The invention relates to the technical field of biology, in particular to a migratory locust Rab5 gene and application of dsRNA of the migratory locust Rab5 gene in migratory locust prevention and treatment. Specifically, through a bioinformatics method, a fragment of the migratory locust small GTP enzyme gene 5 is obtained from a migratory locust transcriptome, a primer corresponding to the fragment is further designed and synthesized, PCR amplification is carried out, and after sequencing of an amplification product, the migratory locust Rab5 gene is obtained, and the sequence is shown as SEQ ID NO: 1. Furthermore, according to SEQ ID NO: 1, a corresponding primer is designed and synthesized, a gene segment with the nucleotide sequence shown in SEQ ID NO: 2 is obtained through PCR amplification, the gene segment is subjected to in-vitro transcription according to a kit specification to synthesize dsRNA, the dsRNA is injected into a migratory locust body cavity to specifically silence a target gene, migratory locust dies during ecdysis, and multiple experiments show that the fatality rate reaches 100%. Due to the specificity and the efficient fatality rate of the dsRNA, the dsRNA has important practical significance for migratory locust prevention and control, and a new way can be provided for migratory locust prevention and control.