Colorectal cancer auxiliary diagnosis kit for fecal nucleic acid detection and use method thereof

Abstract

The invention discloses a colorectal cancer auxiliary diagnosis kit for fecal nucleic acid detection and a use method of the colorectal cancer auxiliary diagnosis kit. The kit comprises a KRAS gene mutation detection reagent, a fusobacterium nucleatum detection reagent, a DNA sulfite conversion reagent and a Septin9, SDC2, HOXA11 and NDRG4 gene methylation detection reagent. The use method comprises the following steps: collecting an excrement sample, and then completing DNA purification, KRAS gene mutation detection, fusobacterium nucleatum abundance detection and Septin9, SDC2, HOXA11 and NDRG4 gene methylation detection of the sample by using reagents in the kit; and calculating the obtained data through a logistic regression model, and performing result evaluation and analysis. The colorectal cancer detection sensitivity reaches 89% or above, the specificity reaches 94% or so, high-sensitivity and high-specificity colorectal cancer detection can be achieved, and doctors can be helped to guide follow-up treatment medication of patients.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to a colorectal cancer auxiliary diagnosis kit for detection of stool nucleic acid and a use method thereof. Background technique [0002] Colorectal cancer is one of the most common malignant tumors in clinical practice. The number of new cases of colorectal cancer in the world is about 1.2 million every year, and the annual death toll exceeds 600,000. The incidence rate ranks third and the case fatality rate ranks fourth. In recent years, the incidence of colorectal cancer has shown an obvious upward trend. According to the latest "China Cancer Registration Annual Report" issued by the National Cancer Registry Center, the incidence and death of colorectal cancer and other digestive tract cancers are increasing year by year nationwide, the number of new cases is about 400,000 per year, and the number of deaths is about 195,000 people / year. It takes a relatively long tim...

AI Technical Summary

Problems solved by technology

For this reason, the present invention proposes a colorectal cancer auxiliary diagnostic kit for stool nucleic acid detection, which can solve the shortcomings of the prior art in the detection of colorectal cancer lesions by stool genes, such as low sensitivity and specificity

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