56 results about "Escherichia coli detection" patented technology

A presence / absence test for Staphylococcus aureus (S. aureus) involves placing a first generation test sample in a solution that will clot in the presence of S. aureus. The solution contains components that will selectively grow S. aureus and also contains clotting factors that will react with S. aureus, if S. aureus is present in the sample, to clot the solution. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, among others. The test can also be modified to detect the presence or absence of methicillin resistant S. Aureus (MRSA). The addition of micro particles having a size in the range of about 0.1 micron to about 1.0 mm provides localities where the bacteria agglomerate, thereby significantly decreasing the clotting time, and providing a significantly stronger clot. The micro particles can be used in other bacteria tests to accelerate the production of an end result. Such other tests can include a vancomycin-resistant enterococcus test; a Group B Streptococcus test; a test for hemolytic E. coli; and a test for Listeria monocytogenes, to name a few. These tests are all performed in a liquid broth-type reagent mixture and do not necessarily involve clotting of the broth.

The disclosed method simultaneously and specifically detects at least two types of food-poisoning bacteria from among Escherichia coli, Listeria, Campylobacter, Vibrio parahaemolyticus, Staphylococcus aureus, Salmonella, and Bacillus cereus. The food-poisoning bacteria detection carrier immobilizes one or at least two probes selected from each of at least two groups from among: an E. coli-detecting first probe group comprising the probes of sequence numbers 1-6 and probes (hereinafter written as "and the like") that are complementary thereto; a Listeria-detecting second probe group comprising the probes of sequence numbers 7-13, and the like; a Campylobacter-detecting third probe group comprising the probes of sequence numbers 14-19, and the like; a Vibrio parahaemolyticus-detecting fourth probe group comprising the probes of sequence numbers 20-24, and the like; a Staphylococcus aureus-detecting fifth probe group comprising the probes of sequence numbers 25-29, and the like; a Salmonella-detecting sixth probe group comprising the probes of sequence numbers 30-40, and the like; and a Bacillus cereus-detecting seventh probe group comprising the probes of sequence numbers 41-49.

A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.

The invention discloses a water quality genotoxicity detection method based on a semiconductor opening switch (SOS) effect of recombinant Escherichia coli. The method comprises the following steps of: recovering, activating and culturing recombinant Escherichia coli storage to obtain Escherichia coli detection solution; contacting a water sample to be measured with the detection solution, and simultaneously setting a contrast; centrifuging, ultrasonically disintegrating and performing other post-treatment on the contacted detection solution, and detecting a fluorescence intensity ratio of thewater sample to be measured; measuring a fluorescence intensity ratio of the Cr6+ solution with different concentrations, which is contacted with the detection solution by using the same method; drawing a standard curve between the fluorescence intensity ratio and the Cr6+ concentration, and substituting the fluorescence intensity ratio of the water sample to represent the water quality toxicity of the water sample through the Cr6+ concentration. The method has no pathogenic risk, is easy and convenient to culture and operate, high in detection sensitivity and low in cost; the final result isrepresented by Cr6+ equivalent concentration, and can be compared with results of other biological detection methods; and the method is suitable for rapidly detecting genotoxicity of an environmentalsample.