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Method for detecting genotoxic agent in water by utilization of Pseudorasbora parva

A technology of genotoxicants and wheat ear fish, which is applied in the direction of testing water, measuring devices, material inspection items, etc.

Inactive Publication Date: 2016-04-20
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no method for the evaluation of genotoxicity using the comet assay of the earfish

Method used

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  • Method for detecting genotoxic agent in water by utilization of Pseudorasbora parva
  • Method for detecting genotoxic agent in water by utilization of Pseudorasbora parva
  • Method for detecting genotoxic agent in water by utilization of Pseudorasbora parva

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Embodiment

[0029] Water samples from a section of the Hunhe River were collected, filtered through glass fiber membranes, and then used for the exposure of wheat ear fish; potassium dichromate (K 2 Cr 2 o 7 ) and 4-nitroquinoline-1-oxide (4-NQO) were used as positive controls to evaluate the genotoxicity of the water sample to the earfish.

[0030] This specific embodiment adopts the following steps:

[0031] (1) The oysters were domesticated for one week in the laboratory, during which the mortality rate was less than 5%, and the feeding was stopped the day before the exposure, and the same batch of oysters with similar body length and weight were selected for the experiment;

[0032] (2) Use tap water that has been aerated for more than one day to prepare the exposed water samples of wheat ear fish: positive control chemical, K 2 Cr 2 o 7 The concentration gradient is 2.5mg / L, 5mg / L, 10mg / L, and the 4-NQO concentration gradient is 0.2μg / L, 0.5μg / L, 1μg / L; the environmental water s...

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Abstract

The invention provides a method for detecting a genotoxic agent by the utilization of Pseudorasbora parva and belongs to the field of chemicals safety evaluation and environmental monitoring. The method comprises the following steps: (1) processing Pseudorasbora parva by exposure treatment by the use of a water sample to be tested; and (2) carrying out single cell gel electrophoresis assay by using hepatic cells of Pseudorasbora parva processed after exposure treatment, detecting and counting cell tail length, DNA content and tail moment, and drawing a dose-effect curve. Through comparison between the result and a blank control and a positive control and through the dose-effect relationship, genetic toxicity of the test sample is determined. The detection method of the invention can sensitively and rapidly determine genetic toxicity of chemicals. The invention provides a China native species-based detection method for chemicals genetic toxicity evaluation and environmental genetic toxicity monitoring.

Description

technical field [0001] The invention provides a method for detecting genotoxic substances in water by using the ear fish, which belongs to the fields of chemical safety evaluation and environmental monitoring. Background technique [0002] The genotoxicity test and evaluation methods have been included in the prescribed test items involving human health or environmental risk assessment, such as new drug research, environmental monitoring, pesticide development, chemical substance and food safety evaluation, and new material development. Due to the regional nature of biological species, the use of international standard model species for environmental monitoring and chemical safety assessment cannot fully protect my country's native organisms. Therefore, my country's new chemical substance registration regulations stipulate that the ecotoxicology test data completed in China with Chinese test organisms must be provided when new chemical substance notification is made. It is ...

Claims

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Application Information

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IPC IPC(8): G01N33/18G01N21/84
CPCG01N33/186G01N21/84
Inventor 刘薇张洲张新赵璐璐杨京亚
Owner DALIAN UNIV OF TECH
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