The invention relates to a preparation method of wheat straw nano-cellulose whiskers, belonging to the technical field of new materials. The invention treats wheat straw by steam explosion, breaks the wrapping effect of lignin and hemicellulose on cellulose, separates and removes most of hemicellulose and pectin, and uses sodium chlorite to oxidize and degrade lignin, and further removes it by alkali treatment Residue impurities, and then oxidize C6 on the primary hydroxyl group of cellulose to carboxyl group, combined with high-speed homogeneous dispersion method freeze-drying to produce small-sized high-purity needles grown in the form of single crystal structure, highly ordered atomic arrangement, internal Containing fewer defects, compared with ordinary natural cellulose, it has high specific surface area, high purity, high crystallinity, high strength, high modulus, ultrafine structure and high transparency of cellulose nano whiskers.