Biosensor for detection of mercury ions based on aptamer and preparation method thereof
A biosensor and nucleic acid aptamer technology, applied in the field of biosensors, can solve the problems of high cost, low specificity and sensitivity, and achieve the effects of low detection limit, improved sensitivity and mild reaction conditions
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[0048] as attached image 3 As shown, the biosensor provided by the present invention includes a gold electrode 1; HAP2 layer 2 and HAP1 and Probe1 layer 3.
[0049] Concrete preparation method of the present invention is as follows:
[0050] a. The gold electrode is first polished in 0.3 and 0.05 μm alumina slurry until it becomes a mirror surface, and then rinsed repeatedly with PBS and secondary water;
[0051] b. Add 10 μL of HAP2 (1.0 μM) dropwise to the electrode surface and incubate at room temperature for 2 hours. Fix the sulfhydryl chains to the electrode surface through Au-S bonds;
[0052] So far, the modification process of the electrode has come to an end. The following describes the reaction in the homogeneous solution and the main steps in the homogeneous reaction:
[0053] a. Mix sterilized water, 1X buffer, HAP1 (1.0μM), Probe1 (1.0μM), exonuclease III (1μL) and 1μL of different concentrations of target substances (10pM, 50pM, 100pM, 500pM , 1000pM, 5000pM, ...
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