The present invention discloses primers, probes, a detection
system and a kit for one time detection of
lung cancer multiple genes, wherein the primers, the probes, and the distribution way for detecting 25 EGFR
gene mutations, 7
KRAS gene mutations, 6 BRAF
gene mutations, 9 NRAS gene mutations, 5 HER2 gene mutations, 2 PIK3CA gene mutations, 5 fusion genes of ALK5, 13 fusion genes of ROS1, and 6 fusion genes of RET are provided. According to the present invention, the detection kit adopts the 12 linking PCR reaction strip design, each 12 linking PCR strip detects multiple genes of a sample, and the corresponding fusion detection reagents and the internal control reagents are filled in the pipes 1-4 of the 12 linking PCR strip; and with the primers, the probes, the detection
system and the kit, the one-time detection of the 24 fusions and the 54 mutations of the
lung cancer can be achieved, such that the detection time is substantially shortened, the sensitivity is high, the specificity is strong, the operation is simple and rapid, and the reference for selection of
tumor targeting drug therapy on
lung cancer patients can be provided for clinician.