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Rhodopseudomonas strain and application thereof in inhibiting Pseudoperonospora cubensis Rostov and promoting cucumber growth

A technology of rhodopseudomonas and strains, applied in plant growth regulators, microbe-based methods, chemicals for biological control, etc., can solve the problems of no photosynthetic bacteria, and achieve obvious control of cucumber downy mildew effect, low production cost, and the effect of promoting cucumber growth

Active Publication Date: 2014-02-26
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some good microbial resources have been excavated in the control of cucumber downy mildew. However, there is no report on the application of photosynthetic bacteria to cucumber downy mildew.

Method used

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  • Rhodopseudomonas strain and application thereof in inhibiting Pseudoperonospora cubensis Rostov and promoting cucumber growth
  • Rhodopseudomonas strain and application thereof in inhibiting Pseudoperonospora cubensis Rostov and promoting cucumber growth
  • Rhodopseudomonas strain and application thereof in inhibiting Pseudoperonospora cubensis Rostov and promoting cucumber growth

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Embodiment

[0033] A facultative anaerobic rhodopseudomonas strain of the present invention, which is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M2012366. The Rhodopseudomonas CCTCC M2012366 was named as Rhodopseudomonas sp.S-1.

[0034] After our detection, the Rhodopseudomonas CCTCC M2012366 of this embodiment has the following main characteristics:

[0035] G-, the individual shape is rod-shaped, V-P reaction is positive, methyl red reaction is positive, starch cannot be utilized, H 2 S reaction is positive, gelatin liquefaction is positive, indole reaction is positive, citrate test is negative, urease test is negative, catalase test is positive, can grow in 3% NaCl, the optimum growth temperature is 30℃~35℃. The growth pH is 6.5-7.5, the characteristic absorption peaks of the bacteria are 375nm, 805nm and 865nm, the measured 16S rDNA sequence length is 1389bp, and the accession number in Genbank is KC491187.

[0036] The above...

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Abstract

The invention discloses a facultative anaerobic Rhodopseudomonas strain which is collected in China Center for Type Culture Collection, and the collection number is CCTCC NO:M2012366. The Rhodopseudomonas strain can inhibit Pseudoperonospora cubensis Rostov and promote cucumber growth. When in use, a photosynthetic bacteria liquid can be directly sprayed, or prepared into a fermentation liquid and sprayed. The preparation method of the photosynthetic bacteria liquid and photosynthetic bacteria fermentation liquid comprises the following steps: activating the Rhodopseudomonas strain; inoculating the activated and cultured single colony into a serum bottle, and carrying out seed culture; carrying out production culture to obtain the photosynthetic bacteria liquid; and centrifugating the photosynthetic bacteria liquid, collecting the supernate, and filtering through a biofilter to obtain the photosynthetic bacteria fermentation liquid. The method has the advantages of low production cost, favorable antagonistic effect, high efficiency, no toxicity, environmental protection and the like.

Description

technical field [0001] The invention relates to a photosynthetic bacterium and its application, in particular to a rhodopseudomonas strain and its application in inhibiting cucumber downy mildew and promoting cucumber growth. Background technique [0002] Cucumber downy mildew is one of the most important diseases affecting cucumber planting. During the entire growth period of cucumber, if the disease conditions are suitable, whether it is cultivated in the open air or in a protected area, its penetration and spread are extremely fast, and it can affect the growth of cucumbers. There was a severe loss of production. At present, the most important and effective measure to control cucumber downy mildew is to use fungicides. However, due to the selection pressure of chemical agents, the unreasonable use of a single agent or a single type of fungicide can easily lead to a rapid increase in the resistance of pathogenic bacteria, and further This leads to an increase in the use o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00A01P21/00C12R1/01
Inventor 刘勇张德咏彭静张松柏高阳张卓程菊娥谭新球成飞雪朱春晖杨春晓
Owner HUNAN PLANT PROTECTION INST
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