Mulberry endophytic antagonistic bacteria new species-podosporia sp. and application thereof

Abstract

The invention relates to mulberry endophytic fungi podosporia sp. 7GJ-4. The strain is collected in the China Center for Type Culture Collection on March 24, 2018 and has a collection number of CCTCCNO:M 2018118. The strain has the following advantages that endophytic fungi with the strong antagonistic effect on pathogenic bacteria of mulberry constrictive sclerotinios, namely mulberry scleromitrula shiraiana, are obtained by separation from a mulberry tree, and morphological and molecular biological identification proves that the strain is a new species of podosporia; the strain can grow within the temperature range of 15-30 DEG C, sterile fermentation filtrate has relatively high thermal stability, and the strain can adapt to the field temperature and has no lethal effect on mulberry tissue culture seedlings; and therefore, the strain has potential commercial development and application values, and has important application prospects in biological prevention and control practice ofmulberry sclerotinia.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a new strain of the mulberry tree antagonistic endophytic fungus Podospora. Background technique [0002] Mulberry sclerotinia, commonly known as ginkgo disease, is a fruit mulberry disease caused by fungi. The diseased fruit is off-white, has a foul smell, and loses its commodity and edible value. It occurs in fruit and mulberry planting areas such as Southwest China, Jiangsu and Zhejiang, and the southeast coast, and the incidence rate can be as high as 30% to 90%. In some areas, thousands of acres of mulberry orchards have lost mulberry production due to the disease. According to different symptoms, the disease is divided into mulberry hypertrophic sclerotinia, mulberry small-grained sclerotinia and mulberry shrinking sclerotinia, which are composed of Ciboriashiraiana and Ciboriacarunculoides respectively. and Mori core ground cod (Sc...

AI Technical Summary

Problems solved by technology

However, most of the research on mulberry sclerotinia at home and abroad is on mulberry hypertrophic sclerotinia, but there are few reports on mulberry shrinking sclerotinia

At present, Sclerotinia sclerotiorum is mainly controlled by physical and chemical agents. However, physical control methods require a lot of labor, high cost, and limited control effect.

Chemical control will not only bring negative impacts such as environmental pollution and pesticide residues, but also make it difficult to guarantee the food safety of mulberries

And there is still no high-yield, high-quality, disease-resistant fruit mulberry variety that can be popularized and applied on a large scale in production.

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