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Biosensor for detection of mercury ions based on aptamer and preparation method thereof

A biosensor and nucleic acid aptamer technology, applied in the field of biosensors, can solve the problems of high cost, low specificity and sensitivity, and achieve the effects of low detection limit, improved sensitivity and mild reaction conditions

Inactive Publication Date: 2015-07-08
UNIV OF JINAN
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Problems solved by technology

[0005] In order to solve the problems of relatively low specificity and sensitivity and high cost of the method for detecting mercury ions in the above prior art, the present invention provides a nucleic acid aptamer-based detection method with high specificity and sensitivity, low cost and fast detection speed. Mercury ion biosensor

Method used

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  • Biosensor for detection of mercury ions based on aptamer and preparation method thereof
  • Biosensor for detection of mercury ions based on aptamer and preparation method thereof
  • Biosensor for detection of mercury ions based on aptamer and preparation method thereof

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Embodiment 1

[0048] as attached image 3 As shown, the biosensor provided by the present invention includes a gold electrode 1; HAP2 layer 2 and HAP1 and Probe1 layer 3.

[0049] Concrete preparation method of the present invention is as follows:

[0050] a. The gold electrode is first polished in 0.3 and 0.05 μm alumina slurry until it becomes a mirror surface, and then rinsed repeatedly with PBS and secondary water;

[0051] b. Add 10 μL of HAP2 (1.0 μM) dropwise to the electrode surface and incubate at room temperature for 2 hours. Fix the sulfhydryl chains to the electrode surface through Au-S bonds;

[0052] So far, the modification process of the electrode has come to an end. The following describes the reaction in the homogeneous solution and the main steps in the homogeneous reaction:

[0053] a. Mix sterilized water, 1X buffer, HAP1 (1.0μM), Probe1 (1.0μM), exonuclease III (1μL) and 1μL of different concentrations of target substances (10pM, 50pM, 100pM, 500pM , 1000pM, 5000pM, ...

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Abstract

The invention provides a biosensor for detection of mercury ions based on aptamer. An electrode is modified by an HAP2 layer and an HAP1 and probe1 layer in order. The invention also provides a preparation method of the biosensor. The biosensor provided by the invention has stable performance and good electrode repeatability, thus being suitable for mercury ion detection in food safety and biosensor industrialization practical application.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a biosensor for detecting mercury ions based on nucleic acid aptamers, and also relates to a preparation method thereof. Background technique [0002] Mercury and its compounds have many kinds of harm to human health, and if they exist in natural water bodies, they will pose a threat to a wide range of people. It can accumulate in organisms and transfer to the human body through the food chain. The trace mercury accumulated in the human body cannot be excreted through its own metabolism, which will directly lead to heart, liver and thyroid diseases, nervous system disorders, chronic mercury poisoning, and even the formation of malignant tumors. [0003] dissolved Hg 2+ It often has high chemical activity and is the main form of mercury pollutants discharged into natural water bodies. Its compounds have high water solubility and are also the hub for the transformation of...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/26
Inventor 刘素宋晓蕾王玉黄加栋王虹智徐伟郭玉娜许颖邱婷婷崔洁
Owner UNIV OF JINAN
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