4 results about "Escherichia coli detection" patented technology

This invention discloses a method and application for detecting F17 Escherichia coli. Using the fimbrial virulence gene ctg_04543 or ctg_04549 of F17 E. coli as a target, a fluorescent quantitative probe is designed based on the nucleotide sequence. Using F17 E. coli genomic DNA as a template, the F17 fimbrial gene ctg_04543 or ctg_04549 is detected by fluorescent quantitative PCR. Based on the fitted standard curve, F17 E. coli in the sample can be quantitatively detected. The F17 E. coli detection method provided by this invention is simple, rapid, quantitative, highly sensitive, and highly specific. It can achieve quantitative detection of F17 E. coli within 2-3 hours, meeting the detection needs of various situations such as laboratory research and animal pathogen diagnosis, and has broad application prospects and extremely high market implementation potential.

The present application relates to the technical field of microbial detection automation equipment, and discloses a pretreatment equipment for escherichia coli detection, which solves the problems proposed in the background art, and comprises a base, a filling station and a rotary driving assembly arranged on the base. The driving assembly drives the L-shaped workbench to rotate between the initial station and the processing station through the sleeve. The sleeve is provided with a bag opening clamping mechanism on the side, and the elastic adaptive module is used to fix the bag opening, which can effectively buffer the tension when the bag opening is folded. The horizontal part of the L-shaped workbench is provided with a bag body processing mechanism, which comprises a sealing clamp driven by a bidirectional screw and provided with a sealing convex rib and a groove, and an inertia beating assembly driven by a cylinder and capable of reciprocating. The beating assembly realizes soft beating through the inertia of the flexible beating plate. The present application simulates the "holding bag" and "beating" actions of human hands through the automatic mechanism, realizes the automatic filling, sealing and efficient beating mixing of the homogeneous bag, and improves the automation degree and processing efficiency of the pretreatment.

The application belongs to the technical field of biological detection, and particularly relates to a RPA primer for detecting Escherichia coli, a kit and application thereof. The target sequence is a new target DNA sequence which is broad-spectrum and specific in Escherichia coli. The RPA primer designed based on the target sequence can be used in recombinase polymerase isothermal amplification reaction of Escherichia coli. On this basis, the application provides a RPA detection method for detecting Escherichia coli by using the RPA primer. Compared with the existing PCR detection technology, the isothermal amplification detection method of the application can rapidly detect Escherichia coli at room temperature or under slight heating, and the detection can be completed within 30 min, thereby significantly improving the detection efficiency. Moreover, the detection method has high specificity, and the detection sensitivity can detect Escherichia coli as low as 10 1 copies / µL, and the specificity and sensitivity are much higher than those of the traditional PCR detection technology.

This invention relates to the field of biomedicine, specifically to an antibody for detecting Escherichia coli O45 and its application method. The antibody is a monoclonal antibody or its antigen-binding fragment, comprising the heavy chain CDR sequence shown in SEQ ID NO:1-3 and the light chain CDR sequence shown in SEQ ID NO:4-6. This invention also provides the nucleic acid molecule encoding the antibody, the expression vector, the host cell, and the preparation method thereof. Specificity identification results show that the antibody specifically binds to Escherichia coli O45 and shows no cross-reactivity with other Escherichia coli serotypes such as O157, O26, O103, O111, O121, and O145, as well as closely related serotypes of O55, and also shows no cross-reactivity with common foodborne pathogens. The indirect ELISA detection method based on this antibody has a detection limit of 1×10⁻⁶ for Escherichia coli O45. 2 The detection rate is CFU / mL, and the entire testing process can be completed within 24 hours. The monoclonal antibody provided by this invention can be used to prepare detection kits, test strips, and other products, and is suitable for rapid screening of O45 Escherichia coli in imported and exported food.