Test method of KRAS gene mutation for screening and assessing therapeutic effects of molecular targeted agents

A technology of molecular targeted drugs and detection methods, applied in biochemical equipment and methods, microbial determination/inspection, analytical materials, etc., can solve problems such as poor curative effect, and achieve stable extraction quality, high purity, and sample volume. less effect

Inactive Publication Date: 2010-02-24
上海中优医药高科技股份有限公司
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Problems solved by technology

Numerous studies have shown that for patients with tumors with KRAS mutations, the efficacy of these gene-targeted drugs is not good, mainly because KRAS is a downstream protein of the EGFR signaling pathway, and the continuous activation of mutant KRAS is related to whether EGFR activity is inhibited

Method used

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  • Test method of KRAS gene mutation for screening and assessing therapeutic effects of molecular targeted agents
  • Test method of KRAS gene mutation for screening and assessing therapeutic effects of molecular targeted agents
  • Test method of KRAS gene mutation for screening and assessing therapeutic effects of molecular targeted agents

Examples

Experimental program
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Embodiment

[0058] A method for detecting the curative effect of an epidermal growth factor receptor tyrosine kinase inhibitor, the specific steps are:

[0059] Step 1. Collecting serum or tumor tissue samples from the subject;

[0060] Step 2. Extraction method of genomic DNA:

[0061] Use the silica gel adsorption method to extract the DNA of the test subject in step 1. The extraction time is 2-3 hours. Use the electrophoresis gel imaging method to detect the DNA concentration and purity. Clear white strips can be seen with the naked eye to judge that the obtained DNA can enter Next step detection;

[0062] Step 3. Genotyping:

[0063] Put each test sample into a reaction well, use PCR technology to amplify, gel electrophoresis technology to detect purity, and sequencing technology to determine the genotype of these sites:

[0064] Step 3-1, reaction system configuration:

[0065] Add PCR standard reaction system in each reaction well, the primer concentration of standard reaction s...

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Abstract

The invention relates to a test method of KRAS gene mutation for screening and assessing therapeutic effects of molecular targeted agents, which is characterized by the following specific steps: acquiring serum or tumor tissue samples of testees, extracting genomic DNA, testing a No.2 exon of the KRAS gene, designing corresponding site-specific primers and adopting experimental schemes, reagents and report generation systems for sequence determination and assessing the effectiveness of EGFR tyrosine kinase inhibitors used for treating cancer individuals by simultaneously testing and analyzingwhether mutation exists on the No.2 exon of the individual KRAS gene. The method has the advantage of being used for application of the EGFR tyrosine kinase inhibitors used for treating cancer individuals.

Description

technical field [0001] The invention relates to a detection method for KRAS gene mutation screening and evaluation of the curative effect of molecular targeted drugs, and evaluates the epidermal growth factor receptor tyrosine for treating cancer individuals by detecting and analyzing whether there is a mutation on the No. 2 exon of the individual KRAS gene The invention relates to the effectiveness of kinase inhibitor drugs, belonging to the technical field of drug detection. Background technique [0002] Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI) such as Avastin (AVASTIN), Tarceva (Erlotinib), gefitinib (irressa), cetuximone (Erbitux), etc. can inhibit Epidermal growth factor receptor tyrosine kinase, which is necessary for tumorigenesis and development, blocks the signal transduction of tumor cells, thereby inhibiting tumor cell proliferation, invasion, metastasis, angiogenesis and promoting tumor cell apoptosis. The KRAS gene is involved in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N27/447
Inventor 王校傅咏南
Owner 上海中优医药高科技股份有限公司
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