Escherichia coli detection method based on time-resolved fluorescence method and DNA hybridization
A time-resolved fluorescence technology for Escherichia coli, which is applied in the field of microbial detection, can solve problems such as general constraints, cumbersome operation, and sensitivity limitations, and achieve the effects of reducing the probability of DNA degradation, simplifying the experimental process, and reducing pollutants
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[0036] The following is intended to illustrate the present invention without further limiting the present invention. The present invention can be implemented in any manner described in the summary of the invention.
[0037] 1. Design of capture probe and recognition probe
[0038] The DNA probe sequence of the present invention is based on published patents (Yao Zhijian, Yu Yong, Ma Liren, etc., gene chips, preparation methods, and kits for detecting a variety of common bacterial pathogens, Chinese patent, published on October 3, 2007, publication number CN101045945A ) The 16S rRNA gene conservative sequence of Escherichia coli was screened.
[0039] After literature search and sequence alignment in the National Center for Bioinformatics (NCBI) database, the target DNA sequence that is conserved and interspecific is 5'-CGT CCG ATC ACC TGC GTC AAT GTA ATG TTC TGC GAC GCTCAC ACC GAT AC-3'. DNA sequence complementary to the target sequence: 5'-GTA TCG GTG TGA GCG TCG CAG AACATT ACA TT...
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