Cleaning agent, and preparation method and application thereof

A purification agent, the technology of Bacillus natto, applied in the field of biochemistry, can solve the problems such as unsatisfactory purification ability, achieve good water purification effect, simple production process, and good water purification effect

Inactive Publication Date: 2017-01-18
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of Bacillus microorganisms alone in the water body can make the eutrophication substances flowing into the water body absorbed by these beneficial microorganisms, and they digest these "pollutants" through their own functions, so that the pollution can be controlled, but its purification ability It is not ideal enough, so it is very important for the existing water body purification field to study a kind of very good water purification agent for the ability to deal with eutrophic substances; better, if the water body purification agent is purifying the water body, it will It can improve the disease resistance of fish, crab, shrimp and shellfish in the water body, and the market prospect will be broader

Method used

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  • Cleaning agent, and preparation method and application thereof
  • Cleaning agent, and preparation method and application thereof
  • Cleaning agent, and preparation method and application thereof

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Embodiment Construction

[0051] The present invention will be described in further detail and completely below in conjunction with the embodiments.

[0052] Required instruments and reagents:

[0053] (1) Instrument:

[0054] Ultra-clean workbench, high-pressure steam sterilizer, constant temperature shaker, fermenter, freeze dryer, biochemical incubator, drying oven, pulverizer.

[0055] (2) Reagents:

[0056] LB liquid medium was prepared from 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl and 950 m of water, prepared according to the guidance of "Molecular Cloning Experiment Guide" (written by J. Sambrook D.W. Russell).

[0057] The strain of Bacillus natto was isolated and identified by the National Aquatic Animal Pathogen Bank of Shanghai Ocean University, numbered JS-20121101.

[0058] Bacillus natto strain fermentation medium, the formula is 37% soluble starch, 30% soybean powder, 20% fish meal, 10% molasses, 3% sodium chloride, prepared according to the guidance of "Microbiology Tutor...

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Abstract

The invention discloses a cleaning agent. The cleaning agent comprises Bacillus natto, Paracoccus denitrificans, Streptococcus faecalis and a nutrient buffer system, wherein the concentration of live bacteria of Bacillus natto is greater than 20 billion per g. The cleaning agent provided by the invention has the beneficial effects that (1) when used in aquaculture, the cleaning agent can efficiently remove ammonia nitrogen and nitrite in water, purify water, eliminate algae, increase transparency, improve water color highly efficiently eliminate contamination, completely decompose residual feeds, manure and the like, enhance the ingestion capacity of aquatic livestock like fish, shrimps, crabs and mussels, decrease stress, substantially promote growth of aquaculture animals and improve the survival rate of aquaculture animals; and (2) when applied to rivers and landscape water bodies, the cleaning agent can efficiently remove ammonia nitrogen and nitrite in water, purify water, eliminate algae, increase transparency and improve water color.

Description

technical field [0001] The invention belongs to the field of biochemistry and relates to a purifying agent, in particular to a purifying agent and its preparation method and application. Background technique [0002] Water pollution is an important aspect of environmental pollution. Nearly half of the country's seven major water systems are seriously polluted, and most rivers near cities are polluted to varying degrees. With the rapid development of chicken farms, pig farms, and cattle farms, the treatment of waste has not received enough attention, resulting in a large amount of livestock manure and other direct discharges into water bodies, which intensifies the eutrophication of lakes and coastal waters in my country . When there are too many nutrients in the water body of the lake, algae will grow, roots or plankton will grow in large numbers, which will cause harm to the normal function of the lake water body. For example: lead to changes in the odor and color of drink...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/46C12R1/01
Inventor 胡鲲
Owner SHANGHAI OCEAN UNIV
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