Cleaning agent, and preparation method and application thereof
A purification agent, the technology of Bacillus natto, applied in the field of biochemistry, can solve the problems such as unsatisfactory purification ability, achieve good water purification effect, simple production process, and good water purification effect
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[0051] The present invention will be described in further detail and completely below in conjunction with the embodiments.
[0052] Required instruments and reagents:
[0053] (1) Instrument:
[0054] Ultra-clean workbench, high-pressure steam sterilizer, constant temperature shaker, fermenter, freeze dryer, biochemical incubator, drying oven, pulverizer.
[0055] (2) Reagents:
[0056] LB liquid medium was prepared from 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl and 950 m of water, prepared according to the guidance of "Molecular Cloning Experiment Guide" (written by J. Sambrook D.W. Russell).
[0057] The strain of Bacillus natto was isolated and identified by the National Aquatic Animal Pathogen Bank of Shanghai Ocean University, numbered JS-20121101.
[0058] Bacillus natto strain fermentation medium, the formula is 37% soluble starch, 30% soybean powder, 20% fish meal, 10% molasses, 3% sodium chloride, prepared according to the guidance of "Microbiology Tutor...
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