Colorectal cancer auxiliary diagnosis kit for fecal nucleic acid detection and use method thereof

A technology for colorectal cancer and detection reagents, which is applied in the fields of biotechnology and medicine, can solve the problems of low sensitivity and specificity, and achieve high sensitivity and specificity, and good effect of medication guidance and evaluation

Active Publication Date: 2020-03-24
GENETALKS BIO TECH CHANGSHA CO LTD
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For this reason, the present invention proposes a colorectal cancer auxiliary diagnostic kit for stool nucleic acid detection, which can...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Colorectal cancer auxiliary diagnosis kit for fecal nucleic acid detection and use method thereof
  • Colorectal cancer auxiliary diagnosis kit for fecal nucleic acid detection and use method thereof
  • Colorectal cancer auxiliary diagnosis kit for fecal nucleic acid detection and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] In order to describe the technical content, achieved objectives and effects of the present invention in detail, the following descriptions will be made in conjunction with the embodiments.

[0034] Implementation 1: Sampling

[0035] Use 15mL of the feces sample preservation solution (public number: CN107980763A, the feces preservation reagent prepared in the example) that our company has independent patent rights to collect 5-8g of feces samples, and then use the feces sample preservation solution for sample processing.

[0036] Implementation 2: Extraction

[0037] The magnetic bead method extraction kit (nucleic acid extraction or purification reagent, model G1201) developed by our company is used for extraction. The specific steps are as follows:

[0038] 1. Fully shake and mix the received sample (about 20mL) to make the sample solution in a homogenized state, centrifuge at 3000g for 15min, transfer 10mL supernatant to another 50mL centrifuge tube, and save the re...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a colorectal cancer auxiliary diagnosis kit for fecal nucleic acid detection and a use method of the colorectal cancer auxiliary diagnosis kit. The kit comprises a KRAS gene mutation detection reagent, a fusobacterium nucleatum detection reagent, a DNA sulfite conversion reagent and a Septin9, SDC2, HOXA11 and NDRG4 gene methylation detection reagent. The use method comprises the following steps: collecting an excrement sample, and then completing DNA purification, KRAS gene mutation detection, fusobacterium nucleatum abundance detection and Septin9, SDC2, HOXA11 and NDRG4 gene methylation detection of the sample by using reagents in the kit; and calculating the obtained data through a logistic regression model, and performing result evaluation and analysis. The colorectal cancer detection sensitivity reaches 89% or above, the specificity reaches 94% or so, high-sensitivity and high-specificity colorectal cancer detection can be achieved, and doctors can be helped to guide follow-up treatment medication of patients.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to a colorectal cancer auxiliary diagnosis kit for detection of stool nucleic acid and a use method thereof. Background technique [0002] Colorectal cancer is one of the most common malignant tumors in clinical practice. The number of new cases of colorectal cancer in the world is about 1.2 million every year, and the annual death toll exceeds 600,000. The incidence rate ranks third and the case fatality rate ranks fourth. In recent years, the incidence of colorectal cancer has shown an obvious upward trend. According to the latest "China Cancer Registration Annual Report" issued by the National Cancer Registry Center, the incidence and death of colorectal cancer and other digestive tract cancers are increasing year by year nationwide, the number of new cases is about 400,000 per year, and the number of deaths is about 195,000 people / year. It takes a relatively long tim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6886C12Q1/689C12Q1/04C12N15/11
CPCC12Q1/6886C12Q1/689C12Q2600/154C12Q2600/156C12Q2600/166
Inventor 王益民周巧高堂杰戴应何庆杨婷曹叶
Owner GENETALKS BIO TECH CHANGSHA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap