161 results about "Dose-effect relationship" patented technology

The invention discloses an effect target device for shock wave pressure measurement of a target of a moving explosive field. The effect target device is characterized by comprising a base, an intermediate body, a response diaphragm, a cover plate, a sealing hole, a pressure leading hole, compression bolt holes, compression bolts, an auxiliary hole, and an auxiliary plate. When an external shock wave is applied to the effect target device, the wave is applied to five working areas of the response diaphragm through the pressure leading hole of the cover plate to cause plastic deformation and recession into the sealing hole below; a maximum deflection value is measured; and according to a dose-effect relationship between diaphragm deformation and an impact shock effect, an impact wave pressure peak value is obtained. According to the invention, the effect target device has the following advantages: the effect target device with the light weight is easy to arrange; the sealing performanceis good; the interference of the shock wave diffraction is eliminated; the effective range is wide; the pressure range capable of damaging the target is covered basically; a measurement problem of a large shock wave pressure changing range of the target of the moving explosive field is solved; the precision and reliability of the target pressure measurement in the moving explosive field are improved; and the need of the shock wave pressure measurement of the target of the moving explosive field is met.

The invention discloses a water comprehensive toxicity rapid detection method based on algae chlorophyll fluorescene. According to the method, toxic and sensitive unicellular scenedesmus obliquus is selected as a testing organism, an experimental group and a blank control group of a to-be-tested water sample are subjected to adaptive cultivation for 24 hours, fast-phase chlorophyll fluorescene of two groups of algae samples is measured, fluorescence parameters in the energy transfer process of main photosynthesis are analyzed and obtained, an inhibition ratio of to-be-tested water of the experimental group on the photosynthesis of algae is calculated, and the comprehensive toxicity intensity of the to-be-tested water is calculated according to the dose-effect relationship between the substance toxicity intensity and the inhibition ratio of photosynthesis. The method has the characteristics of sensitive toxicity response, simple and fast test and the like and is a new method for rapid evaluation of ecological risk and emergency monitoring of sudden pollution accidents.

The invention discloses application of curcumin to preparation of medicaments for preventing and/or treating autosomal dominant polycystic kidney disease. A madin-darby canine kidney (MDCK) vesicle model is used for screening to obtain the curcumin which inhibits formation and growth of vesicles. An experimental result shows that: the curcumin has an obvious inhibiting effect on the formation and growth of MDCK vesicles and the effect of the curcumin is in dose response relationship; the curcumin has no cytotoxicity to MDCK cells, so that the vesicle inhibiting effect of the curcumin is independent of the cytotoxicity; the curcumin does not obviously induce MDCK cell apoptosis, so that the vesicle inhibiting effect of the curcumin is independent of cell apoptosis promotion of the curcumin; the curcumin can promote the MDCK cells or vesicles to form tubular structures; and the effect is in dose response relationship; and the curcumin has an inhibiting effect on the growth of the embryonic kidney vesicles. The curcumin is expected to be developed into a specific medicament for preventing and/or treating autosomal dominant polycystic kidney disease.

The invention discloses a method for measuring superoxide anion radicals in tape grass leaves, belonging to the measuring field of the measuring of superoxide anion radicals. The method comprises the following steps of: firstly, collecting and cleaning the tip parts of fully exposed small and tender tape grass leaves; carrying out Tiron captureing on the Tiron of radicals in a closed operation box in a closed nitrogen environment; and finally measuring by using an EPR (Electron Paramagnetic Resonance) technology. The invention has the advantages of simpler operation and easy grasping, and can measure the superoxide anion radicals of the tape grass leaves in a very short time;, find that and the measured radicals are found to be the superoxide anion radicals according to the superfine structure constant and, the spectrogram shape analysis of an EPR spectrogram and the combination of the remarkable decreases of the radical strength of the leaves pretreated by SOD (Super Oxide Dismutase), and therefore the invention is the most direct, accurate and effective method for measuring the superoxide anion radicals. Superoxide anions are sensitive to pollutants and have a favorable dosage effect relation. The invention is also suitable for other aquatic plant and terrestrial plant species and can be suitable for measuring the radicals of the plant root tissue.

