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Biological evaluation method capable of quantitatively characterizing polycyclic aromatic hydrocarbon adsorption capacity of modified biochar in water body

A polycyclic aromatic hydrocarbon and quantitative characterization technology is applied in the field of biological evaluation for quantitatively characterizing the ability of modified biochar to adsorb polycyclic aromatic hydrocarbons in water, which can solve the problem that biological evaluation indicators have not yet appeared.

Pending Publication Date: 2021-07-06
TIANJIN VOCATIONAL INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in addition to these physical and chemical indicators, biological evaluation indicators that can quantitatively characterize and compare the performance of different modified biochars to adsorb PAHs in water have not yet appeared.

Method used

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  • Biological evaluation method capable of quantitatively characterizing polycyclic aromatic hydrocarbon adsorption capacity of modified biochar in water body
  • Biological evaluation method capable of quantitatively characterizing polycyclic aromatic hydrocarbon adsorption capacity of modified biochar in water body
  • Biological evaluation method capable of quantitatively characterizing polycyclic aromatic hydrocarbon adsorption capacity of modified biochar in water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: The lowest total lethal concentration LC 100 Determination test

[0046] In order to measure the lowest total lethal concentration LC of four kinds of PAHs mixtures in the 48h acute toxicity test of Daphnia magna 100 , respectively pipette 0.375mL, 0.563mL, 0.844mL, 1.266mL, 1.898mL of the aforementioned four kinds of PAHs mixture saturation test solutions to a 250mL volumetric flask, add ISO standard dilution water to constant volume, and obtain concentrations of 0.15%, 0.23%, 0.34% , 0.51%, and 0.76% five test liquids, with ISO standard dilution water as blank control.

[0047] The test adopts the static method, and the control group and the test group are set for 4 repetitions, with 5 Daphnia magna and 50 mL of the test solution for each repetition. The test exposure time was 48h. The daphnia to be tested were randomly selected, and the test solution was added after reaching the test temperature, and the adding process was completed within 30 minutes. ...

Embodiment 2

[0057] Example 2: Acute Toxicity Test of Daphnia magna under Charcoal Y Adsorption of PAHs

[0058] Pipette 1.266mL of the saturated test solution of the four kinds of PAHs mixtures into six 250mL volumetric flasks, and obtain the PAHs solution at the lowest total lethal concentration of 0.51mg / L after constant volume with ISO standard dilution water. Transfer the solution to five 250mL Erlenmeyer flasks with stoppers, then weigh 0.05g, 0.10g, 0.20g, 0.40g, and 0.80g of carbon Y into the above-mentioned Erlenmeyer flasks, and mix them in a shaker at 20°C at 150r / min Adsorbed for 24 hours, the filtrate filtered through a 0.45 μm pore size filter membrane was used as the test solution, and 200 mL of PAHs solution was measured from the sixth volumetric flask as a blank control.

[0059] The test adopts the static method, and the control group and the test group are set for 4 repetitions, with 5 Daphnia magna and 50 mL of the test solution for each repetition. The test exposure t...

Embodiment 3

[0068] Example 3: Acute Toxicity Test of Daphnia magna under Charcoal G Adsorption of PAHs

[0069] Pipette 1.266mL of the saturated test solution of the four PAHs mixtures into six 250mL volumetric flasks, and dilute to volume with ISO standard dilution water to obtain the PAHs solution at the lowest total lethal concentration of 0.51mg / L. Transfer the 200mL solutions in five volumetric flasks to five 250mL corked Erlenmeyer flasks respectively, then weigh 0.025g, 0.05g, 0.10g, 0.20g, and 0.40g of carbon G into the above-mentioned Erlenmeyer flasks, and Mix and adsorb in a shaker at 20°C at 150r / min for 24 hours, and the filtrate filtered through a 0.45 μm pore size filter membrane was used as the test solution, and 200 mL of PAHs solution was taken from the sixth volumetric flask as a blank control.

[0070] The test adopts the static method, and the control group and the test group are set for 4 repetitions, with 5 Daphnia magna and 50 mL of the test solution for each repet...

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Abstract

The invention discloses a biological evaluation method capable of quantitatively characterizing the capacity of modified charcoal for adsorbing polycyclic aromatic hydrocarbon in a water body. The method comprises the following steps: firstly, establishing a linear regression equation reflecting a dose-effect relationship between the dosage of to-be-detected modified charcoal and the daphnia magna death rate; then selecting the dosage of the modified charcoal to be detected according to the death rate of ideal daphnia magna in the water body; or under the same dosage, comparing and evaluating the death rate of daphnia magna according to the death rate of daphnia magna. According to the method, the death rate of daphnia magna can be used as a biomarker for quantitatively characterizing the dosage of single carbon, and also can be used as a biological evaluation index for quantitatively characterizing and comparing the performance of repairing PAHs polluted water bodies by different carbons.

Description

technical field [0001] The invention belongs to the technical field of water environment protection, and specifically relates to a biological evaluation method capable of quantitatively characterizing the ability of modified biochar to absorb polycyclic aromatic hydrocarbons in water bodies. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants (POPs) that are ubiquitous in the environment. The most important pollution characteristic of PAHs is the universality of pollution, that is, PAHs are widely distributed in various environmental media such as water, soil, sediment, and atmosphere. Among them, water, suspended solids and sediments are the main gathering places of polycyclic aromatic hydrocarbons. [0003] In recent years, many modified biochars prepared by various modification technologies (physical, chemical and biological) have been widely used in the adsorption and removal of PAHs in water bodies, and the performanc...

Claims

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Application Information

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IPC IPC(8): G01N33/18
CPCG01N33/186
Inventor 闫东科许凤霞张发荣李璐吕平梁丽娟李陇梅宫艳超栗煜李冬李德超童文辉顾佳萌范思麒孟鑫张嘉铖李忠元周一伟
Owner TIANJIN VOCATIONAL INST
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