply, wherein a fluorescence signal of the microfluidic chip is received by the bifocal surface imaging fluorescence detection system and then is transmitted to the signal processor; the signal processor is provided with a digital signal processing display software system; and a derivative slope fit movement average space-time simple filtering algorithm software module, an image analysis and calculation module, a data query module and a storage display module are preset in the digital signal processing display software system to process data input by the bifocal surface imaging fluorescence detection system. Therefore, stepped distortion of a digital signal acquired by the invention is low; the background noise is low; and the sensitivity of biochemical analysis and molecular diagnosis nucleic acid amplification real-time fluorescence signal detection is effectively improved.

Description

technical field [0001] The invention relates to microfluidic chip detection technology, in particular to a digital signal processing method for microfluidic chip detection and an applied detection device. Background technique [0002] Microfluidic chip technology or Micro-separation in Miniaturized Total Analysis System (μTAS: Micro-separation in Miniaturized Total Analysis System) is an emerging multidisciplinary interdisciplinary technology. , Mechanics, software and other system integration engineering technologies are organically cross-combined with multi-disciplinary application fields such as biology, analytical chemistry, and medicine. The continuous process is integrated into a small chip to realize automation, integration and portability from sample processing to result detection. Microfluidic chip detection instrument is a special instrument for the practical application of biochip technology. It usually uses microscopic magnification imaging, confocal scanning, e...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 王同舟黄国亮马丽罗贤波田浩黄杰黎新
Owner BOAO BIOLOGICAL CO LTD
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