The invention belongs to the technical field of medicament and specifically relates to use of a deuteroporphyrin derivative. The invention provides use of deuteroporphyrin derivative in preparing medicament for treating squamous carcinoma or adenocarcinoma. The treatment with the deuteroporphyrin derivative or composition and medicament thereof to squamous carcinoma or adenocarcinoma has obvious growth-inhibiting effect and good dose effect relationship. A group with high dosage has strong and stable effect and stronger inhibiting effect than the positive control medicament, hemoporphyrin. The deuteroporphyrin derivative has no growth-inhibiting effect on tumour cells under a condition of non-laser radiation, no damaging effect on normal histiocytes with light or without light and little side effect.

The invention belongs to the field of researches on an electromagnetic biological effect and particularly relates to a living animal electromagnetic irradiation system and an application method thereof. The living animal electromagnetic irradiation system consists of an electromagnetic irradiation control system, a microwave device, a three-dimensional object carrying platform and a microwave anechoic chamber, wherein the electromagnetic irradiation control system at least comprises a hardware setting module, an electromagnetic irradiation parameter setting and output module, a microwave emission control module and an irradiation method and irradiation dose display module. All modules are mutually connected. The living animal electromagnetic irradiation system and the application method thereof have the advantages that multiple irradiation parameters which influence irradiation dose in animal bodies can be set through the electromagnetic irradiation control system, the placing posture of the living animals relative to the emitting direction of microwaves can be intuitively given out and can be adjusted through the three-dimensional object carrying platform, the irradiation dose of up to eleven important organs can be given out after the irradiation parameters are set, data is provided for establishing an accurate dose-effect relationship between the irradiation dose and the animal effect and the goal of remotely controlling the microwave device to emit microwaves is realized.

The invention discloses a connection effect target device suitable for measurement of target shock wave pressure of a movable explosion field. The effect target device is mainly characterized by beingcomposed of a base, a response membrane, a pressing ring, a buffering pad, a bottom cover and pressing bolts; external shock waves act on the effect target device and three working areas, with different pressure measurement ranges, on the response membrane after passing through pressure guiding holes, the maximal deflection value of the response membrane is measured after testing, and the pressure peak of the shock waves is obtained according to a dose-effect relationship of deformation of the membrane and the shock waves. The device has the significant advantages that the mass and size of aneffect target are greatly reduced, and movement, assembly and disassembly of the effect target in the movable explosion field are facilitated; circular structures are adopted for all parts of the effect target device, and the processing period can be shortened; on the condition that the size and mass of the effect target are reduced, the wide effective measuring range is guaranteed, a shock wavepressure range within which the target can be damaged is basically covered, and the requirement of the target of the movable explosion field for the measurement of shock wave pressure is met.

The invention discloses a method for testing toxicity of environmental estrogen on whitebait embryonic development. The method of the invention utilizes the sensitivity of embryo to the simulation of environmental pollutant or chemical matter to perform a test under semi-static state on whitebait embryo which is exposed in standard diluted aqueous solution of subject having a series of concentrations. The test lasts about 30 days, begins as exposing a live embryo at the blastula stage in the aqueous solution of subject, and ends with hatching all embryos in a control group and an exposed group. The toxicity endpoint contains death, monstrosity and delayed hatching. The method comprises the steps of confirming the toxicity of the subject on the whitebait embryonic development by observing the toxicity endpoint of the exposed group and comparing with the control group; researching on the influence of two natural estrogens E2 and EE2 on the whitebait embryo-yolk sac stage so as to confirm the toxic dose-effect relationship of the natural estrogens to the whitebait embryonic development, and evaluating the potential risk of this type of fish exposed under the estrogen with environmental concentration.

The invention relates to an oroxylum indicum general flavone extraction and purification method. Ethyl alcohol backflow and macroporous resin adsorption are used for separating, extracting and purifying oroxylum indicum general flavone, non-flavonoid impurities are removed as many as possible, and finally enrichment is carried out to obtain the high-purity general flavone. The oroxylum indicum general flavone comprises five chemical components including semen oroxyli glycoside B, semen oroxyli glycoside A, chrysin-7-O-beta-D-glucuronide, baicalein and chrysin. The invention further provides application of the oroxylum indicum general flavone to prepating medicine for treating liver cancers. In-vitro pharmacological tests show that the general flavone has the obvious restraining effect on proliferation of liver cancer cells and trends to have the obvious dose-effect relationship, and meanwhile the pharmacological effect is obviously improved along with increase of the purity of the general flavone; in addition, oroxylum indicum general flavone matter analysis studying is carried out, the material basis of oroxylum indicum for playing a role in anti-cancer pharmacological effect is defined, and the material basis and the experiment basis are laid for developing clinical application of oroxylum indicum general flavone enrichment matter, anti-cancer new medicine researching and development and industrial production.

