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Preparation method of photoelectrochemical aptamer sensor for detecting prostate-specific antigen

A prostate-specific, aptamer sensor technology, applied in electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of high cost, poor stability, unfavorable storage and transportation, etc., to improve sensitivity, avoid false positive and false negative errors , the effect of high stability

Active Publication Date: 2019-10-25
UNIV OF JINAN
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AI Technical Summary

Problems solved by technology

Because antibodies need to be produced by cell lines, which have high costs, and their poor stability is not conducive to storage and transportation under harsh conditions, therefore, it is necessary to develop new molecular recognition tools

Method used

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Examples

Experimental program
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Embodiment 1

[0020] Embodiment 1: photoelectrochemical aptasensor is used for the detection of prostate-specific antigen

[0021] (1) Design the hydrophobic wax printing pattern of the paper chip with the computer software Adobe illustrator CS6, then use the wax printer to print the hydrophobic wax printing pattern on the chromatographic paper, and heat it in an oven at 150 °C for 30 s to completely melt the wax and form Hydrophilic functional areas of unprinted wax;

[0022] (2) Print the printed patterns of carbon working electrode, carbon counter electrode and Ag / AgCl reference electrode designed by computer software Adobe illustrator CS6 on the unprinted wax pro- water function area;

[0023] (3) Grow a platinum nanoparticle layer on the working region of the carbon working electrode obtained in step (2) using the in situ reduction method: 30 μL of freshly prepared chloroplatinic acid at a concentration of 300 mM and boron at a concentration of 30 mM Add the sodium hydride mixture dr...

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Abstract

The invention discloses a preparation method of a photoelectrochemical aptamer sensor for detecting a prostate-specific antigen. Lots of cadmium sulfide quantum dots and a signal transduction probe having one end marked with graphene quantum dots are loaded on a paper-based platinum / zinc oxide nanosheet and a co-sensitization effect is activated, so that a photocurrent signal is increased; when anaptamer of a specific recognition prostate-specific antigen with gold nanoparticles labeled and the signal transduction probe are hybridized to form three strings of helical molecular switches, the co-sensitization effect is suppressed and a signal quenching effect of the gold nanoparticle is generated, and the photocurrent signal decreases; and when the prostate-specific antigen identifies the aptamer, the conformation of the three strings of helical molecular switches changes, the co-sensitization effect is reactivated, the quenching effect is eliminated, and the photocurrent signal is enhanced. And the sensitive detection of the prostate specific antigen is realized based on the converted signal.

Description

technical field [0001] The invention relates to the fields of paper chip technology, tumor marker detection technology, photoelectrochemical analysis technology and nanomaterial synthesis technology, and more specifically relates to a preparation method of a photoelectrochemical aptamer sensor for highly sensitive detection of prostate specific antigen. Background technique [0002] Sensitive detection of tumor markers is of great significance for cancer diagnosis, and various analytical methods have been developed. The photoelectrochemical analysis method is widely used in the detection of tumor markers because the detection signal and the excitation source are two completely separated forms, which can effectively reduce the background signal and enhance the analytical sensitivity. However, photoelectrochemical analysis methods usually use a single signal mode, which is prone to false positive or false negative errors during the analysis process, so it is necessary to devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/308G01N27/3278
Inventor 于京华杨红梅李正林颜梅葛慎光
Owner UNIV OF JINAN
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