Sansevieria trifasciata polysaccharides as well as preparation method and application thereof

A technology of tiger piranha and blue polysaccharides, applied in the field of tiger piranha polysaccharides and its preparation, can solve the problems that there is no major breakthrough in the structure and structure-activity relationship of polysaccharides

Inactive Publication Date: 2012-10-24
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some progress has been made in domestic research on the separation and purification of polysaccharides, biological activity and pharmacology, there is still no major breakthrough in the research on the structure and structure-activity relationship of polysaccharides.

Method used

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  • Sansevieria trifasciata polysaccharides as well as preparation method and application thereof
  • Sansevieria trifasciata polysaccharides as well as preparation method and application thereof
  • Sansevieria trifasciata polysaccharides as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Take tiger Pilan dry powder, extract with petroleum ether, reflux at 90°C until the extract is almost colorless, and then reflux with 80% ethanol at 60°C for 2h.

[0038] 2) Use DK-98-1 constant temperature water bath (Tianjin Taisite Instrument Co., Ltd.) to extract, under the conditions of extraction temperature of 90°C, extraction time of 160min, and solid-liquid ratio of 1:30, hot water extraction is used.

[0039] 3) The extract was concentrated under reduced pressure by a RE-52C rotary evaporator (Shanghai Qingpu Instrument Factory), and 3 times the volume of 95% ethanol was added to precipitate overnight at 4°C.

[0040] 4) The precipitate was centrifuged in a DL-5-B centrifuge (Shanghai Anting Scientific Instrument Factory), washed with absolute ethanol, acetone, and anhydrous ether in sequence, and finally passed through a FD-1B-50 vacuum freeze dryer (Beijing Boyikang) Experimental Instrument Co., Ltd.) freeze-dried to obtain the crude polysaccharide of Tiger...

Embodiment 2

[0041] Example 2 The crude polysaccharide obtained by hot water extraction is deproteinized by enzymatic method combined with Sevage method

[0042] 1) Adjust the pH of the polysaccharide solution to be deproteinized to 8.0, add trypsin in an amount of 2%, act at 37°C for 2 hours, stir at intervals, and then rise to 80°C to inactivate the enzyme.

[0043] 2) Centrifuge the treated extract at 4000r / min for 10min, take the supernatant and concentrate, deproteinize by Sevag method, repeat 3 times.

[0044] 3) Use the dialysis method to dialyze the deproteinized tiger Piran polysaccharide concentrate with distilled water for 48 hours at 4°C, change it several times in the middle, concentrate after dialysis, precipitate with 3 times 95% ethanol, centrifuge and vacuum freeze-dry to obtain preliminary Purification of Tiger Piran polysaccharide TPS.

Embodiment 3

[0045] Example 3 The deproteinized polysaccharide is purified by DEAE-52 column chromatography:

[0046] 1) Select a chromatographic column with a specification of 3.2cm×40cm, use 3-5 times the column volume of deionized water to run the column under constant pressure, and pass through the chromatographic column at a flow rate of 1ml / min.

[0047] 2) Take the deproteinized crude polysaccharide and dissolve it in a small amount of distilled water, use 0.5g sample volume, and the sample volume is 10ml.

[0048] 3) Elute with deionized water and 0.05, 0.1, 0.2, 0.3mol / L NaCl solution in sequence, control the flow rate at 0.75ml / min, and collect the eluate with SBS-100 automatic partial collector (Shanghai Huxi Instrument Factory), Collect approximately 10ml per tube.

[0049] 4) Combine and collect the elution peaks of I, II, III, IV, and V respectively, concentrate, and dialyze to AgNO 3 Vacuum freeze-dry until no white precipitate is detected. The five elution peaks are re...

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Abstract

The invention discloses Sansevieria trifasciata polysaccharides as well as a preparation method and application of the Sansevieria trifasciata polysaccharides. The Sansevieria trifasciata polysaccharides are prepared by the steps of: drying Sansevieria trifasciata leaf, crushing, screening, adding a certain volume of petroleum ether, refluxing with ethanol to remove impurities, carrying out suction filtering on the extract to obtain filter residue, and airing the filter residue for standby; extracting polysaccharides of Sansevieria trifasciata by a hot water leaching method, deproteinizing crude polysaccharides by combining an enzyme method with a Sevag method, and separating and purifying the deproteinized Sansevieria trifasciata polysaccharides TPS is by DEAE-52 column chromatography and SephdexG-100 column chromatography to respectively obtain four uniform components: TPS-A, TPS-B, TPS-C and TPS-H. TPS, TPS-H and TPS-A have obvious inhibitory action on the growth of human lung cancer cell A549, human nasopharyngeal carcinoma cell line CNE and human skin T cell lymphomata Hut102, and TPS-A has the best effect; and TPS, TPS-H and TPS-A have no inhibitory action on human colon cancer cell LoVo cell, and have no lethal effect on human intact liver cell LO2, thereby providing scientific reference for research and development of targeted antitumor medicaments.

Description

technical field [0001] The present invention relates to the extraction of plant active ingredients, in particular the tiger piperan polysaccharide and its preparation method and application. Background technique [0002] Tiger Piran ( Sansevieria trifasciata ), also known as Phnom Penh Tiger Pilan, a typical Sansevieria plant of the Agaveaceae family. This plant originates from West Africa and is an evergreen perennial herb that is distributed in the tropics and subtropics and has strong adaptability to the environment. [0003] Tiger Pilan has rhizomes, firm and upright leaves, firm posture, sword-shaped leaves, dark green leaves with yellow band-like stripes on the edges. It has many varieties, and its plant shape and leaf color change greatly. It is exquisite and unique. It is an indoor ornamental plant suitable for potted plants. It can survive even in low light and low water conditions. In addition, because tiger Pilan leaves contain a lot of hemp fibers, the leaves ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
Inventor 李泽鸿张帆张继元刘洪章张璐刘淑英管玉兵陶英楠李昌昊刘文涛于新海史飞
Owner JILIN AGRICULTURAL UNIV
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