The invention relates to a bagged tea containing plant flowers, stigma and leaves and a preparation method thereof. The bagged tea is produced by pretreating corn stigma, Cassia occidentalis, chrysanthemum flower, hawthorn and pagodatree flowers and leaves, boiling in water; filtering, collecting supernatant, concentrating, extracting with ethanol, removing ethanol, uniformly spraying the resulting ethanol extract to lotus leaf coarse powder, adding sugar powder 6 times the weight of the drug powder and dextrin double the weight of the drug powder, and stirring thoroughly. The bagged tea has good palatability, convenient use, high therapeutic effect, and hypolipidemic and anti-obesity effects. It is proven that the blood fat level is significantly reduced after taking the bagged tea continuously for 30-40 days, with a dose-effect relationship; and the body weight is remarkably reduced after taking the bagged tea continuously for more than 60 days.

The invention discloses an enzyme process extracted Radix angelicae anomalae polysaccharide, and a preparation method and an application thereof. The Radix angelicae anomalae polysaccharide has alpha-configuration and beta-configuration polysaccharide linking modes, the apparent molecular weight of the Radix angelicae anomalae polysaccharide is 3.93 kDa. The Radix angelicae anomalae polysaccharide is obtained through enzyme process extraction of a Radix angelicae anomalae medicinal material, alcohol precipitation and impurity removal, protein and salt removal, and DEAE-agarose gel FF column and agarose gel 6FF gel column chromatography separation. The Radix angelicae anomalae polysaccharide obtained in the invention has good whitening activity and has an inhibitory effect on the tyrosinase activity, and the tyrosinase inhibition rate gradually increases with the increase of concentration, and the dose-effect relationship is obvious. The method for extracting the Radix angelicae anomalae polysaccharide has the advantages of small dosage of an enzyme preparation, simple process and low production cost.

The invention discloses application of Anerning granules in preparing a medicament for treating viral pneumonia. The granules have a remarkable curative effect on viral pneumonia caused by respiratory syncytial virus and influenza virus, can be used for remarkably reducing the lung index, and is in dose-effect relationship. The Anerning granules can be used for protecting experimental animal death caused by respiratory syncytial virus (RSV), and has a remarkable inhibition effect on spleen index and thymus index reduction caused by RSV, can be used for reducing animal death cased by viral pneumonia, and can be used for remarkably protecting the immunity of experimental animals.

The invention provides a method for detecting a genotoxic agent by the utilization of Pseudorasbora parva and belongs to the field of chemicals safety evaluation and environmental monitoring. The method comprises the following steps: (1) processing Pseudorasbora parva by exposure treatment by the use of a water sample to be tested; and (2) carrying out single cell gel electrophoresis assay by using hepatic cells of Pseudorasbora parva processed after exposure treatment, detecting and counting cell tail length, DNA content and tail moment, and drawing a dose-effect curve. Through comparison between the result and a blank control and a positive control and through the dose-effect relationship, genetic toxicity of the test sample is determined. The detection method of the invention can sensitively and rapidly determine genetic toxicity of chemicals. The invention provides a China native species-based detection method for chemicals genetic toxicity evaluation and environmental genetic toxicity monitoring.

The invention provides a processing method and a processing device for efficacy of combined medicine. The processing method comprises: obtaining a dose-effect curved band of expected additive effect of combined medicine; obtaining an actual dose-effect relationship curve formed by variation of actual effect value of the combined medicine with variation of dosage of a certain target component medicine in the combined medicine; comparing position relationships of the actual dose-effect relationship curve and the dose-effect curved band, and when the actual dose-effect relationship curve is above the dose-effect curved band, efficacy output of the combined medicine being synergy; when the actual dose-effect relationship curve is below the dose-effect curved band, efficacy output of the combined medicine being antagonism; and when the actual dose-effect relationship curve is in the dose-effect curved band range, the efficacy output of the combined medicine being addition. The processing method solves a problem in the prior art that efficacy cannot be accurately detected when multiple medicines are used in a combined manner. The method can be widely applied in research and development of compound medicines, toxicologic study, efficacy and safety evaluation of the combined medicine, and environmental evaluation.

