A kind of microbial organic fertilizer for preventing and treating bacterial wilt and its preparation and application method
A technology for microbial organic fertilizer and bacterial wilt disease, applied in the field of biological fertilizers, can solve problems such as poor effect and unstable effect, and achieve the effect of obvious effect and yield improvement.
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[0019] (1) Isolation and identification of strains
[0020] Isolation of Aspergillus niger, Trichoderma viride, and Bacillus licheniformis: These three bacteria were separated from garden waste and straw piles, and separated by streaking on a plate with garden waste as the only carbon source of the medium;
[0021] Pichia burtonii was purchased from Guangdong Institute of Microbiology.
[0022] Isolation of Bacillus subtilis: The bacterium was sampled from the rhizosphere soil of crops where bacterial wilt occurs, and was isolated by dilution plate method and antibacterial method.
[0023] Screening and isolation of Bacillus glialis and Bacillus pumilus: samples were taken from the rhizosphere soil of crops, and screening and isolation were carried out by the growth promotion contrast method and the dilution plate method.
[0024] Identification of Aspergillus niger, Trichoderma viridans, Bacillus licheniformis, Bacillus subtilis, Bacillus coli, and Bacillus pumilus: traditio...
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