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Method of extracting and purifying melanin from almond peel

A technology of melanin and almonds, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problem of no melanin research reports

Inactive Publication Date: 2007-08-08
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] According to the data search done by the applicant, no relevant research report on the extraction of melanin has been found so far

Method used

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  • Method of extracting and purifying melanin from almond peel

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Embodiment 1

[0039] Embodiment 1: the process of extracting melanin with the seed coat of apricot as raw material

[0040] Weigh 5.0g of apricot seed coat, put it into a 150ml Erlenmeyer flask, add 75ml of 0.5mol / L NaOH aqueous solution, stir with a glass rod to fully immerse the apricot seed coat in the solution and remove the gas in the solution, and pass in nitrogen After 10 minutes the vial was sealed with a nitrogen-filled balloon. Put the triangular flask in a 60°C water bath for 12 hours and take it out. After the tap water is cooled, filter it with double-layer gauze. The filtrate is centrifuged at 15,000g for 15 minutes. ~2, store in 4°C refrigerator, add 75ml 0.5mol / L NaOH aqueous solution to filter the residue, continue to extract for 12 hours under nitrogen protection, then cool, roughly filter, filter the residue, wash and filter with 100ml deionized water, combine the filtrate and centrifuge , the supernatant was adjusted to a pH of 1-2 with 2mol / L hydrochloric acid, the two...

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Abstract

The invention discloses an extracting method and purifying method of melanin from almond peel, which comprises the following steps: adopting almond peel as raw material; extracting through alkaline solution; hydrolyzing impurity through acid; purifying through reciprocal organic solvent washing and alkaline dissolving to sediment; freezing; defreezing; washing through deionized water to remove Cl-; drying in the vacuum; obtaining the product.

Description

technical field [0001] The invention relates to the technical field of plant melanin production, in particular to a method for extracting and purifying melanin from plant materials. The melanin can be used in the fields of food and cosmetic industry, medicine, bioelectronics and the like. Background technique [0002] Melanin (Melanin) is a general term for a class of complex and diverse phenolic or indole biomacromolecular pigments that are ubiquitous in the biological world [Butler MJ, Day AW. Fungal melanins: A review. Canadian Journal of Microbiology, 1998, 44( 12):1115-1136.], which provide structural strength to organisms or protect organisms from environmental stresses such as ultraviolet rays, ionizing radiation, heavy metal pollution, low and high temperatures [Hovritt M, Steinbig Edited by Er A, translated by Guo Shengrong, Biopolymer Melanin, Volume I, Lignin, Humus and Coal. Beijing: Chemical Industry Press, 2004: 251-273.]. It is not only a class of photoprote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00
Inventor 赵忠姚增玉李科友马希汉
Owner NORTHWEST A & F UNIV
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