Mulberry endophytic antagonistic bacteria new species-podosporia sp. and application thereof

A technology of sporophytes and endophytic fungi, applied in the fields of application, biocides, and microorganisms, can solve the problems of no high-yield, high-quality, disease-resistant fruit mulberry varieties, inability to prevent and control sclerotinia sclerotinia, and large labor input.

Active Publication Date: 2020-08-28
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the research on mulberry sclerotinia at home and abroad is on mulberry hypertrophic sclerotinia, but there are few reports on mulberry shrinking sclerotinia
At present, Sclerotinia sclerotiorum is mainly controlled by physical and chemical agents. However, physical control methods require a lot of labor, high cost, and limited control effect.
Chemical control will not only bring negative impacts such as environmental pollution and pesticide residues, but also make it difficult to guarantee the food safety of mulberries
And there is still no high-yield, high-quality, disease-resistant fruit mulberry variety that can be popularized and applied on a large scale in production.

Method used

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  • Mulberry endophytic antagonistic bacteria new species-podosporia sp. and application thereof
  • Mulberry endophytic antagonistic bacteria new species-podosporia sp. and application thereof
  • Mulberry endophytic antagonistic bacteria new species-podosporia sp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Isolation, purification and screening of endophytic antagonistic bacteria in mulberry trees

[0035] 1. Isolation and purification of mulberry endophytes

[0036] 1.1. Isolation of endophytes:

[0037] Collection and storage of samples: Collect mulberry stems from mulberry gardens, rinse them with tap water, dry them, store numbers at 4°C, and isolate endophytes in time.

[0038] Isolation of endophytes: cut the stems of mulberry trees into small sections (3.0-5.0 cm in length), soak them in 75.0% alcohol completely, take them out, ignite the alcohol on the surface of the stems on an alcohol lamp, and wait for the alcohol to burn out. Roll the mulberry branches sterilized on this surface on the blank medium, so that each surface of the mulberry branches is in contact with the medium, and use it as a blank control to verify whether the surface disinfection is thorough. Then it was placed in a sterile petri dish and cut open in three layers with a sterile sca...

Embodiment 2

[0044] Embodiment 2: the influence of temperature on the growth of antagonistic fungus 7GJ-4

[0045] Inoculate the pathogenic bacteria cake with a diameter of 5 mm on the center of the PDA plate, and culture it at 5°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, and 40°C, and measure the colony diameter with the cross method every day. Repeat 3 times for each group.

Embodiment 3

[0046] Embodiment 3: thermal stability, lethal effect of 7GJ-4 fermented liquid

[0047] 1. Detection of thermal stability of active fermentation liquid of antagonistic fungus 7GJ-4 strain

[0048] The aseptic fermentation filtrate of the antagonistic fungus 7GJ-4 was placed at 40°C, 60°C, 80°C, and 100°C for 30 minutes, and after cooling to room temperature, the hyphae growth rate method was used (the sterile fermentation filtrate was mixed at a volume of 1:4 Mix with sterilized PDA medium cooled to 40-50°C to prepare a detection plate, and mix with sterilized water and PDA medium at a volume ratio of 1:4 to prepare a control plate, and then separate the pathogenic bacteria cakes with a diameter of 5mm Inoculate in the center of the test plate and the control plate, culture at 22°C for several days, measure the colony diameter by the cross method, and repeat 3 times in each group. Calculate the antibacterial rate by comparing the colony diameter of the test plate with the col...

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PUM

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Abstract

The invention relates to mulberry endophytic fungi podosporia sp. 7GJ-4. The strain is collected in the China Center for Type Culture Collection on March 24, 2018 and has a collection number of CCTCCNO:M 2018118. The strain has the following advantages that endophytic fungi with the strong antagonistic effect on pathogenic bacteria of mulberry constrictive sclerotinios, namely mulberry scleromitrula shiraiana, are obtained by separation from a mulberry tree, and morphological and molecular biological identification proves that the strain is a new species of podosporia; the strain can grow within the temperature range of 15-30 DEG C, sterile fermentation filtrate has relatively high thermal stability, and the strain can adapt to the field temperature and has no lethal effect on mulberry tissue culture seedlings; and therefore, the strain has potential commercial development and application values, and has important application prospects in biological prevention and control practice ofmulberry sclerotinia.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a new strain of the mulberry tree antagonistic endophytic fungus Podospora. Background technique [0002] Mulberry sclerotinia, commonly known as ginkgo disease, is a fruit mulberry disease caused by fungi. The diseased fruit is off-white, has a foul smell, and loses its commodity and edible value. It occurs in fruit and mulberry planting areas such as Southwest China, Jiangsu and Zhejiang, and the southeast coast, and the incidence rate can be as high as 30% to 90%. In some areas, thousands of acres of mulberry orchards have lost mulberry production due to the disease. According to different symptoms, the disease is divided into mulberry hypertrophic sclerotinia, mulberry small-grained sclerotinia and mulberry shrinking sclerotinia, which are composed of Ciboriashiraiana and Ciboriacarunculoides respectively. and Mori core ground cod (Sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCA01N63/30C12R2001/645C12N1/145
Inventor 谢洁任慧爽
Owner SOUTHWEST UNIVERSITY
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