The invention discloses a single body effect target device suitable for measurement of target shock wave pressure of a movable explosion field. The effect target device is mainly characterized by being composed of a base, a response membrane, a pressing ring, a buffering pad, a bottom cover and pressing bolts; external shock waves act on the effect target device and a working area of the responsemembrane, so that the response membrane is plastically deformed and sunken towards a lower sealing hole, the maximal deflection value is measured, and the pressure peak of the shock waves is obtainedaccording to a dose-effect relationship of deformation of the membrane and the effect of the shock waves. The device has the significant advantages that the mass and size of the effect target device are greatly reduced, movement and disassembly are facilitated, the processing cost can be reduced at the same time, and the processing period can be shortened; the effect of fixing the response membrane is reinforced, the area where the response membrane is plastically deformed is controlled, and the measurement precision of the shock wave pressure is guaranteed on the condition that the mass of the device is reduced; a deflection testing unit interface is reserved for the response membrane, the upgradability of a shock wave pressure effect target of the movable explosion field is improved, andthe requirement of the target of the movable explosion field for the measurement of the shock wave pressure is met.

The invention discloses an application of Lambda interferon in resisting human immunodeficiency virus. IFN-Lambda resisting HIV infection can inhibit the infection and replication of HIV-1 in human macrophage, which is presented by the following processes: (1) IFN-Lambda1/Lambda2 differentiates mature human macrophage through pretreatment, then HIV-1R5 type strain respectively infects the cells, a DMEM culture medium is used for washing and then a DMEM culture medium containing 10 percent of fetal calf serum is added for culture; and (2) after infection, the formed multinucleate giant cells of IFN-Lambda treatment group are rather less than those of a virus contrast group, and the HIV-1 reverse transcriptase and envelope protein P24 in culture supernatant fluid of the IFN-Lambda treatment group are detected to be rather less than that of the virus contrast group. After infection, the HIV-1RT in the culture supernatant fluid is respectively detected. The effect of inhibiting the HIV-1 is obvious after infection. The IFN-Lambda treatment group has obvious time and dose-effect relationship in inhibiting the activity of HIV-1RT after infection. The function of IFN-Lambda in resisting the HIV-1 is similar to that of other interferons and has the antivirus effect. The IFN-Lambda shows obvious effect of inhibiting the replication of virus as regards to the human macrophage before, in and after the infection of the HIV-1 virus.

The invention discloses a method for detecting hydroxyl radicals in an earthworm body. Firstly, a fully exposed earthworm undergoes bowel clearing and is then cleaned. Radical PBN capture is carried out in a deoxygenated hermetical operation box. Finally, EPR technology is used for detection. The method has the advantages that the operation is relatively simple and easy to master and the hydroxylradicals of the earthworm can be detected within a short time; according to a hyperfine structure constant of an EPR spectrogram and the shape analysis of the spectrogram, the method discovers the detected radicals to be the hydroxyl radicals and is the most direct, accurate and effective method at present for detecting the hydroxyl radicals; and the method has a good dose-response relationship. Research results show that the strength of the hydroxyl radical and the exposed concentration are in good dose-response relationship.

The invention belongs to a biological indicator for rapid estimation of an ionizing radiation dose, and in particular relates to an application of soluble receptor tyrosine kinase AXL (sAXL) serving as the biological indicator for the rapid estimation of the ionizing radiation dose. After radiation, the concentration of the sAXL in serum or plasma is decreased along with the increase of the radiation dose, so that a good dose-effect relationship is formed after a period of radiation and can be detected rapidly by methods which are simple and easy to implement, such as ELISA (Enzyme Linked Immunosorbent Assay) and protein chips. During the detection, the required sample quantity is small; and the sampling is carried out conveniently. Thus, the sAXL can be taken as the biological indicator for the rapid estimation of the ionizing radiation dose. In addition, the ionizing radiation dose of a human body or an animal subjected to the ionizing radiation can be estimated by utilizing the content of the sAX in serum or plasma.