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Method for constructing multifunctional upconversion nano-platform

A construction method and a multifunctional technology, which can be applied to the construction of multifunctional up-conversion nanoplatforms, and can solve the problems of drug resistance, low resolution, and large toxic and side effects.

Inactive Publication Date: 2018-03-09
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, the early diagnosis and treatment of cancer in our country are facing the following problems: first, the early diagnosis methods used for tumor patients have poor sensitivity and low resolution; second, although radiotherapy and chemotherapy are the most commonly used methods in clinical Treatment method, but its toxic and side effects are large, and even lead to problems such as drug resistance in the body, affecting the therapeutic effect
Fluorescein is a synthetic organic compound that is slightly soluble in water and does not contain specific active groups, which greatly limits its ability to enter tumor cells

Method used

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  • Method for constructing multifunctional upconversion nano-platform

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Embodiment 1

[0036] A method for constructing an HSA-coupled multifunctional upconversion nano-platform, comprising the following steps:

[0037] A. Preparation of mercaptopropionic acid-modified GdF by hydrothermal method 3 :20%Yb 3+ ,1%Er 3+ Up-conversion fluorescent nanomaterials (for the preparation method, please refer to the Chinese invention patent application "A GdF3 Yb 3+ ,Er 3+ Preparation method of up-conversion fluorescent nanomaterials", patent number: 2017103774262) or water-soluble UCNPs prepared by other methods;

[0038] B. Weigh 2 mg of GdF modified with mercaptopropionic acid 3 :20%Yb 3+ ,1%Er 3+ Up-convert fluorescent nanomaterial powder, dissolve it in 2 mL MES buffer solution, and sonicate for 10 minutes;

[0039] C. Weigh 2 mg of EDC powder, dissolve it with the mixed solution obtained in step B, and shake it at room temperature for 15 minutes in the dark;

[0040] D. Weigh 1 mg of Sulfo-NHS powder, dissolve it with the mixed solution obtained in step C, and ...

Embodiment 2

[0047] A method for constructing an Ab-AFP coupled multifunctional upconversion nano-platform, comprising the following steps:

[0048] A. Preparation of mercaptopropionic acid-modified GdF by hydrothermal method 3 :20%Yb 3+ ,1%Er 3+ Up-conversion fluorescent nanomaterials (for the preparation method, please refer to the Chinese invention patent application "A GdF 3 Yb 3+ ,Er 3+ Preparation method of up-conversion fluorescent nanomaterials", patent number: 2017103774262) or water-soluble UCNPs prepared by other methods;

[0049] B. Weigh 2 mg of GdF modified with mercaptopropionic acid 3 :20%Yb 3+ ,1%Er 3+ Up-convert fluorescent nanomaterial powder, dissolve it in 2 mL MES buffer solution, and sonicate for 10 minutes;

[0050] C. Weigh 2 mg of EDC powder, dissolve it with the mixed solution obtained in step B, and shake it at room temperature for 15 minutes in the dark;

[0051] D. Weigh 1 mg of Sulfo-NHS powder, dissolve it with the mixed solution obtained in step C,...

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Abstract

The invention discloses a method for constructing a multifunctional upconversion nano-platform. The method includes loading antibodies or multifunctional human serum albumin on the surfaces of water-soluble upconversion nanoparticles through chemical bonding, and loading one or more fluoresceins on the surfaces of active groups such as the antibodies through hydrophobic interaction between proteins and fluorescein molecules so as to construct the multifunctional upconversion nano-platform. Compared with the prior art, the method has the advantages that a nano-composite prepared by the method is highly targeted, good in biocompatibility, simple in technological process, low in cost and easy to popularize and apply on a large scale, and the loaded fluorescein molecules are high and adjustable in concentration; the method has a promising application prospect in the field of biomedicine, tumor imaging and targeted delivering of anti-cancer drugs in particular.

Description

technical field [0001] The invention relates to the field of luminescent materials, in particular to a method for constructing a multifunctional up-conversion nanometer platform. Background technique [0002] According to data from 72 cancer registration points compiled by the National Cancer Center, there were approximately 4,292,000 new cancer cases and 2,814,000 cancer deaths in my country in 2015, accounting for about 22% of new cancer cases worldwide. 27%. The global cancer epidemiological statistics show that in recent years, the risk of cancer incidence and death in developing countries has a relatively higher trend. The key to improving the quality of life of patients and prolonging their survival period is the development and development of early cancer diagnosis and treatment technology. Nowadays, the early diagnosis and treatment of cancer in our country are facing the following problems: first, the early diagnosis methods used for tumor patients have poor sensit...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/574G01N33/577
CPCG01N33/533G01N33/57496G01N33/577
Inventor 王百齐李坤萌张利文张强高磊曹德庆王智宇王彤洪恩律高娜
Owner TIANJIN MEDICAL UNIV
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